H-2 Antigens pp 157-168 | Cite as

Toward an Understanding of the Biochemical Nature of H-2 Molecules: A Historical Perspective

  • Stanley G. Nathenson
Part of the NATO ASI Series book series (NSSA, volume 144)


As part of an historical perspective of the development of our knowledge of the H-2 genes and products, this paper presents a brief, selected, personal account of those studies from my laboratory which were carried out with the objective to understand the biochemical nature of H-2 molecules. This work was performed with the goal of relating the structural features of what are now called class I MHC molecules to their biological function, in addition to expanding our understanding of the genetic organization and diversity of the H-2 region genes.


Cyanogen Bromide Antigenic Activity Biochemical Nature Transplantation Antigen Monospecific Antiserum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Nathenson, S.G. Biochemical properties of histocompatibility antigens, Ann. Rev. Genetics 4:69 (1970).Google Scholar
  2. 2.
    Sanderson, A.R., Cytotoxic reactions of mouse isoantisera; scope of an assay using radiolabeled target cells, Transplantation 3: 557 (1965).Google Scholar
  3. 3.
    Wigzell, R. Quantitative titration of mouse H-2 antibodies using Cr labeled target cells, Transplantation 3: 423 (1965).Google Scholar
  4. 4.
    Shimda, A. and Nathenson, S.G., Murine histocompatibility-2 (H-2) alloantigens: Purification anal some chemical properties of soluble products from H-2 and H-2 genotypes released by papain digestion of membrane fractions, Biochemistry 8: 4048 (1969).Google Scholar
  5. 5.
    Muramatsu, T. and Nathenson, S. G., Studies on the carbohydrate portion of membrane-located mouse H-2 alloantigens, Biochemistry 9: 4875 (1970).Google Scholar
  6. 6.
    Rask, L., Lindblom J.B. and Peterson, P.A., Subunit structure of H-2 alloantigens, Nature 249: 833 (1974).Google Scholar
  7. 7.
    Silver, J. and Hood, L., Detergent-solubilized H-2 alloantigen is associated with a small molecular weight polypeptide, Nature 249: 764 (1974).Google Scholar
  8. 8.
    Schwartz, B.D. and Nathenson, S.G., Isolation of H-2 alloantigens solubilized by the detergent NP-40, J. Immunol. 107:1363 (1971.Google Scholar
  9. 9.
    Snell, G.D., Cherry, M. and Demant, P., Evidence that H-2 private specificities can be arranged in two mutually exclusive systems possibly homologous with two subsystems of HL-A, Transpl. Proc. 3:183 (1971).Google Scholar
  10. 10.
    Cullen, S., Schwartz, B.D., Nathenson, S.G. and Cherry, M., The molecular basis of codominant expression of the histocompatibility-2 genetic region, Proc. Natl. Acad. Sci. USA 69:1394 (1972).Google Scholar
  11. 11.
    Cullen, S.E., David, C.S., Shreffler, D.C. and Nathenson, S.G., Membrane molecules determined by the H-2 associated immune response region: Isolation and some properties, Proc. Natl. Acad. Sci. USA 71:648 (1974).Google Scholar
  12. 12.
    Brown, L., Kato, K., Silver, J. and Nathenson, S.G. Notable diversity in peptide composition of murine H-2K and H-2D alloantigens, Biochemistry 13: 3174 (1974).Google Scholar
  13. 13.
    Ewenstein, B.M., Freed, J.H., Mole, L.E. and Nathenson, S.G., Localization of the papain cleavage site of H-2 glycoproteins, Proc. Natl. Acad. Sci. USA 73:915 (1976).Google Scholar
  14. 14.
    Nathenson, S.G., Uehara, H., Ewenstein, B.M., Kindt, T.J. and Coligan, J.E., Primary structural analysis of the transplantation antigens of the murine H-2 major histocompatibility complex, Ann. Rev. Biochem. 50:1025 (1981).Google Scholar
  15. 15.
    Strominger J.L. Engelhard, V.H., Fuks, A., Guild, B.C., Hyafil, F., Kauffman, J.F., Korman, A.J., Kostyk, T.G., Krangel, M.S.., Lancet, D., Lopez de Castro, J.A., Mann, D.L., Orr, H.T., Parham, P., Parker, K.C., Ploegh, H.L., Pober, J.S., Robb, R.J., and Shackelford, D.A., Biochemical analysis of products of the MHC. in “The Role of the Major Histocompatibility Complex in Immunobiology,” M.E. Dorf, ed., Garland, New York (1981).Google Scholar
  16. 16.
    Steinmetz, M., Frelinger, J.G., Fisher, D., Hunkapiller, T., Periera, D., Weissman, S.M., Uehara, H., Nathenson, S.G. and Hood, L., Three cDNA clones encoding mouse transplantation antigens: Homology to immunoglobulin genes, Cell 24: 134 (1981).Google Scholar
  17. 17.
    Yokoyama, K. and Nathenson, S.G., Intramolecular organization of class I H-2 antigens: Localization of the alloantigenic determinants and the ß2 binding site to different regions of the H-2 K glycoprotein, J. Immunol. 130:1419 (1983).Google Scholar
  18. 18.
    Egorov, I., Genetic control of H-2 alloantigens as inferred from analysis of mutations, Immunogenetics 1: 97 (1974).Google Scholar
  19. 19.
    Bailey, D.W. and Kohn, H., Inherited histocompatibility changes in progeny of irradiated and unirradiated inbred mice, Genet. Res. 6: 330 (1965).Google Scholar
  20. 20.
    Nairn, R., Yamaga, K. and Nathenson, S.G., Biochemistry of the gene products from murine MHC mutants, Ann. Rev. Genetics 14:241 (1980).Google Scholar
  21. 21.
    Nathenson, S.G., Brown, J.L., Ewenstein, B.M., Nisizawa, T., Sears, D.W., and Freed, J.H. Structural differences between parent and variant H-2K glycoproteins from mouse strains carrying H-2 gene mutations, in Cold Spring Harbor Symposia on Quantitative Biology, Cold Spring Harbor Laboratory, New York, Vol. XLI, 1977, p. 343.Google Scholar
  22. 22.
    Pease, L.R., Schulze, D.H., Pfaffenbach, G.M. and Nathenson, S.G., Spontaneous H-2 mutants provide evidence that a copy mechanism analogous to gene conversion generates polymorphism in the major histocompatibility complex, Proc. Natl. Acad. Sci. 80:242 (1983).Google Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Stanley G. Nathenson
    • 1
  1. 1.Department of Microbiology and Immunology and Department of Cell BiologyAlbert Einstein College of MedicineBronxUSA

Personalised recommendations