Development of a Novel Method for Measuring Covalent Binding and Its Application to Investigations of Bromobenzene Hepatotoxicity

  • John G. Dent
  • James D. Sun
Part of the Advances in Experimental Medicine and Biology book series (AEMB)


The covalent binding of chemicals or metabolites to cellular tissue macromolecules may represent an important mechanism for chemical toxicity. Covalent interactions have been suggested to be involved in hepatic, renal, pulmonary and bone marrow toxicity as well as carcinogenicity (Mitchell et al., 1976; Mitchell et al., 1977; Hinson, 1980; Gillette et al., 1974; Boyd, 1980; Irons et al., 1980; Miller, 1970). More recently it has become apparent that the amount of covalent binding measured in a biological system may not be the most toxicologically relevant parameter. Gillette (1974) emphasized that reactive metabolites may bind to noncritical macromolecules and such interaction may be of little or no functional significance. To further our understanding of the role of covalent binding in chemical toxicity it is necessary to study the specificity of covalent binding.


Covalent Binding Covalent Interaction High Molecular Weight Fraction Hepatocyte Incubation Microsomal Mixed Function Oxidase 
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Copyright information

© Springer Science+Business Media New York 1982

Authors and Affiliations

  • John G. Dent
    • 1
  • James D. Sun
    • 1
  1. 1.Chemical Industry Institute of ToxicologyResearch Triangle ParkUSA

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