Skip to main content

Anther-Specific Gene Expression in Brassica and Arabidopsis

  • Chapter
  • 101 Accesses

Abstract

Pollen, the male gametophyte of flowering plants, plays a vital role in sexual reproduction and hence seed set. Pollen grains are the haploid products of the meiotic divisions of the diploid microsporocytes, which reside in the anther (Fig. 2–1). This developmental process requires an intimate interaction between two generations: the diploid sporophyte including the microsporocytes and anther tissues and the gametophyte (Knox, 1984). More than 26 developmental characters of male reproductive development have now been described, although for only a limited range of taxa (Blackmore et al., 1987). The character states for Brassica campestris pollen development are presented in Figure 2–2, and Table 2–2 which is based on data collected by Knox (1987).

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Albani, D., L. S. Robert, P. E. Donaldson, I. Altosaar, P. G. Arnison, and S. F. Fabijanski, 1990. Characterization of a pollen-specific gene family from Brassica napus which is activated during early microspore development. Plant Mol. Biol. 15:605–622.

    Article  PubMed  CAS  Google Scholar 

  • Albani, D., I. Altosaar, P. G. Arnison, and S. F. Fabijanski. 1991. A gene showing sequence similarity to pectin esterase is specifically expressed in developing pollen of Brassica napus. Sequences in its 5′ flanking region are conserved in other pollen-specific promoters. Plant Mol. Biol. 16:501–513.

    Article  PubMed  CAS  Google Scholar 

  • Albani, D., R. Sardana, L. S. Robert, I. Altosaar, P. G. Arnison, and S. F. Fabijanski. 1992. A Brassica napus gene family which shows sequence similarity to ascorbate oxidase is expressed in developing pollen. Molecular characterization and analysis of promoter activity in transgenic tobacco plants. Plant J. 2(3):331–342.

    PubMed  CAS  Google Scholar 

  • Blackmore, S., C. McConchie, and R. B. Knox. 1987. Phylogenetic analysis of the male ontogenetic program in aquatic and terrestrial monocotyledons. Cladistics 3:333–347.

    Article  Google Scholar 

  • Brown, S. M. and Crouch, M. L. 1990. Characterization of a gene family abundantly expressed in Oenothera organensis pollen that shows sequence similarity to polygalacturonase. Plant Cell 2:263–274.

    Article  PubMed  CAS  Google Scholar 

  • Davies, S. P., M. B. Singh, and R. B. Knox. 1992. Identification and in situ localization of pollen-specific genes. Int. Rev. Cytol. 140:19–34.

    Article  CAS  Google Scholar 

  • Foster, G. D., R. P. Robinson, M. R. Blundell, R. Hodge, J. Draper, and R. J. Scott. 1992. A Brassica napus mRNA encoding a protein homologous to phospholipid transfer proteins is expressed specifically in the tapetum and developing microspores. Plant Sci. 84:187–192.

    Article  CAS  Google Scholar 

  • Hamilton, D. A., D. M. Bashe, J. R. Stinson, and J. P. Mascarenhas. 1989. Characterization of a pollen-specific genomic clone from maize. Sex Plant Reprod. 2:208–212.

    Article  Google Scholar 

  • Hanson, D. D., D. A. Hamilton, J. L. Travis, D. M. Bashe, and J. P. Mascarenhas. 1989. Characterization of a pollen-specific cDNA clone from Zea mays and its expression. Plant Cell 1:173–179.

    Article  PubMed  CAS  Google Scholar 

  • Hodge, R., W. Paul, J. Draper, and R. Scott. 1992. Cold-plaque screening: a simple technique for the isolation of low abundance, differentially expressed transcripts from conventional cDNA libraries. Plant J. 2(2):257–260.

    Article  CAS  Google Scholar 

  • Jefferson, R. A., S. M. Burgess, and D. Hirsh. 1986. ß-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc. Natl. Acad. Sci. USA 83:8447–8451.

    Article  PubMed  CAS  Google Scholar 

  • Jefferson, R. A., T. A. Kavanagh, and M. W. Bevan. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6:3901–3907.

    PubMed  CAS  Google Scholar 

  • Koltunow, A. M., J. Truettner, K. H. Cox, M. Wallroth, and R. B. Goldberg. 1990. Different temporal and spatial gene expression patterns occur during anther development. Plant Cell 2:1201–1224.

    Article  PubMed  CAS  Google Scholar 

  • Knox, R. B. 1984. The pollen grain. In Embryology of Angiosperms, ed. B. M. Johri, pp. 197–272. Springer-Verlag, Berlin.

    Chapter  Google Scholar 

  • Knox, R. B. 1987. Pollen differentiation patterns and male function. In Differentiation Patterns in Higher Plants, ed. K. M. Urbanska, pp. 33–52, Academic Press, London.

    Google Scholar 

  • Knox, R. B. and J. Heslop-Harrison. 1969. Cytochemical localization of enzymes in the wall of the pollen grains. Nature 223:92–94.

    Article  CAS  Google Scholar 

  • Mariani, C., M. De Blockeleer, J. Truettner, J. Leemans, and R. B. Goldberg, 1990. Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 347:737–741.

    Article  CAS  Google Scholar 

  • Mascarenhas, J. P. 1992. Pollen gene expression: Molecular evidence. Int. Rev. Cytol. 140:3–18.

    Article  PubMed  CAS  Google Scholar 

  • Pacini, E. 1990. Tapetum and microspore function. In Microspores: Evolution and Ontogeny, eds. S. Blackmore and R. B. Knox, pp. 213–237, Academic Press, London.

    Google Scholar 

  • Paul, W., Hodge, R., Smartt, S., Draper, J. and Scott, R. 1992. The isolation and characterization of the tapetum-specific Arabidopsis thaliana A9 gene. Plant Mol. Biol. 19:611–622.

    Article  PubMed  CAS  Google Scholar 

  • Roberts, M. R., Foster, G. D., Blundell, R. P., Robinson, S. W., Kumar, A., Draper, J. and Scott, R. 1993. Gametophytic and sporophytic expression of an anther specific Arabidopsis thaliana gene. The Plant J. 3:111–120.

    Article  CAS  Google Scholar 

  • Smith, C.J.S., C. F. Watson, J. Ray, C. R. Bird, and P. C. Morris. 1988. Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334:724–726.

    Article  CAS  Google Scholar 

  • Smith, C.J.S., C. F. Watson, J. Ray, C. R. Bird, and P. C. Morris. 1990. Inheritance and effect on ripening of antisense polygalacturonase gene in transgenic tomatoes. Plant Mol. Biol. 14:369–379.

    Article  PubMed  CAS  Google Scholar 

  • Stieglitz, G. and H. Stern. 1973. Regulation of ß-l,3-glucanase activity of developing anthers of lilium. Develop. Biol. 34:169–173.

    Article  PubMed  CAS  Google Scholar 

  • Stinson, J. R., A. J. Eisenberg, R. F. Willing, P. M. Enrico, D. D. Hanson, and J. P. Mascarenhas. 1987. Genes expressed in the male gametophyte of flowering plants and their isolation. Plant Physiol. 83:442–447.

    Article  PubMed  CAS  Google Scholar 

  • Theerakulpisut, P., H. L. Xu, M. B. Singh, J. M. Pettitt, and R. B. Knox. 1991. Isolation and developmental expression of Bcpl, an anther-speciifc cDNA clone in Brassica campestris. Plant Cell. 3:1073–1084.

    CAS  Google Scholar 

  • Twell, D., R. A. Wing, J. Yamaguchi, and S. McCormick. 1989. Isolation and expression of an anther-specific gene from tomato. Mol. Gen. Genet. 217:240–245.

    Article  PubMed  CAS  Google Scholar 

  • Twell, D., J. Yamaguchi, R. A. Wing, J. Ushiba, and S. McCormick. 1991. Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements. Genes Dev. 5:496–507.

    Article  PubMed  CAS  Google Scholar 

  • van der Krol, A. R., P. E. Lenting, J. Veenstra, I. M. van der Meer, R. E. Koes, A.G.M. Gerätes, and A. R. Stuitje. 1988. An antisense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature 333:866–869.

    Article  Google Scholar 

  • van der Meer, I. M., M. E. Stam, A. J. van Tunen, J.N.M. Mol, and A. R. Stuitje. 1992. Antisense inhibition of flavonoid biosynthesis in petunia anthers results in male sterility. Plant Cell 4:253–262.

    Article  PubMed  Google Scholar 

  • Visser, R.G.F., I. Somhorst, G. J. Kuipers, N. J. Ruys, W. J. Feenstra, and E. Jacobsen. 1991. Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs. Mol. Gen. Genet. 225:289–296.

    Article  PubMed  CAS  Google Scholar 

  • Willing, R. P. and J. P. Mascarenhas. 1984. Analysis of the complexity and diversity of mRNAs from pollen and shoots of Tradescantia. Plant Physiol. 75:865–868.

    Article  PubMed  CAS  Google Scholar 

  • Willing, R. P., D. Bashe, and J. P. Mascarenhas. 1988. An analysis of the quantity and diversity of messenger RNAs from pollen and shoots of Zea mays. Theor. Appl. Genet. 75:751–753.

    Article  CAS  Google Scholar 

  • Wing, R. A., J. Yamaguchi, S. K. Larabell, V. M. Ursin, and S. McCormick. 1989. Molecular and genetic characterization of two pollen expressed genes that have sequence similarity to pectate lyases of the plant pathogen Erwinia. Plant Mol. Biol. 14:17–28.

    Article  Google Scholar 

  • Worrall, D., D. L. Hird, R. Hodge, W. Paul, J. Draper, and R. Scott. 1992. Premature dissolution of the microsporocyte callose wall causes male sterility in transgenic tobacco. Plant Cell 4:759–771.

    Article  PubMed  CAS  Google Scholar 

  • Xu, H. 1992. Molecular analysis of anther-specific genes from Brassica campestris and Arabidopsis thaliana. Ph.D. Thesis, University of Melbourne.

    Google Scholar 

  • Xu, H., S. P. Davies, B.Y.H. Kwan, A. P. O’Brien, M. B. Singh, and R. B. Knox. 1993. Haploid and diploid expression of a Brassica campestris anther-specific gene promoter in Arabidopsis and tobacco. Mol. Gen. Genet. 239:58–65.

    PubMed  CAS  Google Scholar 

  • Xu, H., Knox, R. B., Taylor, P. E. and Singh, M. B. 1995. Bcpl, a gene required for male fertility in Arabidopsis. Proc. Natl. Acad. Sci. USA 92(6):2106–2110.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Chapman & Hall

About this chapter

Cite this chapter

Xu, H., Knox, R.B., Singh, M.B. (1996). Anther-Specific Gene Expression in Brassica and Arabidopsis . In: Mohapatra, S.S., Knox, R.B. (eds) Pollen Biotechnology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0235-4_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-0235-4_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0237-8

  • Online ISBN: 978-1-4757-0235-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics