The Use of Arrested Populations of Human Diploid Fibroblasts for the Study of Senescence in Vitro

  • Robert T. Dell’Orco
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 53)


In recent years attempts have been made to equate in vivo senescence with the finite lifespan demonstrated by human diploid cells. in vitro (1). While preliminary evidence indicates that it may be possible to use cultured cells as an in vitro model system for cellular aging, several critical questions need to be resolved before any true parallel can be drawn between the in vitro phenomenon and the in vivo process.


Cell Strain Population Doubling Calendar Time Human Diploid Fibroblast Young Donor 
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  1. 1.
    Cristofalo, V. J. 1972. Animal cell cultures as a model system for the study of aging. Adv. Gerontol. 4: 45.Google Scholar
  2. 2.
    Hayflick, L. 1965. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614.Google Scholar
  3. 3.
    Hay, R.J. 1970, Cell strain senescence in vitro: -`cell culture anomaly or an expression of a fundamental inability of normal cells to survive and proliferate. In “Aging in Cell and Tissue Culture”, ( E. Holeckova and V.J. Cristofalo, eds.), Plenum Press, New York.Google Scholar
  4. 4.
    Dell’Orco, R.T., Mertens, J.G. and Kruse, P.F. Jr. 1973. Doubling potential, calendar time, and senescence of human diploid cells in culture. Exp. Cell Res. 77: 356.PubMedCrossRefGoogle Scholar
  5. 5.
    Dell’Orco, R.T., Mertens, J.G. and Kruse, P.F. Jr. 1974. Doubling potential, calendar time, and donor age of human diploid cells in culture. Exp. Cell Res. 84: 363Google Scholar
  6. 6.
    Kruse, P.F. Jr., Whittle, W.L. and Miedema, E. 1969. Mitotic and nonmitotic multiple-layered perfusion cultures. J. Cell Biol. 42: 113.PubMedCrossRefGoogle Scholar
  7. 7.
    Tobey, R.A., Crissman,’H.A. and Kraemer, P.M. 1972, A method for comparing effects of different synchronizing protocols on mammalian cell cycle traverse. J. Cell Biol. 54: 638.PubMedCrossRefGoogle Scholar
  8. 8.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem, 193: 265.PubMedGoogle Scholar
  9. 9.
    Dell’Orco, R.T., Crissman, H.A., Steinkamp, J.A. and Kraemer, P.M. Submitted for publication, 1974. Population analysis of arrested human diploid fibroblasts by flow microfluorometry.Google Scholar

Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Robert T. Dell’Orco
    • 1
  1. 1.Biomedical DivisionThe Samuel Roberts Nobel Foundation, Inc.ArdmoreUSA

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