Bacterial Bioluminescence. Mechanistic Implications of Active Center Chemistry of Luciferase

  • J. W. Hastings
  • A. Eberhard
  • T. O. Baldwin
  • M. Z. Nicoli
  • T. W. Cline
  • K. H. Nealson

Abstract

Viewed as a chemiluminescent reaction, the enzyme luciferase plays a truly important role with regard to the reaction pathway. The non-enzymatic oxidation of reduced flavin mononucleotide (FMNH2) has a very low quantum yield: less than 10−10 of the photons which can be evoked enzymatically are observed in the non-enzymatic oxidation of FMNH2 in the presence of a long chain aldehyde.

Keywords

Fluorescence Emission Spectrum Bacterial Luciferase Cysteinyl Residue Chain Aldehyde Bacterial Bioluminescence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Gibson, Q. H., and Hastings, J. W., Biochem. J., 83, 368 (1962).Google Scholar
  2. 2.
    Massey, V., Palmer, G., and Ballou, D., in “Flavins and Flavoproteins,” (H. Kamin, ed.), p. 349, University Park Press, Baltimore, Md. (1971).Google Scholar
  3. 3.
    Mc Elroy, W. D., et al., Arch. Biochem. Biophys. 56, 240 (1955).CrossRefGoogle Scholar
  4. 4.
    Nealson, K. H., et al., J. Biol. Chem., 247, 888 (1972).Google Scholar
  5. 5.
    Eberhard, A., and Hastings, J. W., Biochem. Biophys. Res. Comm., 47, 348 (1972).Google Scholar
  6. 6.
    Hastings, J. W., et al., J. Biol. Chem., 238, 2537 (1963).Google Scholar
  7. 7.
    Friedland, J. M., and Hastings, J. W., PNAS, 58, 2336 (1967).Google Scholar
  8. 8.
    Gunsalus-Miguel, A., et al., J. Biol. Chem., 247, 398 (1972).Google Scholar
  9. 9.
    Meighen, E. A., et. al., J. Biol. Chem., 246, 7666 (1971).Google Scholar
  10. 10.
    Meighen, E. A., Nicoli, M. Z., and Hastings, J. W., Biochemistry, 10, 4062, 4069 (1971).CrossRefGoogle Scholar
  11. 11.
    Cline, T. W., and Hastings, J. W., Biochemistry 11, 3359 (1972).CrossRefGoogle Scholar
  12. 12.
    Cline, T. W., PhD thesis, Harvard University, 1973.Google Scholar
  13. 13.
    Nicoli, M. Z., PhD thesis, Harvard University, 1973.Google Scholar
  14. 14.
    Baldwin, T. 0., and Nicoli, M. Z., manuscript in preparation.Google Scholar
  15. 15.
    Mitchell, G. W., and Hastings, J. W., J. Biol. Chem., 244, 2572 (1969).Google Scholar
  16. 16.
    Eley, M., Lee, J., Lhoste. J.-M., Cormier, M. J., and Hemmerich, P., Biochemistry, 9, 2902 (1970).CrossRefGoogle Scholar
  17. 17.
    Hastings, J. W., et al., Biochemistry, 8, 4681 (1969).CrossRefGoogle Scholar
  18. 18.
    Hastings, J. W., Gibson, Q. H., Friedland, J., and Spudich, J., in “Bioluminescence in Progress,” (F. H. Johnson and Y. Haneda, eds.), p. 151, Princeton University Press (1966).Google Scholar
  19. 19.
    Shimomura, 0., Johnson, F. H., and Kohama, Y., PNAS, 69, 2086 (1972).CrossRefGoogle Scholar
  20. 20.
    Nakamura, T. and Matsuda, K., J. Biochem., 70, 35 (1971).Google Scholar
  21. 21.
    Lee, J., Biochemistry, 11, 3350 (1972).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1973

Authors and Affiliations

  • J. W. Hastings
    • 1
  • A. Eberhard
    • 1
  • T. O. Baldwin
    • 1
  • M. Z. Nicoli
    • 1
  • T. W. Cline
    • 1
  • K. H. Nealson
    • 1
  1. 1.Harvard University, Ithaca Collegeand the University of MassachusettsUSA

Personalised recommendations