Progesterone Receptors of Chick Oviduct

  • William T. Schrader
  • Bert W. O’Malley
Part of the Advances in Experimental Medicine and Biology book series (AEMB)


The chick oviduct progesterone receptor has been purified to homogeneity by affinity chromatography and its molecular action studied in vitro. The native receptor is a 200,000 MW dimer of two dissimilar 4S subunits with different intranuclear function. The receptors directly regulate RNA chain initiation sites in oviduct chromatin by interactions involving target tissue nuclear acceptor sites. There is a 1:1 correspondence between receptor “acceptor” sites and RNA sites. Only the dimer form of the receptor is active in vitro on chromatin templates. The study suggests a novel model for hormone action which can be tested directly in this system.


Progesterone Receptor Initiation Site Ammonium Sulfate DEAE Cellulose Average Chain Length 
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  1. Bautz, E.K.F. and Bautz, F.A. (1970) Initiation of RNA synthesis:The function of o in the binding of RNA polymerase to promoter sites. Nature 226: 1219–1222.PubMedCrossRefGoogle Scholar
  2. Britten, R.J. and Davidson, E.H. (1969) Gene regulation for higher cells: A theory. Science 165: 349–357.PubMedCrossRefGoogle Scholar
  3. Buller, R.E., Schwartz, R.J., Schrader, W.T., and O’Malley, B.W. (1976) Progesterone-binding components of chick oviduct: In vitro effect of receptor subunits on gene transcription. J. BioZ. Chem. 250: 801–808.Google Scholar
  4. Chan, L., Means, A.R., and O’Malley, B.W. (1973) Rates of induc- tion of specific translatable messenger RNAs for ovalbumin and avidin by steroid hormones. Proc. Nat’l. Acad. Sci. USA 70: 1870–1874.PubMedCrossRefGoogle Scholar
  5. Chamness, G.C. and McGuire, W.L. (1972) Estrogen receptor in the rat uterus. Physiological forms and artifacts. Biochemistry 11: 2466–2472.PubMedCrossRefGoogle Scholar
  6. Comstock, J.P., Rosenfeld, G.C., O’Malley, B.W., and Means, A.R. (1972) Estrogen-induced changes in translation, and specific messenger RNA levels during oviduct differentiation. Proc. Nat’l. Acad. Sci. USA 69: 2377–2380.PubMedCrossRefGoogle Scholar
  7. Cox, R.F. (1973) Transcription of high-molecular-weight RNA from hen-oviduct chromatin by bacterial and endogenous form-B RNA polymerases. Eur. J. Biochem. 39: 49–61PubMedCrossRefGoogle Scholar
  8. Cox, R.F., Haines, M., and Carey, N. (1973) Modification of the template capacity of chick-oviduct chromatin for form-B RNA polymerase by estradiol. Eur. J. Biochem. 32, 513–524.PubMedCrossRefGoogle Scholar
  9. Cuatrecasas, P. and Anfinson, C.B. (1971) Affinity chromatography. Ann. Rev. Biochem. 40: 259–278.PubMedCrossRefGoogle Scholar
  10. Edelman, I.S. and Fimognari, G.M. (1968) I. Modes of hormone action on the biochemical mechanism of action of aldosterone. Rec. Progr. Horm. Res. 24: 1–44.PubMedGoogle Scholar
  11. Erdos, T., Best-Belpomme, M., and Bessada, R. (1970) A rapid assay for binding estradiol to uterine receptor(s). Anal. Biochem. 37: 244–252.PubMedCrossRefGoogle Scholar
  12. Fujii, T., and Villee, C.A. (1968) Effect of testosterone on ribonucleic acid metabolism in the prostate, seminal vesicle, liver and thymus of immature rats. Endocrinology 82: 463–467.PubMedCrossRefGoogle Scholar
  13. Gorski, J. and Nicolette, W. (1963) Early estrogen effects on newly synthesized RNA and phospholipid in subcellular fractions of rat uteri. Arch. Biochem. Biophys. 103: 418–423.PubMedCrossRefGoogle Scholar
  14. Hamilton, T.H., Widnell, C.C., and Tata, J.R. (1965) Sequentialstimulation by oestrogen of nuclear RNA synthesis and DNA-de-pendent RNA polymerase activities in rat uterus. Biochim. Biophys. Acta. 108: 168–172.PubMedCrossRefGoogle Scholar
  15. Harris, S.E., Rosen, J.M., Means, A.R., and O’Malley, B.W. (1975) Use of a specific probe for ovalbumin messenger RNA to quantitate estrogen-induced gene transcripts. Biochemistry 14: 2072–2081.PubMedCrossRefGoogle Scholar
  16. Harris, S.E., Schwartz, R.J., Tsai, M.J., Roy, A.K., and O’Malley, B.W. (1976) Effect of estrogen on gene expression in the chick oviduct: In vitro transcription of the ovalbumin gene in chromatin. J. Biol. Chem. 251: 524–529.PubMedGoogle Scholar
  17. Hartman, H., Nonikel, K.O., Kniisel, F., and Niiesh, J. (1967) The specific inhibition of the DNA-directed RNA synthesis by rifamycin. Biochim. Biophys. Acta. 145: 843–344CrossRefGoogle Scholar
  18. Hinkle, D.C. and Chamberlain, M.J. (1972) Studies of the binding of Escherichia coli RNA polymerase to DNA–I. The role of sigma subunit in site selection. J. Mol. BioZ. 70: 157–185.CrossRefGoogle Scholar
  19. Jaffe, R.C., Socher, S.H., and O’Malley, B.W. (1975) An analysis of the binding of the chick oviduct progesterone-receptor to chromatin. Biochim. Biophys. Acta. 399: 403–419.PubMedCrossRefGoogle Scholar
  20. Jensen, E.V., Mohla, S., Gorell, T.A., and DeSombre, E.R. (1974) The role of estrophilin in estrogen action. Vitamins and Hormones 32: 89–127.PubMedCrossRefGoogle Scholar
  21. Jensen, E.V., Numata, M., Brecher, P.I., and DeSombre, E.R. (1971) Hormone-receptor interaction as a guide to biochemical mechanism. Biochem. Soc. Symp. 32: 133–159.PubMedGoogle Scholar
  22. Kenney, F.T. and Kull, F.J. (1963) Hydrocortisone-stimulated synthesis of nuclear RNA in enzyme induction. Proc. Nat’l. Acad. Sci. USA 50: 493–499PubMedCrossRefGoogle Scholar
  23. Kuhn, R.W., Schrader, W.T., Smith, R.G., and O’Malley, B.W. (1975) Progesterone binding components of chick oviduct–X. Purification by affinity chromatography. J. BioZ. Chem. 250: 4220–4228.Google Scholar
  24. Liarakos, C.D., Rosen, J.M.,and O’Malley, B.W. (1973) Effect of estrogen on gene expression in the chick oviduct–II. Transcription of chick tritiated unique deoxyribonucleic acid as measured by hybridization in ribonucleic acid excess. Biochemistry 12: 2809–2816.Google Scholar
  25. Lill, H., Lill, U., Sippel, A., and Hartmann, G. (1970) In:Lepetit colloquium on RNA polymerase and transcription (Silvestri, L.G., ed.), p. 55, North Holland Publishing Co., Amsterdam.Google Scholar
  26. Lowry, 0.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265.Google Scholar
  27. Mainwaring, W.I.P., Wilce, P.A., and Smith, A.E. (1974) Studies on the form and synthesis of messenger ribonucleic acid in the rat ventral prostate gland, including its tissue-specific stimulation by androgens. Biochem. J. 137: 531–524.Google Scholar
  28. McKnight, S.G., Pennequin, P., and Schimke, R.J. (1975) Induction of ovalbumin mRNA sequences by estrogen and progesterone in chick oviduct as measured by hybridization to complementary DNA. J. Biol. Chem. 250: 8105–8110.PubMedGoogle Scholar
  29. Means, A.R., Comstock, J.P., Rosenfeld, G.C., and O’Malley, B.W. (1972) Ovalbumin messenger RNA of chick oviduct: Partial characterization, estrogen dependence, and translation in vitro. Proc. Nat’l. Acad. Sci. USA 69: 1146–1150.CrossRefGoogle Scholar
  30. Norman, A.W. (1966) Vitamin D mediated synthesis of rapidly labeled RNA from intestinal mucosa. Biochem. Biophys. Res. Conuntac. 23: 335–340.CrossRefGoogle Scholar
  31. Notides, A.C., Hamilton, D.E., and Auer, H.E. (1975) A kinetic analysis of the estrogen receptor transformation. J. Biol. Chem. 250: 3945–3950.PubMedGoogle Scholar
  32. O’Malley, B.W. and McGuire, W.L. (1968) Altered gene expression during differentiation: Population changes in hybridizable RNA after stimulation of the chick oviduct with oestrogen. Nature 218: 1249–1251.PubMedCrossRefGoogle Scholar
  33. O’Malley, B.W., McGuire, W.L., Kohler, P.O.,and Koreman, S.G. (1969) Studies on the mechanism of steroid hormone regulation of synthesis of specific proteins. Recent Progr. Hormone Res. 25: 105–160.PubMedGoogle Scholar
  34. O’Malley, B.W. and Means, A.R. (1974) Female steroid hormones and target cell nuclei. Science 183: 610–620.PubMedCrossRefGoogle Scholar
  35. O’Malley, B.W., Schrader, W.T., and Spelsberg, T.C. (1973) In: Receptors for reproductive hormones (O’Malley, B.W. and Means, A.R., eds.), pp. 174–196, Plenum Publishing Corp., New York.Google Scholar
  36. O’Malley, B.W., Sherman, M.R., Toft, D.O., Spelsberg, T.C., Schrader, W.T., and Steggles, A.W. (1971) A specific oviduct target-tissue receptor for progesterone. Identification, characterization, partial purification, intercompartmental transfer kinetics and specific interaction with the genome. In: Adv. Biosci., Vol. 7 ( Raspé, G., ed.), pp. 213–234, Pergamon Press, New York.Google Scholar
  37. O’Malley, B.W., Spelsberg, T.C., Schrader, W.T., Chytil, F., and Steggles, A.W. (1972) Mechanisms of interaction of a hormone-receptor complex with a genome of a eukaryotic target cell. Nature 235: 141–144.PubMedCrossRefGoogle Scholar
  38. Palmiter, R.D. (1973) Rate of ovalbumin messenger ribonucleic acid synthesis in the oviduct of estrogen-primed chicks. J. Biol. Chem. 248: 6230–8260.Google Scholar
  39. Palmiter, R.D. and Smith, L.T. (1973) Purification and translation of ovalbumin, conalbumin, ovomucoid and lysozyme messenger-RNA. Mol. BioZ. Reports 1: 129–134.CrossRefGoogle Scholar
  40. Rosenfeld, G.C., Comstock, J.P., Means, W.R., and O’Malley, B.W. (1972) Estrogen-induced synthesis of ovalbumin messenger RNA and its translation in a cell-free system. Biochem. Biophys. Res. Commun. 46: 1695–1703.PubMedCrossRefGoogle Scholar
  41. Rosner, W. and Bradlow, H.L. (1971) Purification of corticosteroid-binding globulin from human plasma by affinity chromatography. J. Clin. Endocrinol. Metab. 33: 193–198.PubMedCrossRefGoogle Scholar
  42. Schrader, W.T. (1975) Methods for extraction and quantification of receptors. Methods Enzymol. 36: 187–211.PubMedCrossRefGoogle Scholar
  43. Schrader, W.T., Buller, R.E., Kuhn, R.W., and O’Malley, B.W. (1974) Molecular mechanisms of steroid hormone action. J. Steroid Biochem. 5: 989–999.CrossRefGoogle Scholar
  44. Schrader, W.T., Heuer, S.S., and O’Malley, B.W. (1975) Progesterone receptors of chick oviduct: Identification of 6S receptor dimers. Biol. Reproduct. 12: 134–142.CrossRefGoogle Scholar
  45. Schrader, W.T. and O’Malley, B.W. (1972) Progesterone-binding components of chick oviduct–IV. Characterization of purified subunits. J. Biol. Chem. 247: 51–59.PubMedGoogle Scholar
  46. Schrader, W.T., Toft, D.O., and O’Malley, B.W. (1972) Progesterone-binding protein of chick oviduct–VI. Interaction of purified progesterone-receptor components with nuclear constituents. J. Biol. Chem. 247: 2401–2407.PubMedGoogle Scholar
  47. Schwartz, R.J., Kuhn, R.W., Buller, R.E., Schrader, W.T., andO’Malley, B.W. (1976) Progesterone-binding components of chick oviduct: In vitro effects of purified hormone-receptor complexes on the initiation of RNA synthesis in chromatin. J. Biol. Chem. 251: 5166–5177.PubMedGoogle Scholar
  48. Schwartz, R.J. Tsai, M.J., Tsai, S.Y., and O’Malley, B.W. (1975) Effect of estrogen on gene expression in the chick oviduct–V. Changes in the number of RNA polymerase binding and initiation sites in chromatin. J. Biol. Chem. 250: 5175–5182.Google Scholar
  49. Sekeris, C.E. and Lang, N. (1964) Induction of dopa-decarboxylase activity by insect messenger RNA in an in vitro amino acid incorporating system from rat liver Life Sci. 3:625–632.PubMedCrossRefGoogle Scholar
  50. Sherman, M.R., Corvol, P.L., and O’Malley, B.W. (1970) Progester- one-binding components of chick oviduct–I. Preliminary characterization of cytoplasmic components. J. BioZ. Chem. 245: 6085–6096.Google Scholar
  51. Sica, V., Parikh, I., Nola, E., Puca, G.A., and Cuatrecasas, P. (1973) Affinity chromatography and the purification of estrogen receptors. J. BioZ. Chem. 248: 6543–6558.Google Scholar
  52. Sippel, A.E. and Hartmann, G.R. (1970) Rifampicin resistance of RNA polymerase in the binary complex with DNA. Eur. J. Biochem. 16:152–157.PubMedCrossRefGoogle Scholar
  53. Spelsberg, T.C., Steggles, A.W., Chytil, F., and O’Malley, B.W. (1972) Progesterone-binding components of chick-oviduct–V. Exchange of progesterone-binding capacity from target to non-target tissue chromatins. J. Biol. Chem. 247: 1368–1374.PubMedGoogle Scholar
  54. Spelsberg, T.C., Steggles, A.W., and O’Malley, B.W. (1971) Pro- gesterone-binding components of chick-oviduct–III. Chromatin acceptor sites. J. Biol. Chem. 246: 4188–4197.PubMedGoogle Scholar
  55. Stancel, G.M., Leang, K.M.T., and Gorski, J. (1973) Estrogen receptors in the rat uterus. Relationship between cytoplasmic and nuclear forms of the estrogen binding protein. Biochemistry 12:2137–2141.PubMedCrossRefGoogle Scholar
  56. Stohs, S.J., Zall, J.E., and DeLuca, H.F. (1967) Vitamin D stimu-lation of [3H]orotic acid incorporation into ribonucleic acid of rat intestinal mucosa. Biochemistry 6: 1304–1310.PubMedCrossRefGoogle Scholar
  57. Travers, A., Baillie, D.L., and Pederson, S. (1973) Effect of DNA conformation on ribosomal RNA synthesis in vitro. Nature New Biol. 243:161–163.PubMedCrossRefGoogle Scholar
  58. Tsai, M.J., Schwartz, R.J., Tsai, S.Y., and O’Malley, B.W. (1975) Effects of estrogen on gene expression in the chick oviduct–IV. Initiation of RNA synthesis on DNA and chromatin. J. Biol. Chem. 250: 5165–5174.PubMedGoogle Scholar
  59. Umezawa, H., Mizuno, S., Uamasaki, H., and Nitta, K. (1968) Inhib-ition of DNA-dependent RNA synthesis by rifamycins. J. Antibiotics 21: 234–236.CrossRefGoogle Scholar
  60. Zillig, W., Zechel, D., Rabussay, D., Schachne, M., Sethi, V.S., Palm, P., Heil, A., and Seifert, W. (1970) On the role of different subunits of DNA-dependent RNA polymerase from E. coli in the transcription process Cold Spring Harbor Symp. Quant. BioZ. 35:47–58.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • William T. Schrader
    • 1
  • Bert W. O’Malley
    • 1
  1. 1.Department of Cell BiologyBaylor College of MedicineHoustonUSA

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