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Effects of Aldehyde Carbon Chain Length and Type of Luciferase on the Quantum Yields of Bacterial Bioluminescence

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Book cover Chemiluminescence and Bioluminescence

Abstract

Bacterial bioluminescence results from the oxidation of reduced flavin mononucleotide (FMNH2) and an aliphatic aldehyde (RCHO) by molecular oxygen in the presence of the enzyme bacterial luciferase. The final product of the reaction is FMN but as shown in Figure I, perturbation of its fluorescence, for instance by an environment of low polarity, cannot blue shift its fluorescence to overlap the spectral emission distribution of the bioluminescence. In addition the low polarity enhances a vibrational structure in the FMN fluorescence whereas the properly corrected bioluminescence is unstructured. Lumiflavin is used here since FMN is insoluble in solvents of low polarity.

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Reference

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Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Georgia, Athens, Georgia, 30601, USA

    John Lee & Charles L. Murphy

Authors
  1. John Lee
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  2. Charles L. Murphy
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Editor information

Editors and Affiliations

  1. Department of Biochemistry and Chemistry, University of Georgia, Athens, Georgia, USA

    Milton J. Cormier , David M. Hercules  & John Lee ,  & 

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© 1973 Springer Science+Business Media New York

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Lee, J., Murphy, C.L. (1973). Effects of Aldehyde Carbon Chain Length and Type of Luciferase on the Quantum Yields of Bacterial Bioluminescence. In: Cormier, M.J., Hercules, D.M., Lee, J. (eds) Chemiluminescence and Bioluminescence. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0638-3_28

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  • DOI: https://doi.org/10.1007/978-1-4757-0638-3_28

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0640-6

  • Online ISBN: 978-1-4757-0638-3

  • eBook Packages: Springer Book Archive

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