Abstract
Ecto-5′-nucleotidase (5′NT) catalyzes the degradation of purine nucleotide-5′-monophosphates to nucleosides and inorganic phosphate; it is an integral plasma membrane enzyme of most mammalian cells and widely used as a membrane marker. Although the physiological role of 5′NT is still mostly obscure, decreased 5′NT activity was found to be associated with a variety of immunological disorders and lymphoproliferative diseases (1–6). 5′NT was suggested to provide a marker for B- and T-lymphocyte maturation (5,7).
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References
Johnson, S. M., G. L. Asherson, R.W.E. Watts, M. E. North, J. Allsop, and A.D.B. Webster. Lymphocyte purine 5′nucleotidase deficiency in primary hypogammaglobulinemia. Lancet I: 168–170, 1977.
Edwards, N. L., D. B. Magilavy, J. T. Cassidy and I. H. Fox. Lymphocyte ecto-5′-nucleotidase deficiency in agammaglobulinemia. Science 201: 628–630, 1978.
Cohen, A., A. Mansour, H.-M. Dosch, and E. W. Gelfand. Association of a lymphocyte purine enzyme deficiency (5′-nucleotidase) with combined immunodeficiency. Clin. Immunol. Immunopathol. 15: 245–250, 1980.
Kramers, M.T.C., D. Catovsky, R. Foa, M. Cherchi and D.A.G. Galton. 5′ nucleotidase activity in leukaemic lymphocytes. Biomedicine 25: 363–365, 1976.
Boss, G. R., L. F. Thompson, H. L. Spiegelberg, T. A. Waldmann, R. D. O’Connor, R. N. Hamburger, and J. E. Seegmiller. Lymphocyte ecto-5′-nucleotidase activity as a marker of B-cell maturation. Trans. Assn. Amer. Phys. 42: 309–315, 1979.
Quagliata, F., D. Faig, M. Conklyn, and R. Silber. Studies on the lymphocyte 5′-nucleotidase in chronic lymphocytic leukemia, infectious mononucleosis, normal subpopulations, and phytohemagglutinin-stimulated cells. Cancer Res. 34: 3197–3202, 1974.
Cohen, A., H. M. Dosch, and E. W. Gelfand Induction of ecto-5′-nucleotidase activity in human thymocytes. Clin. Immunol. Immunopathol. 18: 287–290, 1981.
Pilz, R. B., R. C. Willis and J. E. Seegmiller. Regulation of human lymphoblast plasma membrane 5′ nucleotidase by zinc. J. Biol. Chem. (Submitted for publication).
Levy, J. A., D. N. Buell, C. Creech, Y. Hirshaut, and H. Silverberg. Further characterization of the WIL–1 and WIL–2 lymphoblastoid lines. J. Natl. Can. Inst. 46: 647–652, 1971.
Dvorak, H. F., and L. A. Heppel. Métallo enzymes released from E. coli by osmotic shock. J. Biol. Chem. 243: 2647–2653, 1968.
Drummond, G. I., and Y. Masanobu. Nucleotide phosphomono-esterases. I?: The Enzymes (P.D. Boyer, ed). Vol. 4, 3rd Ed. Academic Press, New York, pp. 337–371, 1971.
Dornand, J., J.-C. Bonnafous, and J.-C. Mani. Purification and properties of 5′-nucleotidase from lymphocyte plasma membranes. Eur. J. Biochem. 87: 459–465, 1978.
Prasad, A. S. Zinc in human nutrition. CRC Press, Boca Raton, Florida, pp. 40–80, 1970.
Frost, P., J. C. Chen, I. Rabbani, J. Smith, and A.S. Prasad. The effect of zinc deficiency on the immune response. In: Zinc Metabolism: Current Aspects in Health and Disease. Alan R. Liss, Inc., New York, pp. 143–150, 1977.
Cunningham-Rundles, S., C. Cunningham-Rundles, B. Dupont, and R. A. Good. Zinc-induced activation of human B lymphocytes. Clin. Immunol. Immunopathol. 16: 115–122, 1980.
Lindeman, R. D., M. L. Clark, and J. P. Calmore. Influence of age and sex on plasma and red-cell zinc concentration. J. Gerontol. 26: 358–363, 1971.
Subcommittee on Zinc (R. I. Henkin, Chairman). National Research Council. Chapt. 9: Clinical Aspects of Zinc Metabolism. University Park Press, Baltimore, pp: 225–248, 1979.
Boss, G. R., L. F. Thompson, H. L. Spiegelberg, W. J. Pichler and J. E. Seegmiller. Age-dependency of lymphocyte ecto-5′-nucleotidase activity. J. Immunol. 125: 679–682, 1980.
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Pilz, R.B., Willis, R.C., Wanlass, S., Seegmiller, J.E. (1984). Regulation of Human Lymphoblast Ecto-5′-Nucleotidase by Zinc. In: De Bruyn, C.H.M.M., Simmonds, H.A., Müller, M.M. (eds) Purine Metabolism in Man-IV. Advances in Experimental Medicine and Biology, vol 165. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0390-0_29
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DOI: https://doi.org/10.1007/978-1-4757-0390-0_29
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