Further Characterization and Applications of the 4-Chlorobiphenyl Hydroxylase Assay

  • A. Parkinson
  • L. W. Robertson
  • S. Safe
Part of the Advances in Experimental Medicine and Biology book series (AEMB)


Studies of the metabolism-mediated covalent binding of halobiphenyls to cellular macromolecules revealed that the in vitro metabolism of 4-chlorobiphenyl (4-CBP) by hepatic microsomes is greatly enhanced by in vivo pretreatment of rats with 3-methylcholanthrene (MC) but not phenobarbitone (PB) (Crawford and Safe, 1979). This differential response enables these two classes of inducers to be readily distinguished (Parkinson et al., 1980a).


Hepatic Microsome Polycyclic Hydrocarbon Aryl Hydrocarbon Hydroxylase Microsomal Electron Transport Hepatic Microsome Enzyme Activity 
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Copyright information

© Springer Science+Business Media New York 1982

Authors and Affiliations

  • A. Parkinson
    • 1
  • L. W. Robertson
    • 1
  • S. Safe
    • 1
  1. 1.Guelph-Waterloo Centre for Graduate Work in Chemistry Department of ChemistryUniversity of Guelph GuelphOntarioCanada

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