Do Hyperplastoid Cell Lines “Differentiate Themselves to Death”?

  • G. M. Martin
  • C. A. Sprague
  • T. H. Norwood
  • W. R. Pendergrass
  • P. Bornstein
  • H. Hoehn
  • W. P. Arend
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 53)


Hayflick and Moorhead (1) clearly differentiated between two classes of mammalian cell lines: 1) Those typified by HeLa are apparently immortal and may serve as models for the study of neoplastic cell proliferation; we refer to them as “neoplastoid” 2) Those typified by WI-38 and by human skin fibroblast cultures eventually cease replicating and may be useful as models for the study of hyperplastic cellular proliferation or wound healing; consequently, we refer to them as “hyperplastoid.” Martin and Sprague (2) have recently tabulated some 21 parameters which have been claimed to differentiate between these two classes of cell lines. In mass cultures, the replicative life-span is currently among the most unambiguous differential parameters. Individual clones of either type of culture may cease proliferating, however, and it is this phenomenon which we refer to as “clonal senescence.” In the case of human diploid somatic cells, it is probable that some thousands of such clones have been followed in many different laboratories and to the best of our knowledge, all of them eventually stop growing, unless they are induced to undergo malignant transformation. Curiously, much less is known about the replicative life histories of individual clones and sub-clones of neoplastoid cells, even though they are comparatively easy to clone.


Skin Fibroblast Terminal Differentiation Abnormal Protein Human Diploid Cell Chang Liver Cell 
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Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • G. M. Martin
    • 1
  • C. A. Sprague
    • 1
  • T. H. Norwood
    • 1
  • W. R. Pendergrass
    • 1
  • P. Bornstein
    • 1
  • H. Hoehn
    • 1
  • W. P. Arend
    • 1
  1. 1.Depts. of Pathology, Biochemistry and MedicineUniversity of WashingtonSeattleUSA

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