Ethanol-Induced Changes in Cation-Stimulated Adenosine Triphospha-Tase Activity and Lipid-Proteolipid Labeling of Brain Microsomes

  • Henrik Wallgren
  • Pirkko Nikander
  • Pekka Virtanen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 59)


Our working hypothesis was presented at the previous ICAA symposium on alcohol intoxication and withdrawal in Amsterdam 1972 (Wallgren, 1973). We assume that prolongation of the depressant action of alcohol causes compensatory changes particularly in those neuronal membrane structures which are involved in control of the excitation cycle of the conducting membranes. Adaptive changes in the synaptic region are presumably also involved and actually form an important focussing point for current alcohol research.


ATPase Activity Microsomal Fraction Alcohol Withdrawal Syndrome Ethanol Dose Brain Microsome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Akera, T., Rech, R.H., Marquis, W.J., Tobin, T., and Brody, T.M., 1973. Lack of relationship between brain (Nat, K+)-activated adenosine triphosphatase and the development of tolerance to ethanol in rats, J. Pharmacol. Exp. Ther. 185: 594–601.Google Scholar
  2. Arvola, A., Sammalisto, L., and Wallgren, H., 1958. A test for level of alcohol intoxication in the rat, Quart. J. Stud. Alc. 19: 563–572.Google Scholar
  3. Bowler, K. and Duncan, C.J., 1968. Effect of temperature on the Mgt+ dependent and Na+-K+ATPases of a rat brain microsomal preparation, Comp. Biochem. Physiol. 24: 1043–1054.Google Scholar
  4. Cicero, T.J., Snider, S.R., Perez, V.J., and Swanson, L.W., 1971. Physical dependence on and tolerance to alcohol in the rat, Physiol. Behay. 6: 191–198.Google Scholar
  5. Falk, J.L., Samson, H.H., and Winger, G., 1972. Behavioral maintenance of high concentrations of blood ethanol and physical dependence in the rat, Science 177: 811–813.CrossRefGoogle Scholar
  6. Folch, J., Ascoli, I., Lees, M., Meath, J.A., and LeBaron, F.N., 1951. Preparation of lipide extracts from brain tissue, J. Biol. Chem. 191: 833–841.Google Scholar
  7. Folch, J. and Lees, M., 1951. Proteolipides, a new type of tissue lipoproteins, J. Biol. Chem. 191: 807–817.Google Scholar
  8. Goldstein, D.B., 1972. Relation of alcohol dose intensity of Withdrawal signs in mice, J. Pharmacol. Exp. Ther. 180: 203–215.Google Scholar
  9. Goldstein, D.B. and Israel, Y., 1972. Effects of ethanol on mouse brain (Nat, K+)-activated adenosine triphosphatase, Life Sci. 11: Part II; 957–963.Google Scholar
  10. Israel, Y., Kalant, H., LeBlanc, A.E., Bernstein, J.C., and Salazar, J., 1970. Changes in cation transport and (Na+, K+)-activated adenosine triphosphatase produced by chronic administration of ethanol, J. Pharmacol. Exp. Ther. 174: 330–336.Google Scholar
  11. Israel, Y. and Kuriyama, K., 1971. Effects of in vivo ethanol administration on adenosine triphosphatase activity of sub-cellular fractions of mouse brain and liver, Life Sci. 10: 591–599.CrossRefGoogle Scholar
  12. Knox, W.H., Perrin, R.G., and Sen, A.K., 1972. Effect of chronic administration of ethanol on (Na+, K+)-activated ATPase activity in six areas of the cat brain, J. Neurochem. 19: 2881–2884.CrossRefGoogle Scholar
  13. Lieber, C.S. and De Carli, L.M., 1973. Ethanol dependence and tolerance: a nutritionally controlled experimental model in the rat, Res. Commun. Chem. Pathol. Pharmacol. 6: 983–991.Google Scholar
  14. Nikander, P., 1972. Carbon electrodes for stimulation of brain tissue in vitro J. Neurochem. 19: 535–537.CrossRefGoogle Scholar
  15. Peacock, A.J., Bowler, K., and Anstee, J.H., 1972. Demonstration of a Na+-K+-Mg2+ dependent ATPase in a preparation from hindgut and Malpighian tubules of two species of insect, Experientia 28: 901–902.CrossRefGoogle Scholar
  16. Roach, M.K., Khan, M.M., Coffman, R., Pennington, W., and Davis, D.L., 1973. Brain (Na+,K+)-activated adenosine triphosphatase activity and neurotransmitter uptake in alcohol-dependent rats, Brain Res. 63: 323–329.CrossRefGoogle Scholar
  17. Wallgren, H., 1973. Neurochemical aspects of tolerance to and de-pendence on ethanol, in Alcohol Intoxication and Withdrawal, Experimental Studies I, ( M.M. Gross, ed.), pp. 15–31, Plenum Press, New York.CrossRefGoogle Scholar
  18. Wallgren, H., Ahlqvist, J., Âhman, K., and Suomalainen, H., 1967. Repeated alcoholic intoxication compared with continued consumption of dilute ethanol in experiments with rats on a marginal diet, Brit. J. Nutr. 21: 643–660.Google Scholar
  19. Wallgren, H. and Forsander, 0., 1963. Effect of adaptation to alcohol and of age on voluntary consumption of alcohol by rats, Brit.J. Nutr. 17: 453–457.Google Scholar
  20. Wallgren, H., Kosunen, A.-L., and Ahtee, L., 1972. Technique for producing an alcohol withdrawal syndrome in rats, Brain Res. 42: 550.Google Scholar
  21. Wallgren, H., Kosunen, A.-L., and Ahtee, L., 1973. Technique for producing an alcohol withdrawal syndrome in rats, Isr. J. Med. Sci. 9: Suppl. 63–71.Google Scholar
  22. Wallgren, H., Nikander, P., von Boguslawsky, P., and Linkola, J., 1974. Effects of ethanol, tert.butanol and clomethiazole on net movements of sodium and potassium in electrically stimulated cerebral tissue, Acta Physiol. Scand. 91: 83–93.Google Scholar

Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Henrik Wallgren
    • 1
  • Pirkko Nikander
    • 1
  • Pekka Virtanen
    • 1
  1. 1.Research Laboratories of the State Alcohol Monopoly (Alko), and Department of Physiological ZoologyUniversity of HelsinkiHelsinkiFinland

Personalised recommendations