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Separating Viable Tissue Layers

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Hydra: Research Methods
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Abstract

Each of the two tissue layers of hydra possesses a unique and characteristic cellular composition. Therefore, viable separate layers can be used in a variety of physiological and developmental studies. I describe here three commonly used methods for isolating viable hydra endoderm and ectoderm.

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References

  • Burnett, A. L., Davis, L. E., and Ruffing, F. E. 1966. A histological and ultrastructural study of germinal differentiation of interstitial cells arising from gland cells in Hydra viridis. J. Morphol. 120: 1–8.

    Article  CAS  Google Scholar 

  • Burnett, A. L., Lowell, R. D., and Cyrlin, M. 1973. Regeneration of a complete Hydra from a single, differentiated somatic cell type, In: Biology of Hydra ( A. L. Burnett, ed.) Academic Press, New York, pp. 255–267.

    Google Scholar 

  • Davis, L. E. 1970a. Cell division during dedifferentiation and redifferentiation in the regenerating isolated gastrodermis of Hydra. Exp. Cell Res. 60: 217–132.

    Google Scholar 

  • Davis, L. E. 1970b. Further observations on dividing and nondividing cnïdoblasts in the nerating isolated gastrodermis of Hydra. Z. Zellforsch. 105: 526–537.

    Article  CAS  Google Scholar 

  • Davis, L. E., Burnett, A. L., Haynes, J. F., and Mumaw, V. R. 1966. A histological and ultrastructural study of dedifferentiation and redifferentiation of digestive and gland cells in Hydra viridis. Dev. Biol. 14: 307–329.

    Article  Google Scholar 

  • Haynes, J. F., and Burnett, A. L. 1963. Dedifferentiation and redifferentiation of cells in Hydra viridis. Science 142: 1481–1483.

    CAS  Google Scholar 

  • Lowell, R. D., and Burnett, A. L. 1969. Regeneration of complete hydra from isolated epidermal fragments. Biol. Bull. 137: 312–320.

    Article  Google Scholar 

  • Lowell, R. D., and Burnett, A. L. 1973. Regeneration from isolated epidermal explants, In: Biology of Hvdra ( A. L. Burnett, ed.) Academic Press, New York, pp. 223–232.

    Google Scholar 

  • Muscatine, L. 1961. Symbiosis in marine and fresh water coelenterates, In: The Biology of Hydra ( H. M. Lenhoff and W. F. Loomis, eds.), University of Miami Press, Coral Gables, Ha., pp. 255–268.

    Google Scholar 

  • Muscatine, L., and Lenhoff, H. M. 1963. Symbiosis: On the role of algae symbiotic with hydra. Science 142: 956–958.

    Article  PubMed  CAS  Google Scholar 

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© 1983 Springer Science+Business Media New York

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Lesh-Laurie, G.E. (1983). Separating Viable Tissue Layers. In: Lenhoff, H.M. (eds) Hydra: Research Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0596-6_35

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  • DOI: https://doi.org/10.1007/978-1-4757-0596-6_35

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0598-0

  • Online ISBN: 978-1-4757-0596-6

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