Special Techniques for Weighing Microgram Quantities of Tissue and Assaying Them for Enzyme Activities

  • Charles L. Rutherford


To carry out biochemical analyses on single hydra or parts of an animal and to relate those measurements to the dry weight of the tissue analyzed. There are three essential steps: (1) the quick freeze-drying of the hydra; (2) the dissection and weighing of pieces of dry hydra tissue as light as 0.1 μg; (3) the biochemical assay of those dried tissues.


Copper Wire Rubber Band Ocular Micrometer Pyridine Nucleotide Quartz Fiber 
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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Charles L. Rutherford
    • 1
  1. 1.Biology DepartmentVirginia Polytechnic Institute and State UniversityBlacksburgUSA

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