Involvement of the H-2 Complex in Steroid Hormone Metabolism
We investigated DNA from mice with different H-2 haplotypes (b. d., f, j, k, p, g, r, s, u, v,). DNA was digested with five restriction enzymes (Eco RI, Taq I, Pvu II, Hind III, and Bgl II) and probed with human 21-OHase cDNA clone. Combined restriction fragment length polymorphism identify six different patterns of endonuclease fragment polymorphism of the murine 21-OHase region. Furthermore, we have investigated the serum level of two steroid hormones (progesterone and corticosterone) in the metabolic pathway involving the 21-OHase. Serum progesterone and corticosterone concentrations exhibit variations according to the H-2 haplotypes of the mice, suggesting that genetic factor(s) identical to or closely linked to the H-2 complex is involved in the control of their metabolism.
KeywordsMajor Histocompatibility Complex Folia Biol Serum Progesterone Concentration Major Histocompatibility Complex Restriction Serum Corticosterone Concentration
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