Dissociating Tissues into Cells and the Development of Hydra from Aggregated Cells

  • Kristine M. Flick
  • Hans R. Bode


To dissociate hydra tissue into a suspension of viable cells, and to form from those cells aggregates of tissue that will develop into viable whole hydra.


Hollow Sphere Culture Solution Microfuge Tube Nylon Cloth Large Clump 
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  1. Campbell, R. D. 1976. Elimination of Hydra interstitial and nerve cells by means of colchicine. J. Cell Sci. 21: 1–13.PubMedGoogle Scholar
  2. David, C. D., and Murphy, S. 1977. Characterization of interstitial stem cells in Hydra by cloning. Del,. Biol. 58: 372–383.Google Scholar
  3. Diehl, F. A., and Burnett, A. L. 1964. The role of interstitial cells in the maintenance of Hydra. I. Specific destruction of interstitial cells to normal, asexual, and non-budding animals. J. Exp. Zool. 155: 253–259.PubMedCrossRefGoogle Scholar
  4. Gierer, A., Berking, S., Bode, H., David, C. N., Flick, K., Hansmann, G., Schaller, H., and Trenkner, E. 1972. Regeneration of Hydra from reaggregated cells. Nature (London) New Biol. 239: 98–101.CrossRefGoogle Scholar
  5. Trenkner, E., Flick, K., Hansmann, G., Bode, P., and Bode, H. 1973. Studies on Hydra cells in vitro. J. Exp. Zool. 185: 317–326.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Kristine M. Flick
    • 1
  • Hans R. Bode
    • 1
  1. 1.Developmental Biology Center and Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

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