Ion-Exchange Chromatography of Bile Acids

  • A. Kuksis

Abstract

The common bile acids and their glycine and taurine conjugates are relatively freely soluble in aqueous solutions and possess dissociation constants that range from near neutrality to values exhibited by the strongest of organic acids. Ion-exchange chromatography should therefore be theoretically suitable for an efficient separation of such molecules. In practice, however, the adaptation of ion-exchange techniques to bile acid work has been limited and progress extremely slow. This has been partly due to the great success of the thin-layer and gas-liquid chromatographic techniques as means of analysis of bile acids, as well as to the discovery of extremely selective solvent pairs for the separate extraction of the glycine and taurine conjugates from natural sources. The main difficulty has been the complex behavior of these natural detergents in aqueous solutions. The realized separations apparently have involved certain sorbent properties of the ion exchangers as well as chromatography of ions by ion exchange.

Keywords

Bile Acid Lithocholic Acid Bile Acid Conjugate Fecal Bile Acid Glycine Conjugate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1971

Authors and Affiliations

  • A. Kuksis
    • 1
  1. 1.Banting and Best Department of Medical ResearchUniversity of TorontoTorontoCanada

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