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Preparing Ectoderm/Endoderm Chimeras

  • Nancy Wanek

Abstract

To produce chimeric hydra consisting of ectoderm from one strain and endoderm from a different strain. The procedure involves vital staining, grafting, and microsurgery. Nitrogen mustard-treated host animals are used to support the growth of small chimeras.

Keywords

Host Animal Nitrogen Mustard Fish Line Glass Micropipet Vital Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Campbell, R. D. 1974. Cell movements in Hydra. Am, Zool, 14:523–535Google Scholar
  2. Macklin, M. 1976. The effect of urethan on hydra. Biol. Bull. 150:442–452Google Scholar
  3. Wanek, N. and Campbell, R D. 1982. Roles of ectodermal and endodermal epithelial cells in hydra rnorphogenesis: construction of chimeric strains.J. Exp. Zool. 221:37–47.Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Nancy Wanek
    • 1
  1. 1.Developmental Biology Center and Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

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