Skip to main content

New Approaches to Laboratory Diagnosis

  • Chapter
Coxsackieviruses

Part of the book series: Infectious Agents and Pathogenesis ((IAPA))

Abstract

Classic laboratory techniques to diagnose coxsackievirus (CV)-induced diseases include isolation and typing of the causative agent from body fluids and/or demonstration of virus-specific antibodies in serum or cerebrospinal fluid (CSF) specimens.1,2 These techniques generally are powerful in the diagnosis of viral infections; however, in the case of CV, they are hampered by many difficulties.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Melnick, J. L., Wenner, H. A., and Philips, C. A., 1979, Enteroviruses, in: Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, 5th ed. (E. H. Lennette and N. J. Schmidt, eds.), pp. 471–534, American Public Health Association, Washington, D.C.

    Google Scholar 

  2. Melnick, J. L., 1982, Enteroviruses, in: Viral Infections of Humans, 2nd ed. (A. S. Evans, ed.), pp. 187–251, Plenum, New York.

    Chapter  Google Scholar 

  3. Schmidt, N. J., Ho, H. H., and Lennette, E. H. 1975, Propagation and isolation of group A coxsackieviruses in RD cells, J. Clin. Microbiol. 2:183–185.

    PubMed  CAS  Google Scholar 

  4. Wecker, I., and ter Meulen V., 1977, RD cells in the laboratory diagnosis of enteroviruses, Med. Microbiol. Immunol. 163:233–240.

    Article  PubMed  CAS  Google Scholar 

  5. Landry, M. L., Madore, H. P., Fong, C. K. Y., and Hsiung, G. D., 1981, Use of guinea pig embryo cell cultures for isolation and propagation of group A Coxsackie viruses, J. Clin. Microbiol. 13:588–593.

    PubMed  CAS  Google Scholar 

  6. Schluederberg, A., 1965, Immune globulins in human viral infections, Nature (London) 205:1232–1233.

    Article  Google Scholar 

  7. Schmidt, N.J., Lennette, E. H., and Dennis, J., 1968, Characterization of antibodies produced in natural and experimental coxsackievirus infections, J. Immunol. 100:99–106.

    PubMed  CAS  Google Scholar 

  8. Schmidt, N. J., Magoffin, R. L., and Lennette, E. H., 1973. Association of group B coxsackieviruses with cases of pericarditis, myocarditis, or pleurodynia by demonstration of immunoglobulin M antibody, Infect. Immun. 8:341–348.

    PubMed  CAS  Google Scholar 

  9. Svehag, S. E., and Mandel, B., 1963, The formation and properties of poliovirus neutralizing antibody, J. Exp. Med. 119:1–19.

    Article  Google Scholar 

  10. Mertens, T., Hager, H., and Eggers, H. J., 1982, Epidemiology of an outbreak in a maternity unit of infections with an antigenic variant of echovirus 11, J. Med. Virol. 9:81–91.

    Article  PubMed  CAS  Google Scholar 

  11. Reiner, M., and Wecker, E., 1981, A modified absorption-reduction method to detect virus-specific hemagglutinin inhibiting and neutralizing IgM antibodies, Med. Microbiol. Immunol. 169:237–245.

    Article  PubMed  CAS  Google Scholar 

  12. Richman, D. D., 1983, The use of staphylococcal protein A in diagnostic virology, Curr. Top. Microbiol. Immunol. 104:159–176.

    Article  PubMed  CAS  Google Scholar 

  13. Beck, O. E., 1981, Distribution of virus antibody activity among human IgG subclasses, Clin. Exp. Immunol. 43:626–632.

    PubMed  CAS  Google Scholar 

  14. Fahey, J. L., and Terry, E. W., 1978, Ion exchange chromatography and gel filtration, in: Handbook of Experimental Immunology, 3rd ed. (D. M. Weir, ed.), pp. 8.1–8.16, Blackwell Scientific Publications, Oxford.

    Google Scholar 

  15. Johnson, R. B., and Libby, R., 1980, Separation of immunoglobulin M (IgM) essentially free of IgG from serum for use in systems requiring assay of IgM-type antibodies without interference from rheumatoid factor. J. Clin. Microbiol. 12:451–454.

    PubMed  CAS  Google Scholar 

  16. Sann, G., Schneider, G., Loeke, S., and Doerr, H. W., 1983, Rapid fractionation of serum immunoglobulins by high pressure liquid gel permeation chromatography. Applications to routine serologic procedures, J. Immunol. Methods 59:121–127.

    Article  PubMed  CAS  Google Scholar 

  17. Sampson, I. A., Hodgen, A. N., and Arthur, I. H., 1984, The separation of IgM from human serum by FPLC, J. Immunol. Methods 69:9–15.

    Article  PubMed  CAS  Google Scholar 

  18. Yolken, R. H., 1982, Enzyme immunoassay for the detection of infectious antigens in body fluids, Rev. Infect. Dis. 4:35–68.

    Article  PubMed  CAS  Google Scholar 

  19. Yolken, R. H., and Torsch, V., 1980, Enzyme-linked immunosorbent assay for the detection and identification of Coxsackie B antigen in tissue cultures and clinical specimens, J. Med. Virol. 6:45–52.

    Article  PubMed  CAS  Google Scholar 

  20. Yolken, R. H., and Torsch, V. M., 1981, Enzyme-linked immunosorbent assay for detection and identification of coxsackieviruses A, Infect. Immun. 31:742–750.

    PubMed  CAS  Google Scholar 

  21. Kulski, J. K., and Norval, M., 1985, Nucleic acid probes in diagnosis of viral diseases of man, Arch, Virol. 83:3–15.

    Article  CAS  Google Scholar 

  22. Hyypiä, T., Stålhandske, P., Vainionpää, R., and Pettersson, U., 1984, Detection of enteroviruses by spot hybridization, J. Clin. Microbiol. 19:436–438.

    PubMed  Google Scholar 

  23. Bowles, N. E., Richardson, P. J., Olsen, E. G. J., and Archard, L. C., 1986, Detection of coxsackie-B-virus-specific RNA sequences in myocardial biopsy samples from patients with myocarditis and dilated cardiomyopathy, Lancet 1:1120–1123.

    Article  PubMed  CAS  Google Scholar 

  24. Tracy, S., 1984, A comparison of genomic homologies among the coxsackievirus B group: Use of fragments of the cloned coxsackievirus B3 genome as probes, J. Gen. Virol 65:2167–2172.

    Article  PubMed  CAS  Google Scholar 

  25. Kandolf, R., Ameis, D., Kirschner, P., Canu, A., and Hofschneider, P. H., 1986, In situ detection of viral genomes in myocardial cells by nucleic acid hybridization: A novel approach to the diagnosis of viral heart disease, presented at the International Symposium on Inflammatory Heart Disease, May 15–17, Würzburg, Federal Republic of Germany.

    Google Scholar 

  26. Kandolf, R., and Hofschneider, P. H., 1985, Molecular cloning of the genome of a cardiotropic Coxsackie B3 virus: Full-length reverse-transcribed recombinant c-DNA generates infectious virus in mammalian cells, Proc. Natl. Acad. Sci. USA 82:4818–4822.

    Article  PubMed  CAS  Google Scholar 

  27. Prabhakar, B. S., Haspel, M. V., McClintock, P. R., and Notkins, A. L., 1982, High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies, Nature (Lond.) 300:374–376.

    Article  CAS  Google Scholar 

  28. Prabhakar, B. S., Menegus, M. A., and Notkins, A. L., 1985. Detection of conserved and nonconserved epitopes on coxsackievirus B4: Frequency of antigenic change, Virology 146:302–306.

    Article  PubMed  CAS  Google Scholar 

  29. Cao, Y., Schnurr, D. P., and Schmidt, N. J., 1984, Monoclonal antibodies for study of antigenic variation in coxsackievirus type B4: Association of antigenic determinants with myocarditic properties of the virus, J. Gen. Virol. 65:925–935.

    Article  PubMed  Google Scholar 

  30. Pattison, J. R., 1983, Tests for Coxsackie B virus-specific IgM, J. Hyg. (Lond.) 90:327–332.

    Article  CAS  Google Scholar 

  31. Dörries, R., and ter Meulen, V., 1980, Detection of enterovirus specific IgG and IgM antibodies in humans by an indirect solid phase radioimmunoassay, Med. Microbiol. Immunol. 168:159–171.

    Article  PubMed  Google Scholar 

  32. Dörries, R., and ter Meulen V., 1983, Specificity of IgM antibodies in acute human coxsackievirus B infections, analysed by indirect solid phase enzyme immunoassay and immunoblot technique, J. Gen. Virol. 64:159–167.

    Article  PubMed  Google Scholar 

  33. El-Hagrassy, M. M. O., and Banatvala, J. E., 1980, Coxsackie-B-virus-specific IgM responses in patients with cardiac and other diseases, Lancet 2:1160–1162.

    Article  PubMed  CAS  Google Scholar 

  34. Chan, D., and Hammond, G. W., 1985, Comparison of serodiagnosis of group B coxsackievirus infections by an immunoglobulin M capture enzyme immunoassay versus microneutralization, J. Clin. Microbiol. 21:830–834.

    PubMed  CAS  Google Scholar 

  35. McCartney, R. A., Banatvala, J. E., and Bell, E. J., 1986, Routine use of μ-antibody-capture ELISA for the serological diagnosis of Coxsackie B virus infections, J. Med. Virol. 19:205–212.

    Article  PubMed  CAS  Google Scholar 

  36. Bell, E. J., McCarney, R. A., Basquill, D., and Chaudhuri, A. K. R., 1986, μ-Antibody capture ELISA for the rapid diagnosis of enterovirus infections in patients with aseptic meningitis, J. Med. Virol. 19:213–217.

    Article  PubMed  CAS  Google Scholar 

  37. Meurman, O., 1983, Detection of antiviral IgM antibodies and its problems—A review, Curr. Top. Microbiol. Immunol. 104:101–131.

    Article  PubMed  CAS  Google Scholar 

  38. Ziegler, D. W., 1979, Determination of IgM antibodies in diagnostic virology, in: Diagnosis of Viral Infecfions (D. A. Lenette, S. Pecter, and K. D. Thompson, eds.), pp. 63–71, University Park Press, Baltimore.

    Google Scholar 

  39. Salonen, E.-M., Vaheri, A., Suni, J., and Wager, O., 1980, Rheumatoid factor in acute viral infections: Interference with determination of IgM, IgG and IgA antibodies in an enzyme immunoassay, J. Infect. Dis. 142:250–255.

    Article  PubMed  CAS  Google Scholar 

  40. Hannington, G., Booth, J. C., Wiblin, C. N., and Stern, H., 1983, Indirect enzyme-linked immunosorbent assay (ELISA) for detection of IgG antibodies against Coxsackie B viruses, J. Med. Microbiol. 16:459–465.

    Article  PubMed  CAS  Google Scholar 

  41. Pugh, S. F., 1984, Heterotypic reactions in a radioimmunoassay for Coxsackie B virus specific IgM, J. Clin. Pathol. 37:433–439.

    Article  PubMed  CAS  Google Scholar 

  42. Morgan-Capner, P., and McSorley, C., 1983, Antibody capture radioimmunoassay (MACRIA) for coxsackievirus B4 and B5-specific IgM, J. Hyg. (Lond.) 90:333–349.

    Article  CAS  Google Scholar 

  43. Banatvala, J. E., Bryant, J., Schernthaner, G., Borkenstein, M., Schober, E., Brown, D., De Silva, L. M., Menser, M. A., and Silink, M., 1985, Coxsackie B, mumps, rubella and cytomegalovirus specific IgM responses in patients with juvenile-onset insulin-dependent diabetes mellitus in Britain, Austria and Australia, Lancet 1:1409–1412.

    Article  PubMed  CAS  Google Scholar 

  44. Mertens, Th., Pika, U., and Eggers, H. J., 1983, Cross antigenicity among enteroviruses as revealed by immunoblot technique, Virology 129:431–442.

    Article  PubMed  CAS  Google Scholar 

  45. Katze, M. G., and Crowell, R. L., 1980, Immunological studies of the group B coxsackieviruses by the sandwich enzyme-linked immunosorbent assay (ELISA) and im-munoprecipitation, J. Gen. Virol. 50:357–367.

    Article  PubMed  CAS  Google Scholar 

  46. Eggers, H. J., and Mertens, T., 1986, Persistence of Coxsackie B virus-specific IgM, Lancet 2:284.

    Article  PubMed  CAS  Google Scholar 

  47. Tilzey, A. T., Signy, M., and Banatvala, J. E., 1986, Persistent Coxsackie B virus specific IgM response in patients with recurrent pericarditis, Lancet 1:1491–1492.

    Article  PubMed  CAS  Google Scholar 

  48. Halonen, P., Meurman, O., Lövgren, T., Hemmilä, I., and Soini, E., 1983, Detection of viral antigens by time-resolved fluoroimmunoassay Curr. Top. Microbiol. Immunol. 104:133–146.

    Article  PubMed  CAS  Google Scholar 

  49. Boguslawski, S. J., Smith, D. E., Michalak, M. A., Mickelson, K. E., Yehle, C. O., Patterson, W. L., and Carrico, R. J., 1986, Characterization of monoclonal antibody to DNA-RNA and its application to immunodetection of hybrids, J. Immunol. Methods 89:123–130.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1988 Plenum Press, New York

About this chapter

Cite this chapter

Dörries, R. (1988). New Approaches to Laboratory Diagnosis. In: Bendinelli, M., Friedman, H. (eds) Coxsackieviruses. Infectious Agents and Pathogenesis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0247-7_12

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-0247-7_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0249-1

  • Online ISBN: 978-1-4757-0247-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics