Heart Model Supported in Organ Culture and Analyzed by Acoustic Microscopy

  • Reginald C. Eggleton
  • F. Stephen Vinson


Various cardiac models have been described in the literature which have been used extensively in the study of the physiology, biochemistry and pharmacology of the heart. Since the original work of Langendorff in 18981 there have been numerous improvements in cardiac models. Bleehen and Fisher2 simplified the preparation by adding recirculation. In the original cardiac model, no ventricular filling occurred; thus little work was done by the left ventricle, i.e., work done in contraction is a function of the initial length of the fibers. To correct this deficiency, Morgan et al.3 added loading to the heart. Opie4 in England and Arnold and Lochner5 on the continent prepared an isovolumic constant volume, and later, Neely (1967)6 and Rabitzsch (1968)7 improved on the working heart model. Since that time various other improvements have been added.8–10


Organ Culture Transit Time Heart Model Acoustic Image Acoustic Microscopy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Langendorff, O.: Untersuchungen am überlebenden Saugetierherzen Pflügers Arch. ges. Physiol., 61, 291 (1895). Google Scholar
  2. 2.
    Bleehen, N. M. and Fisher, R. The action of insulin on the isolated rat heart. J. Physio Load., 123, 260 (1954). Google Scholar
  3. 3.
    Morgan, H. E., Henderson, M. J.,,Regen, D. M. and Park, C. R.:Google Scholar
  4. Regulation of glucose uptake in muscle. I. The effect of insu-lin and anoxia on glucose transport and isolated, perfused heart of normal rats. J. boil. Chem. 236, 253, (1961).Google Scholar
  5. 4.
    Opie, L. H.: Coronary flow rate and perfusion pressure as deter-minants of mechanical function and oxidative metabolism of isolated perfused rat heart, Physiol., Lend., 180, 529 (1965),Google Scholar
  6. 5.
    Arnold, G. and Lochner, W.: Die Temperaturabhangigkeit des Sauerstoffverbrauches stillgestellter, künstlich perfundierter Warmblüterherzen zwischen 34° und 4°C. Pflügers Arch. ges, Physiol., 284, 169 (1965). Google Scholar
  7. 6.
    Neely, J. R., Liebermeister, H., Battersby, E. J. and Morgan, H. E.: Effect of pressure development on oxygen consumption by isolated rat heart. Am. J. Physiol., 212, 804 (1967). Google Scholar
  8. 7.
    Rabitzsch, G,: Koronarrperfusion isolierter Warmblütterherzen mit geringen Umlaufsvolumina und Kontinuierlicher Kontractions- und koronarflußregistrierung. 33 (1958).Google Scholar
  9. 8.
    Wildenthal, K: Long-term maintenance of spontaneously beating mouse hearts in organ culture. J. Appl. Physiol., 30 (1), 153 (1971).Google Scholar
  10. 9.
    Opie, L. H., Mansford, K. R. L. and Owen, P. Effects of in-creased heart work on glycolysis and adenine nucleotides in the perfused heart of normal and diabetic rats. Blochern. J., 124, 475 (1971). Google Scholar
  11. 10.
    Neely, J. R., Denton, R. M., England, P. J. and Randle, P. J.: The affects of increased heart work on the tricarboxylate cycle and its interactions with glycolysis in the perfused rat heart. Biochem. J. 128, 147 (1972).Google Scholar
  12. 11.
    Eggleton, R. C. and Kessler, L. W.: Mouse embryo heart in organ culture visualized by the acoustic microscope. In Ultrasound in Medicine , Vol 1 , ed. D. White (Plenum Publ. Corp., New York 1975) pp. 537-542. (continued)Google Scholar
  13. 12.
    Eggleton, R. C., Kessler, L. W., Vinson, F. S. and Bader, G. B. Acoustic microscopy of live mouse embryo hearts. In 1975 Ultrasonics Symposium Proceedings (IEEE, Inc., New York 1975), pp. 57-58. Google Scholar
  14. 13.
    Eggleton, R. C., Kessler, L. W., Vinson, F. S. and Boder, G. B.: Effect of drugs on mouse embryo, hearts in organ culture visualized by acoustic microscopy. In Ultrasound in Medicine , Vol. 2 , eds. D. White and R. Barnes (Plenum Pub1. Corp., New York 1976), pp. 441-443.Google Scholar
  15. 14.
    Vinson, F. S.,-Eggleton,R, C.,,Boder, G. B. and Kessler, L. W.: Measurement of time related cardiac parameters by acoustic micro- scopy. Presented at WFUMB ‘76 Scientific Sessions, San Francisco, August 1-7, 1976.Google Scholar
  16. 15.
    Korpel, A., Kessler, L. scope operating at 100 July (1971). Sonomicros 720, Foster Avenue Bens W. and Palermo, P. R.: Acoustic micro- MHz. Nature 232, No. 5306, pp. 110-111,cope 100 manufactured by Sonoscan, Inc. enville,;Ii. 60106.Google Scholar
  17. 16.
    Fung, Y. C.: Comparison of different models of the heart muscle. J.Biomechanics 4, 289 (1971) Google Scholar

Copyright information

© Springer Science+Business Media New York 1977

Authors and Affiliations

  • Reginald C. Eggleton
    • 1
  • F. Stephen Vinson
    • 1
  1. 1.Indiana University School of Medicine, and Fortune-Fry Research Laboratories of thee Indianapolis Center for Advanced ResearchIndianapolisUSA

Personalised recommendations