H-2 Antigens pp 201-208 | Cite as

Regulatory Elements in the Promoter of the H-2Dd Class I Gene

  • Bette Korber
  • Leroy Hood
  • Iwona Stroynowski
Part of the NATO ASI Series book series (NSSA, volume 144)


Murine class I antigen expression undergoes modulation during development and adult life. Early embryonic cells do not transcribe detectable amounts of class I and β2 microglobulin mRNA and express little, if any, H-2 antigens (1,2). In contrast, adult cells express class I mRNA constitutively and ubiquitously but the relative basal level of class I antigen expression varies on different cells and tissues (reviewed in 3). The quantity of various class I antigens expressed undergoes additional changes upon exposure to agents such as type I and type Ilinterferons and tumor necrosis factor (4–6). Since quantitative variation in class I protein expression can influence the acquisition of tolerance, MHC restriction, and the efficiency of cell-mediated immune responses we were interested in identifying and characterizing the DNA elements and trans-acting factors which are important for the regulation of class I gene expression. This information may be eventually applied for therapeutic purposes to modulate class I protein levels and to affect the immune response in vivo.


Nuclear Extract TATA Motif Cell Nuclear Extract Thymidine Kinase Promoter CCAAT Motif 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    D. Morello, P. Duprey, A. Israel, and C. Babinet, Asynchronous regulation of mouse I-1–2D and beta-2-microglobulin RNA transcripts, Immunogenetics 22: 441–452 (1985).Google Scholar
  2. K. Ozato, Y.-J. Wan, and B. M. Orrison, Mouse major histocompatibility class I gene expression begins at midsomite stage and is inducible in earlier-stage embryos by interferon, Proc. Natl. Acad. Sci. USA 82:2427–2431 (1985).Google Scholar
  3. 3.
    H. W. Harris, and T. J. Gill III, Expression of class I transplantation antigens, Transplantation 42: 109–116 (1986).Google Scholar
  4. 4.
    F. Rosa, and M. Fellous, The effect of gamma-interferon on MHC antigens, Immunology Today 5: 261–262 (1984).Google Scholar
  5. 5.
    O. Yoshie, H. Schmidt, E. Shyam, P. Reddy, S. Weissman, and P. Lengyel, Mouse interferons enhance the accumulation of human HLA RNA and protein in transfected mouse and hamster cells. J. Biol. Chem/ 257: 13169–13172 (1982).Google Scholar
  6. 6.
    T. Collins, L. A. Lapierre, W. Lapierre, J. L. Strominger, and J. S. Pober, Recombinant human necrosis factor increases mRNA levels and surface expression of HLA-A,B antigens in vascular endothelial cells and dermal fibroblasts in vitro, Proc. Natl. Acad. Sci. USA 83:446–450 (1986).Google Scholar
  7. 7.
    A. Kimura, A. Israel, O. Le Bail, and P. Kourilsky, Detailed analysis of the mouse H-2Kb promoter: enhancer-like sequences and their role in the regulation of class I gene expression, Cell 44: 261–272 (1986).PubMedCrossRefGoogle Scholar
  8. 8.
    J. Miyazaki, E. Appella, and K. Ozato, Negative regulation of the major histocompatibility class I gene in undifferentiated embryonal carcinoma cells, Proc. Natl. Acad. Sci. USA 83:9537–9541 (1986).PubMedCrossRefGoogle Scholar
  9. 9.
    A. Israel, A. Kimura, A. Fournier, M. Fellous, and P. Kourilsky, Interferon response sequence potentiates activity of an enhancer in the promoter region of a mouse H-2 gene, Nature (London) 322: 743–746 (1986).Google Scholar
  10. 10.
    B. Korber, L. Hood, and I. Stroynowski, Regulation of murine class I genes by interferons is controlled by regions located both 5’ and 3’ to the transcription initiation site, Proc. Natl. Acad. Sci. USA 84: 3380–3384 (1987).Google Scholar
  11. 11.
    J. Vogel, M. Kress, G. Khoury, and G. Jay, A transcriptional enhancer and and interferon-responsive sequence in major histocompatibility complex class I genes, Mol. Cell. Biol. 6: 3550–3554 (1986).PubMedCentralPubMedGoogle Scholar
  12. 12.
    T. Maniatis, S. Goodbourn, and J. A. Fischer, Regulation of inducible and tissue-specific gene expression, Science 236: 1237–1244 (1987).Google Scholar
  13. 13.
    A. S. Baldwin, Jr. and P. A. Sharp, Binding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2K class I major histocompatibility gene, Mol. Cell. Biol. 7: 305–313 (1987).PubMedCentralPubMedGoogle Scholar
  14. 14.
    A. Israel, A. Kimura, M. Kieran, O. Yano, J. Kanellopoulos, O. Le Bail, and P. Kourilsky, A common positive trans-acting factor binds to enhancer sequences in the promoters of mouse H-2 and beta-2-microglobulin genes, Proc. Natl. Acad. Sci. USA 84: 2653–2657 (1987).PubMedCrossRefGoogle Scholar
  15. 15.
    B. Korber, L. Hood, and I. Stroynowski, Regulation of gene expression by interferons: cell-type and multi-site control of H-2 promoter responses (submitted) (1987).Google Scholar
  16. 16.
    W. Lee, P. Mitchell, and R. Tjian, Purified transcription factor AP-1 binds to TPA-inducible enhancer elements, Cell 49: 741–752 (1987).PubMedCrossRefGoogle Scholar
  17. 17.
    R. Breathnach, and P. Chambon, Organization and expression of eukaryotic split genes, Ann. Rev. Biochem. 50: 349–383 (1981).CrossRefGoogle Scholar
  18. 18.
    P. Mellon, V. Parker, Y. Gluzman, and T. Maniatis, Identification of DNA sequences required for transcription of the human al globin gene in a new SV40 host-vector system, Cell 27: 279–288 (1981).PubMedCrossRefGoogle Scholar
  19. 19.
    K. A. Jones, J. T. Kadonaga, P. J. Rosenfeld, T. J. Kelly, and R. Tjian, A cellular DNA binding protein that activates eukaryotic transcription and DNA replication, Cell 48: 79–89 (1987).PubMedCrossRefGoogle Scholar
  20. 20.
    M. Sawadogo, and R. G. Roeder, Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay, Proc. Natl. Acad. Sci. USA 82: 4394–4398 (1985).PubMedCrossRefGoogle Scholar
  21. 21.
    C. S. Parker, and J. Topol, A Drosophila RNA polymerae II transcription factor contains a promoter-region-specific DNA-binding activity, Cell 36: 357–369 (1984).PubMedCrossRefGoogle Scholar
  22. 22.
    D. J. Galas, and A. Schmitz, DNase footprinting: a simple method for the detection of protein-DNA binding specificity. Nucl. Acids Res. 5: 3157–3170 (1978).PubMedCrossRefGoogle Scholar
  23. 23.
    M. Sawadogo, and R. G. Roeder, Interaction of a gene specific transcription factor with the adenovirus major late promoter upstream of the TATA box region, Cell 43: 165–175 (1985).Google Scholar
  24. 24.
    J. Lallane, C. Delarbre, G. Grachelin, and P. Kourilsky, A cDNA clone containing the entire coding sequence of a mouse H-2K histocompatibility antigen, Nucleic Acids Res. 1: 1567–1577 (1983).Google Scholar
  25. 25.
    B. Arnold, H. G. Burgert, A. L. Archibald, and S. Kvist, Complete nucleotide seugence of the murine H-2K gene. Comparison of three H-2K locus alleles. Nucl. Acids Res. 12: 9473–9487 (1984).PubMedCrossRefGoogle Scholar
  26. 26.
    S. Kvist, L. Roberts, and B. goberstein, Mouse histocompatibility genes: structure and organization of a K gene, EMBO J. 2: 245–254 (1983).Google Scholar
  27. 27.
    B. J. Graves, P. F. Johnson, and S. L. McKnight, Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk gene, Cell 44: 565–576 (1986).PubMedCrossRefGoogle Scholar
  28. 28.
    K. A. Jones, K. R. Yamamoto and R. Tjian, Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro, Cell 42: 559–572, (1985).PubMedCrossRefGoogle Scholar
  29. 29.
    S. McKnight, R. Tjian, Transcriptional Selectivity of Viral Genes in Mammalian Cells, Cell 46: 759–805.Google Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Bette Korber
    • 1
  • Leroy Hood
    • 1
  • Iwona Stroynowski
    • 1
  1. 1.Division of BiologyCalifornia Institute of TechnologyPasadenaUSA

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