Abstract
Hypotaurine, 2-aminoethanesulfinic acid, an immediate precursor in taurine biosynthesis (3), is converted to taurine also in brain tissue (10). Hypotaurine concentration in the rat brain is 0.07 mmole/kg fresh weight, i.e. higher than that of the biogenic amine precursors, phenylalanine and tryptophan (21). Taurine transport has been studied in different nervous tissue preparations (16,19) and its penetration into brain tissue has been found to be very slow both in vivo (18) and in vitro (8) when compared to the putative amino acid transmitters. Now we have characterized the properties of hypotaurine by mouse brain slices in vitro. The uptake was found to be strikingly fast and efficient, generating exceptionally high concentration gradients and consisting of two sodium-dependent high-affinity and low-affinity transport systems.
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References
Bond, P.A., 1973, The uptake of gamma-(3H)-aminobutyric acid by slices from various regions of rat brain and the effect of lithium, J. Neurochem., 20: 511–517.
Cavallini, D., De Marco, C. and Mondovi, B., 1958, Sur la synthèse de la thiotaurine, Bull, Soc. Chim. Biol., 40: 1711–1715.
Cavallini, D., Scandurra, R., Duprè, S., Santoro, L., and Barra, D., 1976, A new pathway of taurine biosynthesis, Physiol. Chem. Physics, 8: 157–160.
Davidson, N., Neurotransmitter Amino Acids, Academic Press, London (1976).
Huxtable, R.J., Laird, H.E., II, and Lippincott, S.E., 1979, The transport of taurine in the heart and the rapid depletion of tissue taurine content by guanidinoethyl sulfonate, J. Pharmacol. Exp. Ther., 211: 465–471.
Johnston, G.A.R., Stephanson, A.L., and Twitchin, B., 1976, Uptake and release of nipecotic acid by rat brain slices, J. Neurochem., 26:83–87.
Kontro, P., Comparison of the uptake processes of taurine, hypotaurine and GABA, in: “Amino Acid Transmitters,” P. Mandel and F.V. De Feudis, eds., Raven Press, New York, in press.
Kontro, P., and Oja, S.S., 1978, Taurine uptake by rat brain synaptosomes, J. Neurochem., 30: 1297–1304.
Kontro, P., and Oja, S.S., 1978, Sodium dependence of taurine uptake in rat brain synaptosomes, Neuroscience, 3: 761–765.
Kontro, P., and Oja, S.S., 1980, Hypotaurine oxidation by mouse tissues, in: “Natural Sulfur Compounds: Novel Biochemical and Structural Aspects,” D. Cavallini, V. Zappia and G.E. Gaull, eds., Plenum Press, New York (1980), pp. 201–212.
Kontro, P., and Oja, S.S., Properties of hypotaurine uptake in mouse brain slices, in: “The Action of Taurine on Excitable Cells,” S.I. Baskin, J.J. Kocsis and S.W. Schaffer, eds., Spectrum Publications, New York, in press.
Levi, G., Regional differences in cerebral amino acid transport, in: “Progr. Brain Research, Vol. 29,” A. Lajtha and D.H. Ford, eds., Elsevier, Amsterdam (1968), pp. 219–228.
Martin, D.L., and Shain, W., 1979, High affinity transport of taurine and ß-alanine and low affinity transport of y-aminobutyric acid by a single transport system in cultured glioma cells, J. Biol. Chem., 254: 7076–7084.
Martin, D.L., and Smith, A.A., III, 1972, Ions and the transport of gamma-aminobutyric acid by synaptosomes, J. Neurochem., 19: 841–855.
Oja, S.S., 1971, Exchange of taurine in brain slices of adult and 7-day-old rats, J. Neurochem., 18: 1847–1852.
Oja, S.S., and Kontro, P., Neurotransmitter actions of taurine in the central nervous system, in: “Taurine and Neurological Disorders,” A. Barbeau and R.J. Huxtable, eds., Raven Press, New York (1978), pp. 181–200.
Oja, S.S., and Kontro, P., 1980, Uptake of hypotaurine in brain slices from adult and 8-day-old mice, J. Neurochem., 35: 13031308.
Oja, S.S., Lehtinen, I., and Lähdesmäki, P., 1976, Taurine transport rates between plasma and tissues in adult and 7-dayold mice, Quart, J. exp. Physiol., 61: 133–143.
Oja, S.S., Kontro, P., and Lähdesmäki, P., 1977, Amino acids as inhibitory neurotransmitters, Progr. Pharmacol., 1 /3 1–119.
Okamoto, K., and Namima, M., 1978, Uptake, release and homo-and hetero-exchange diffusions of inhibitory amino acids in guinea-pig cerebellar slices, J. Neurochem., 31: 1393–1402.
Perry, T.L., and Hansen, S., 1973, Quantification of free amino compounds of rat brain: identification of hypotaurine, J. Neurochem., 21: 1009–1011.
Weitsch-Dick, F., Jessel, T.M., and Kelly, J.S., 1978, The selective neuronal uptake and release of (3H)-DL-2,4-diaminobutyric acid by rat cerebral cortex, J. Neurochem., 30: 799–806.
Wheeler, D.D., and Hollingsworth, R.G., 1979, A model of GABA transport by cortical synaptosomes from the Long-Evans rat, J. Neurosci. Res., 4: 265–289.
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© 1982 Plenum Press, New York
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Oja, S.S., Kontro, P. (1982). Hypotaurine Uptake in Mouse Brain Slices. In: Huxtable, R.J., Pasantes-Morales, H. (eds) Taurine in Nutrition and Neurology. Advances in Experimental Medicine and Biology, vol 139. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0402-0_8
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DOI: https://doi.org/10.1007/978-1-4757-0402-0_8
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