Preparing Histological Sections for Light Microscopy

  • Richard D. Campbell


To section hydra that have been embedded in paraffin for histological analysis with the light microscope.


Toluidine Blue Methyl Salicylate Aniline Blue Fast Green Acid Fuchsin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Benthin, M. G., and Davis, L. E. 1976. The qualitative use of Cason’s one-step triple stain on Hydra. Cytologia 41:741–748Google Scholar
  2. Bouillon, J. 1966. Les cellules glandulaires des hydroides et hydrorné Leur structure et la nature de leurs sécrétions, Cal:. Biol. Mar. 7: 157–205.Google Scholar
  3. Bouillon, J. and Coppois. G. 1977. Etude comparative de la rnesoglee des Cnidaires. Cah. Biol, Mar. 18: 339–368.Google Scholar
  4. Burnett, A. L. 1959. Histophysiology of growth in hydra..), Exp. ZooL 140, 281–341.CrossRefGoogle Scholar
  5. Campbell, R. D. 1967. Tissue dynamics of steady state growth in Hydra littoralis. I. Patterns of cell division. Dev. Biol. 15: 487–502.PubMedCrossRefGoogle Scholar
  6. Campbell, R. D. 1981. A method for making ribbons of semithin plastic sections, Stain Technol. 56: 247–249.PubMedGoogle Scholar
  7. Davis, L. E., Burnett, A. L., Haynes, J. F., and Mumaw, V. R. 1966, A histological and ultrastructural study of dedifferentiation and redifferentiation of digestive and gland cells in Hydra viridis, Dev. Biol. 14: 307–329.CrossRefGoogle Scholar
  8. Diehl, F. A,, and Burnett, A. L. 1964. The role of interstitial cells in the maintenance of Hydra. I. specific destruction of interstitial cells in normal, asexual, non-budding, animals. J. Exp. Zool. 155: 253–260.Google Scholar
  9. Gurr, E. 1958. Methods of Analytical Histology and Histochemistry, Leonard Hill Ltd., London.Google Scholar
  10. Gurr, E. 1962, Staining, Practical and Theoretical. Williams Sc Wilkins, Baltimore, Md. Hadii, J. 1909. Ueber das Nervensystem von Hydra. Arh. Zool. Inst. Univ, Wien 17: 225–268.Google Scholar
  11. Holmes, W. 1950. The mesoglea and muscle-fibres of Chlorohydra viridissima. Q. J, Microscop. Sci. 91: 419–428.Google Scholar
  12. Humason, G. L. 1967. Animal Tissue Techniques, 2nd ed, Freeman, San Francisco. Kanajew, J. 1930. Zur Frage der Bedeutung der Interstitiellen Zellen bei Hydra. Wilhelm Roux Arch. Entwicklungsmech. Org. 122: 736–759.Google Scholar
  13. Macklin, M. 1976. The effect of urethan on hydra. Biol. Bull. 150: 442–452.PubMedCrossRefGoogle Scholar
  14. McConnell, C. H. 1932. Zellteilungen bei Hydra. Mitosen in den epithelio-muskulaeren Zellen des Entoderms von Hydra. Z. Mikrosk, Anat. Forsch, 28:578–588Google Scholar
  15. McConnell, C. H. 1933. Mitosis in hydra: Mitosis of the secretory cells of the endoderm of hydra. Biol. Bull. 64:96–102Google Scholar
  16. Mueller, J. F. 1950. Some observations on the structure of hydra, with particular reference to the muscular system. Trans. Am. Microscop. Soc. 69: 133–1347.CrossRefGoogle Scholar
  17. Noda, K. 1968. Migratory cells traversing the mesoglea in hydra. J. Fac, Sci. Hokkaido Univ. Ser. 6 16: 353–358.Google Scholar
  18. Pantin, C. F. A. 1962. Notes on Microscopical Techniques for Zoologists, Cambridge University Press, Cambridge.Google Scholar
  19. Romeis, B. 1968. Mikroskopische Technik, 16th ed., R. Oldenbourg Veriag, Munich. Semai-van Gansen, P. 1954. L’histophysiologie de l’endoderme de l’hydre d’eau douce. Ann. Soc. R. Zool, Belg. 85:217–278Google Scholar
  20. Spangenberg, D. B., and Eakin, R. E. 1962. Histological studies of mechanisms involved in hydra regeneration. J. Exp. Zool. 151: 85–94.CrossRefGoogle Scholar
  21. Znidaric, D. 1970. Comparison of the regeneration of the hypostome with the budding process in Hydra littoralis. Arch. Entwicklungsmech. Org, 166: 45–53.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Richard D. Campbell
    • 1
  1. 1.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

Personalised recommendations