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Do Hyperplastoid Cell Lines “Differentiate Themselves to Death”?

  • Chapter
Cell Impairment in Aging and Development

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 53))

Abstract

Hayflick and Moorhead (1) clearly differentiated between two classes of mammalian cell lines: 1) Those typified by HeLa are apparently immortal and may serve as models for the study of neoplastic cell proliferation; we refer to them as “neoplastoid” 2) Those typified by WI-38 and by human skin fibroblast cultures eventually cease replicating and may be useful as models for the study of hyperplastic cellular proliferation or wound healing; consequently, we refer to them as “hyperplastoid.” Martin and Sprague (2) have recently tabulated some 21 parameters which have been claimed to differentiate between these two classes of cell lines. In mass cultures, the replicative life-span is currently among the most unambiguous differential parameters. Individual clones of either type of culture may cease proliferating, however, and it is this phenomenon which we refer to as “clonal senescence.” In the case of human diploid somatic cells, it is probable that some thousands of such clones have been followed in many different laboratories and to the best of our knowledge, all of them eventually stop growing, unless they are induced to undergo malignant transformation. Curiously, much less is known about the replicative life histories of individual clones and sub-clones of neoplastoid cells, even though they are comparatively easy to clone.

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Author information

Authors and Affiliations

  1. Depts. of Pathology, Biochemistry and Medicine, University of Washington, Seattle, Washington, USA

    G. M. Martin, C. A. Sprague, T. H. Norwood, W. R. Pendergrass, P. Bornstein, H. Hoehn & W. P. Arend

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  1. G. M. Martin
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  2. C. A. Sprague
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  3. T. H. Norwood
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  4. W. R. Pendergrass
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  5. P. Bornstein
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  6. H. Hoehn
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  7. W. P. Arend
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Editors and Affiliations

  1. Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania, USA

    Vincent J. Cristofalo

  2. Institute of Physiology, Czechoslovak Academy of Sciences, Budějovická 1083, 142 20, Prague 4, Czechoslovakia

    Emma Holečková

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Martin, G.M. et al. (1975). Do Hyperplastoid Cell Lines “Differentiate Themselves to Death”?. In: Cristofalo, V.J., Holečková, E. (eds) Cell Impairment in Aging and Development. Advances in Experimental Medicine and Biology, vol 53. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0731-1_6

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  • DOI: https://doi.org/10.1007/978-1-4757-0731-1_6

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  • Online ISBN: 978-1-4757-0731-1

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