Ultrastructure of in Vitro Aged Chick Embryo Cell Cultures in Relation to Viral Infection

  • Fedor Cǐampor
  • Helena Líbíková
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 53)


The ultrastructure of young uninfected and infected with herpes simplex virus chick embryo cells was compared with that aged cells. In contrast to the nuclei of young chick embryo cells 24 hr after infection, the aged chick embryo cells 24 hr after infection presented very expressive blocks of perinuclear chromatin. The nucleoplasm contains forming naked HSV particles. The lesions seen are dilation of the perinuclear cisternae, dilatation of rough endoplasmic reticulum cisternae and formation of multilamellar bodies. The moderate swellings of the mitochondria with violation of cristae arrangement and losing of mitochondrial matrix were observed. In contrast to the cytoplasm of young infected cells no formation of crystal-like arrangement of cytoplasmic ribosome tetrads occurs.


Herpes Simplex Virus Rough Endoplasmic Reticulum Young Culture Abortive Infection Chick Embryo Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Brock, M.A. and Hay, R.J. 1971. Comparative ultrestructure of chick fibroblasts in vitro at early and late stages during their growth span. J. Ultrastruct. Res. 36: 291Google Scholar
  2. 2.
    Ciampor, F. An electron microscopic study of influenza virus reproductive cycle in chick embryo cells with selective inhibition of host cell macromolecular synthesis. Acta Viral., in press.`Google Scholar
  3. 3.
    Krug,R.M, 1971. Influenza viral RNPs newly synthesized during the latent period of viral growth in MDCK cells. Virology 44: 125.Google Scholar
  4. 4.
    Libikovâ, H. 1973. Interferon in, young and aged chick embryo cell cultures infected with herpes simplex and pseudorabies viruses. Acta Virol. 17: 464.PubMedGoogle Scholar
  5. 5.
    Nií, S., Morgan, C. and Rose, H.M. 1968. Electron microscopy of herpes simplex virus. II. Sequence of development. J. Viral. 2: 517.Google Scholar
  6. 6.
    Nil, S. 1971. infected Electron microscopic observations on FL cells herpes simplex infected with virus. I. Viral forms. Biken J. 14: 177.Google Scholar
  7. 7.
    Níi, S. 1971. Electron microscopic observations on FL cells infected with Electron microscopic observations on FL cells J. 14: 325.Google Scholar
  8. 8.
    Shipkey, F.H., Erlandson, R.A., Bailey, R.B., Babcock, V.I. and Southan, C.M. 1967. Virus biographies.. I I. Growth of herpes simplex virus in tissue culture. Exptl. Molec.Google Scholar
  9. 9.
    Spring, S.B. and Roizman, B. 1967. Herpes simplex virus products in productive and abortive infection- Stabili- zation with formaldehyde and preliminary analyses by isopycnic centrifugation in CsCl. J. Viral 1: 294.Google Scholar
  10. 10.
    Spring, S.B.,’Roizman, B. and Schwartz, J. 1968. Herpes simplex virus products in productive and abortive infection.’ II. Electron microscopic and immunological evidence for failure of virus envelopment as a cause of abortive infection. J. Viral. 2: 384.’Google Scholar
  11. Strandberg, J,D, and Aurelian, L. 1969. Replication of canine herpes virus. II. Virus development and release in infected, dog kidney cells. J. V roi. 4: 480, 1969.Google Scholar
  12. Venable, J.H. and Coggeshall, R. 1965. A simplified lead citrate stain for use in electron microscopy,, J. Cell Bio1. 22: 407.Google Scholar

Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Fedor Cǐampor
    • 1
  • Helena Líbíková
    • 1
  1. 1.Institute of VirologySlovak Academy of ScienceBratislavaCzechoslovakia

Personalised recommendations