Abstract
To investigate the growth and differentiation of interstitial stem cells of hydra, we have developed a method for culturing those cells within aggregates of cells taken from hydra previously treated with nitrogen mustard (NM) (David and Murphy, 1977; Sproull and David, 1979).
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References
David, C. N., and Murphy, S. 1977. Characterization of interstitial stem cells in hydra by cloning. Dev. Biol. 58: 372–383.
David, C. N., and Plotnick, I. 1980. Distribution of interstitial stem cells in Hydra. Dev. Biol. 76: 175–184.
Diehl, F., and Burnett, A. L. 1964. The role of interstitial cells in the maintenance of hydra. 1. Specific destruction of interstititial cells in normal, sexual and non-budding animals./. Exp. Zoo1.155:253–259.
Fujisawa, T., and David, C. N. 1981. Commitment during nematocyte differentiation in hydra. J. Cell Sci. 48:207–222
Gierer, A., Berking, S., Bode, H., David, C. N., Flick, K., Hansmann, G., Schaller, C., and Trenker, E. 1972. Regeneration of hydra from reaggregated cells. Nature (London) New Biol. 239: 98–101.
Sproull, R, and David, C. N. 1979. Stem cell growth and differentiation in Hydra attenuata. I, Regulation of self-renewal probability in multi-clone aggregates. J. Cell Sei. 38. 155–169.
Venugopal, G., and David, C. N. 1981. Nerve commitment in hydra. I. Role of morphogenetic signals. Der. Biol. 83: 353–360.
Yaross, M., Baca, B., and Bode, H. 1982. Commitment of hydra interstitial cells to nerve cell differentiation must be initiated during S-phase. Der. Biol. (in press).
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© 1983 Springer Science+Business Media New York
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David, C.N. (1983). Culturing Interstitial Stem Cells in Hydra Aggregates. In: Lenhoff, H.M. (eds) Hydra: Research Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0596-6_34
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DOI: https://doi.org/10.1007/978-1-4757-0596-6_34
Publisher Name: Springer, Boston, MA
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