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Nonculturable Microorganisms in the Environment pp 47–55Cite as

Membrane Bioenergetics in Reference to Marine Bacterial Culturability

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Abstract

Understanding of microbial ecology has been expanded by means of isolation, cultivation, and characterization of microorganisms found in natural environments. However, there are still many bacteria which are metabolically active but remain in the so-called “nonculturable” state in nature (44). This state was originally recognized because of the difference observed between direct viable counts and conventional viable counts in seawater (17). Since then, ecological and practical importance of viable but nonculturable cells have been investigated and debated with particular emphasis on Vibrio cholerae and other gram-negative marine bacteria (3, 4, 14, 20, 43, 44). The general features of cells entering the nonculturable state can be summarized as reduction in size (27), decrease in macromolecular synthesis (28), and changes in composition of the cell wall and/or membrane (20, 26). However, little has been investigated with respect to the changes in bioenergetic state.

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Author information

Authors and Affiliations

  1. Ocean Research Institute, University of Tokyo, 1-15-1, Minamidai, Nakano, Tokyo 164, Japan

    Minoru Wada & Kazuhiro Kogure

Authors
  1. Minoru Wada
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  2. Kazuhiro Kogure
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Editors and Affiliations

  1. Center of Marine Biotechnology, Columbus Center, University of Maryland Biotechnology Institute, Baltimore, Maryland, 21202, USA

    Rita R. Colwell

  2. Institute of Marine Sciences, University of Southern Mississippi, Ocean Springs, Mississippi, 39566-7000, USA

    D. Jay Grimes

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© 2000 ASM Press, Washington, D.C.

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Wada, M., Kogure, K. (2000). Membrane Bioenergetics in Reference to Marine Bacterial Culturability. In: Colwell, R.R., Grimes, D.J. (eds) Nonculturable Microorganisms in the Environment. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0271-2_4

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  • DOI: https://doi.org/10.1007/978-1-4757-0271-2_4

  • Publisher Name: Springer, Boston, MA

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  • Online ISBN: 978-1-4757-0271-2

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