Culturing Interstitial Stem Cells in Hydra Aggregates

  • Charles N. David


To investigate the growth and differentiation of interstitial stem cells of hydra, we have developed a method for culturing those cells within aggregates of cells taken from hydra previously treated with nitrogen mustard (NM) (David and Murphy, 1977; Sproull and David, 1979).


Interstitial Cell Clone Growth Nitrogen Mustard Cloning Assay Stem Cell Clone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. David, C. N., and Murphy, S. 1977. Characterization of interstitial stem cells in hydra by cloning. Dev. Biol. 58: 372–383.PubMedCrossRefGoogle Scholar
  2. David, C. N., and Plotnick, I. 1980. Distribution of interstitial stem cells in Hydra. Dev. Biol. 76: 175–184.CrossRefGoogle Scholar
  3. Diehl, F., and Burnett, A. L. 1964. The role of interstitial cells in the maintenance of hydra. 1. Specific destruction of interstititial cells in normal, sexual and non-budding animals./. Exp. Zoo1.155:253–259.Google Scholar
  4. Fujisawa, T., and David, C. N. 1981. Commitment during nematocyte differentiation in hydra. J. Cell Sci. 48:207–222Google Scholar
  5. Gierer, A., Berking, S., Bode, H., David, C. N., Flick, K., Hansmann, G., Schaller, C., and Trenker, E. 1972. Regeneration of hydra from reaggregated cells. Nature (London) New Biol. 239: 98–101.CrossRefGoogle Scholar
  6. Sproull, R, and David, C. N. 1979. Stem cell growth and differentiation in Hydra attenuata. I, Regulation of self-renewal probability in multi-clone aggregates. J. Cell Sei. 38. 155–169.Google Scholar
  7. Venugopal, G., and David, C. N. 1981. Nerve commitment in hydra. I. Role of morphogenetic signals. Der. Biol. 83: 353–360.Google Scholar
  8. Yaross, M., Baca, B., and Bode, H. 1982. Commitment of hydra interstitial cells to nerve cell differentiation must be initiated during S-phase. Der. Biol. (in press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Charles N. David
    • 1
  1. 1.Department of Molecular BiologyAlbert Einstein College of MedicineBronxUSA

Personalised recommendations