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Affinity Chromatography

  • Chapter
Bioactive Polymeric Systems

Abstract

The separation and purification of groups of biological macromole-cules, such as enzymes, proteins, nucleic acids, and polysaccharides, by conventional procedures often requires considerable experience and expertise since individual members of the groups may differ only slightly in their physicochemical properties. However, one of the most characteristic properties of biological macromolecules is their ability to bind specifically and reversibly to other biomolecules. The technique of affinity chromatography exploits this formation of specific reversible complexes for the resolution of biological macromolecules.(1–3)

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Lowe, C.R., Clonis, Y.D. (1985). Affinity Chromatography. In: Gebelein, C.G., Carraher, C.E. (eds) Bioactive Polymeric Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0405-1_9

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