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Extraction, Purification, and Chromatographic Analysis of Bile Acids in Biological Materials

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The Bile Acids Chemistry, Physiology, and Metabolism

Abstract

Several reviews covering different aspects of bile acid analysis have appeared in recent years (1–15). These articles cover a wide range of techniques with particular emphasis on thin-layer and gas chromatography. In this chapter we will try to present established methods for bile acid analysis as they can be combined to form a complete analytical procedure, including extraction, purification, identification, and quantitation. It has not been possible to include all methods and the various modifications of standard procedures that have been published. References to most of these can be found in the reviews (1–15). Early development in the field is described in Sobotka’s books on bile acids and steroids (16, 17). Whenever equally efficient or similar methods exist we have generally preferred to describe those of which we have personal experience. Many of the techniques used for bile acids are similar to those used in steroid analysis and advance in this area is highly relevant for workers in the bile acid field.

Supported by grants from the Swedish Medical Research Council (13x-219 and 13x-2520).

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  1. Department of Chemistry, Karolinska Institutet, Stockholm, Sweden

    P. Eneroth & J. Sjövall

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  1. P. Eneroth
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  1. Biochemistry Research Division Department of Medicine, Sinai Hospital of Baltimore, Inc., Baltimore, Maryland, USA

    Padmanabhan P. Nair

  2. The Wistar Institute, Philadelphia, Pennsylvania, USA

    David Kritchevsky

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Eneroth, P., Sjövall, J. (1971). Extraction, Purification, and Chromatographic Analysis of Bile Acids in Biological Materials. In: Nair, P.P., Kritchevsky, D. (eds) The Bile Acids Chemistry, Physiology, and Metabolism. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0647-5_5

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