Analytical and Preparative Polyacrylamide Gel Electrophoresis

Chrambach, A.; Rodbard, D.; Jovin, T. M.; Svendsen, P. J.

doi:10.1007/978-1-4684-9984-1_2

A. Chrambach³,
D. Rodbard³,
T. M. Jovin⁴ &
…
P. J. Svendsen⁵

Part of the book series: Biological Separations ((BIOSEP))

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66 Citations

Abstract

Biochemistry may be viewed as the attempt to fragment living matter to its component molecular species, and to elucidate the chemical reactions of these species first in two-component systems, then in ever more complex systems and finally to reconstitute life as the sum of its component chemical reactions. The first task, i.e., the isolation of nature’s building-block molecules, remains a formidable task. This chapter is concerned with the instrumentation and procedures available for isolation of any given charged species according to a generally applicable fractionation strategy.

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References

Axelsen, N. H., Kroll, J., and Weeke, B., eds. (1973). A Manual of Quantitative Immunelectrophoresis, Universitetsforlaget, Oslo, Bergen, Tromsø.
Google Scholar
Ballou, B., Sundharadas, G., and Bach, M. L. (1974). Science 185:531.
Article PubMed CAS Google Scholar
Baumann, G., and Chrambach, A. (1975a). Anal. Biochem. 64:530.
Article PubMed CAS Google Scholar
Baumann, G., and Chrambach, A. (1975b). In Progress in Isoelectric Focusing and Isotachophoresis (P. G. Righetti, ed.), Associated Scientific Publishers, Amsterdam, p. 13.
Google Scholar
Baumann, G., and Chrambach, A. (1976a). Proc. Natl. Acad. Sci., USA 73:732.
Article PubMed CAS Google Scholar
Baumann, G., and Chrambach, A. (1976b). Anal. Biochem. 70:32.
Article PubMed CAS Google Scholar
Ben-David, M., and Chrambach, A. (1973). Acta Endocrinol. 72:654.
PubMed CAS Google Scholar
Ben-David, M., Becker, R., Rodbard, D., and Chrambach, A. (1974). Endocrinol. Res. Commun. 1:211.
Article Google Scholar
Bergram, B., and Harlestam, R. (1968). Sci. Tools 15:26.
Google Scholar
Bøg-Hansen, T. C. (1973). Anal. Biochem. 56:480.
Article PubMed Google Scholar
Bøg-Hansen, T. C., Svendsen, P. J., Berrum, O. J., Nielsen, C. S., and Remlau, J. (1974). In Protides of the Biological Fluids, Vol. 22 (H. Peeters, ed.), Pergamon Press, Oxford.
Google Scholar
Bøg-Hansen, T. C., Svendsen, P. J., and Bjerrrum, O. J. (1975). In Progress in Isoelectric Focusing and Isotachophoresis (P. G. Righetti, ed.), Associated Scientific Publishers, Amsterdam, p. 347.
Google Scholar
Bonner, W. M., and Laskey, R. A. (1974). Eur. J. Biochem. 46:83.
Article PubMed CAS Google Scholar
Brisgunov, V., Rebentish, B., and Debanov, V. (1975). Anal. Biochem. 66:100.
Article PubMed CAS Google Scholar
Brogren, C.-H., and Svendsen, P. J. (1974). In Protides of the Biological Fluids, Vol. 22 (H. Peeters, ed.), Pergamon Press, Oxford, p. 685.
Google Scholar
Brogren, C.-H., Svendsen, P. J., and Bøg-Hansen, T. C. (1975). In Progress in Isoelectric Focusing and Isotachophoresis (P. G. Righetti, ed.), Associated Scientific Publishers, Amsterdam, p. 359.
Google Scholar
Brown, W. D., and Green, S. (1970). Anal. Biochem. 34:595.
Article Google Scholar
Brownstone, D. (1969). Anal. Biochem. 27:25.
Article PubMed CAS Google Scholar
Cantz, M., Chrambach, A., Bach, G., and Neufeld, E. (1972). J. Biol. Chem. 247:5456.
PubMed CAS Google Scholar
Carpenter, F. H., and Harrington, K. T. (1972). J. Biol. Chem. 247:5580.
PubMed CAS Google Scholar
Catsimpoolas, N. (1974a). Sep. Sci. 10:55.
Google Scholar
Catsimpoolas, N. (1974b). Sep. Sci. 9:491ff.
Article Google Scholar
Catsimpoolas, N., Griffith, A. L., Williams, J. M., Chrambach, A., and Rodbard, D. (1975). Anal. Biochem. 69:372.
Article PubMed CAS Google Scholar
Chen, C. S. H. (1965). J. Polymer Sci. Part A 3:1107.
Google Scholar
Chidakel, B. E., Baumann, G., Rodbard, D., and Chrambach, A. (1975). Anal. Biochem. 66:540.
Article PubMed CAS Google Scholar
Chrambach, A., and Baumann, G. (1976). In Isoelectric Focusing (N. Catsimpoolas, ed.), in press, Academic Press, New York.
Google Scholar
Chrambach, A., and Rodbard, D. (1971). Science 172: 440.
Article PubMed CAS Google Scholar
Chrambach, A., and Rodbard, D. (1972). Sep. Sci. 7:663.
Article CAS Google Scholar
Chrambach, A., and Skyler, J. D. (1974). In Protides of the Biological Fluids, Vol. 22 (H. Peeters, ed.), Pergamon Press, Oxford, p. 701.
Google Scholar
Chrambach, A., Reisfeld, R. A., Wyckoff, M., and Zaccari, J. (1967). Anal. Biochem. 20: 150.
Article PubMed CAS Google Scholar
Chrambach, A., Cantz, M., and Kapadia, G. (1972a). Sep. Sci. 7:747.
Article Google Scholar
Chrambach, A., Hearing, E., Lunney, J., and Rodbard, D. (1972b). Sep. Sci. 7:725.
Article CAS Google Scholar
Chrambach, A., Pickett, J., Schlam, M. L., Kapadia, G., and Holtzman, N. A. (1972c). Sep. Sci. 7:773.
Article CAS Google Scholar
Chrambach, A., Doerr, P., Finlayson, G. R., Miles, L. E. M., Sherins, R., and Rodbard, D. (1973). Ann. N.Y. Acad. Sci. 209:44.
Article PubMed CAS Google Scholar
Clemmensen, I. (1973). Sci. Tools 20:7.
CAS Google Scholar
Clemmensen, I., and Svendsen, P. J. (1973). Sci. Tools 20:5.
CAS Google Scholar
Cole, E. G., and Mecham, D. K. (1966). Anal. Biochem. 14:215.
Article PubMed CAS Google Scholar
Davis, B. J. (1964). Ann. N.Y. Acad. Sci. 121:404.
Article PubMed CAS Google Scholar
Datyner, A., and Finimore, E. (1973). Anal. Biochem. 52:45.
Article PubMed CAS Google Scholar
Dietrich, C. P. (1973). Anal. Biochem. 51:345.
Article PubMed CAS Google Scholar
Diezel, W., Kopperschlaeger, G., and Hoffman, E. (1972). Anal. Biochem. 48: 617.
Article PubMed CAS Google Scholar
Dirksen, M. L., and Chrambach, A. (1972). Sep. Sci. 7:747.
Article CAS Google Scholar
Fairbanks, G., Levinthal, C., and Reeder, R. H. (1965). Biochem. Biophys. Res. Commun. 20:393.
Article PubMed CAS Google Scholar
Fairbanks, G., Steck, T. L., and Wallach, D. F. H. (1971). Biochemistry 10:2606.
Article PubMed CAS Google Scholar
Fawcett, J. S. (1973). Ann. N.Y. Acad. Sci. 209:112.
Article PubMed CAS Google Scholar
Felgenhauer, K. (1974). Z. Physiol. Chem. 355:1281.
Article CAS Google Scholar
Ferguson, K. A. (1964). Metabolism 13:985.
Article PubMed CAS Google Scholar
Finlayson, R., and Chrambach, A. (1971). Anal. Biochem. 40:292.
Article PubMed CAS Google Scholar
Frank, R. N., and Rodbard, D. (1975). Arch. Biochem. Biophys. 171:1.
Article PubMed CAS Google Scholar
Griffith, I. P. (1972). Anal. Biochem. 46:402.
Article PubMed CAS Google Scholar
Hearing, V. J., Klingler, W. G., Ekel, T. M., and Montague, P. M. (1976). Anal. Biochem. 72:113.
Article PubMed CAS Google Scholar
Hedrick, J. L., and Smith, A. J. (1968). Arch. Biochem. Biophys. 126:154.
Article Google Scholar
Hjerten, S., Jerstedt, S., and Tiselius, A. (1969). Anal. Biochem. 27:108.
Article PubMed CAS Google Scholar
Hoffman, H., Naughton, M. A., McDougall, J., and Hamilton, E. A. (1967). Nature 214:703.
Article PubMed CAS Google Scholar
Johansson, B. G. (1972). Scand. J. Clin. Lab. Invest. 29:7 (suppl. 124).
Article CAS Google Scholar
Jovin, T. M. (1973a). Biochemistry 12:871, 879, 890.
Article Google Scholar
Jovin, T. M. (1973b). Ann. N.Y. Acad. Sci. 209:477.
Article PubMed CAS Google Scholar
Jovin, T., Chrambach, A., and Naughton, M. A. (1964). Anal. Biochem. 9:351.
Article PubMed CAS Google Scholar
Jovin, T. M., Dante, M. L., and Chrambach, A. (1970). Multiphasic Buffer Systems Output, National Technical Information Service, Springfield, Va. 22151, PB No. 196085–196091, 203016.
Google Scholar
Just, W. W., Leon V. J. O., and Werner, G. (1975). Anal. Biochem. 67:590.
Article PubMed CAS Google Scholar
Kapadia, G., and Chrambach, A. (1972). Anal. Biochem. 48:90. (Appendix II: Preparative PAGE procedure: Apparatus B. Appendix III : preparative PAGE procedure: Apparatus C and D.)
Article PubMed CAS Google Scholar
Kapadia, G., Chrambach, A., and Rodbard, D. (1974). Electrophoresis and Isoelectric Focusing on Polyacrylamide Gel (R. C. Allen and H. R. Maurer, eds.), Walter de Gruyter, Berlin, p. 115.
Google Scholar
Klotz, I. M., and Darwall, D. W. (1973). In Handbook of Biochemistry, 2nd ed. (H. A. Sober, ed.), CRC Press, Cleveland, p. 62.
Google Scholar
Kohler, P. O., Bridson, W. E., and Chrambach, A. (1971). J. Clin. Endocrinol. 32:70.
Article CAS Google Scholar
Loening, U. E. (1967). Biochem. J. 102:251.
PubMed CAS Google Scholar
McCormick, A., Miles, L. E. M., and Chrambach, A. (1976). Anal. Biochem. 75:314.
Article PubMed CAS Google Scholar
McIlwaine, I., Rodbard, D., and Chrambach, A. (1973). Anal. Biochem. 55:421.
Article Google Scholar
Morris, C. J. O. R., and Morris, P. (1971). Biochem. J. 124:517.
PubMed CAS Google Scholar
Neville, D. M. (1968). Biochim. Biophys. Acta 154:540.
Article PubMed CAS Google Scholar
Nguyen, N. Y., and Chrambach, A., (1976a). Anal. Biochem. 74:145.
Article PubMed CAS Google Scholar
Nguyen, N. Y., Rodbard, D., Svendsen, P. J., and Chrambach, A. (1976b). Anal. Biochem. (in press).
Google Scholar
Nishihara, M., Chrambach, A., and Aposhian, H. V. (1967). Biochemistry 6:1877.
Article PubMed CAS Google Scholar
O’Farrell, P. H. (1975). J. Biol. Chem. 250:4007.
PubMed Google Scholar
Ornstein, L. L. (1964). Ann. N.Y. Acad. Sci. 121:321.
Article PubMed CAS Google Scholar
Oster, G. K., Oster, G., and Prati, G. (1957). J. Am. Chem. Soc. 79:595.
Article CAS Google Scholar
Peterson, J. I., Tipton, H. W., and Chrambach, A. (1974). Anal. Biochem. 62:274.
Article PubMed CAS Google Scholar
Radola, B. J. (1973). Biochim. Biophys. Acta 295:412.
Article PubMed CAS Google Scholar
Radola, B. J. Ann. N.Y. Acad. Sci. 209:127.
Google Scholar
Reisfeld, R. A., Lewis, U. J., and Williams, D. E. (1962). Nature 195:281.
Article PubMed CAS Google Scholar
Reisfeld, R. A., Borjeson, J., Chessin, L. N., and Small, P. A., Jr. (1967). Proc. Natl. Acad. Sci. USA 58:2020.
Article PubMed CAS Google Scholar
Reisner, A. H., Nemes, P., and Bucholtz, C. (1975). Anal. Biochem. 64:509.
Article PubMed CAS Google Scholar
Reynolds, J., and Tanford, C. (1970). J. Biol. Chem. 245:5161.
PubMed CAS Google Scholar
Rodbard, D., and Chrambach, A. (1970). Proc. Natl. Acad. Sci. USA 65:970.
Article PubMed CAS Google Scholar
Rodbard, D., and Chrambach, A. (1971). Anal. Biochem. 40:95.
Article PubMed CAS Google Scholar
Rodbard, D., and Chrambach, A. (1974). In Electrophoresis and Isoelectric Focusing on Polyacrylamide Gel (R. C. Allen and H. R. Maurer, eds.), Walter de Gruyter, Berlin, p. 28.
Google Scholar
Rodbard, D., Kapadia, G., and Chrambach, A. (1971). Anal. Biochem. 40:135.
Article PubMed CAS Google Scholar
Rodbard, D., Levitov, C., and Chrambach, A. (1972). Sep. Sci. 7:705.
Article CAS Google Scholar
Rodbard, D., Chrambach, A., and Weiss, G. (1974). In Electrophoresis and Isoelectric Focusing on Polyacrylamide Gel (R. C. Allen and H. R. Maurer, eds.), Walter de Gruyter, Berlin, p. 62.
Google Scholar
Routs, R. J. (1971). Electrolyte systems in isotrachophoresis and their application to some protein separations. Thesis, Technische Hogeschool te Eindhoven, the Netherlands, Solna Skrivand Stenograftjaenst AB, Solna, Sweden.
Google Scholar
Routs, R. J. (1973). Ann. N.Y. Acad. Sci. 209:445.
Article PubMed CAS Google Scholar
Stenman, U.-H. (1975). In Progress in Isoelectric Focusing and Isotachophoresis (P. G. Righetti, ed.), Associated Scientific Publishers, Amsterdam, p. 39.
Google Scholar
Strauch, L. (1967). In Protides of the Biological Fluids, Vol. 15 (H. Peeters, ed.), Pergamon Press, Oxford, p. 535.
Google Scholar
Svendsen, P. J. (1972). Sci Tools 19:21.
CAS Google Scholar
Svendsen, P. J. (1973). Sci Tools 20:1.
CAS Google Scholar
Svendsen, P. J., and Rose, C. (1970). Sei. Tools 17:13.
CAS Google Scholar
Tanford, C. (1961). Physical Chemistry of Macromolecules, Wiley, New York.
Google Scholar
Tipton, H. W., Rumen, N. M., and Chrambach, A. (1975). Anal. Biochem. 69:323.
Article PubMed CAS Google Scholar
Ugel, A. R., Chrambach, A., and Rodbard, D. (1971). Anal. Biochem. 43:410.
Article PubMed CAS Google Scholar
Uriel, J., and Berges, J. (1966). C. R. Acad. Sei. 262:164.
CAS Google Scholar
Uriel, J., and Berges, J. (1974). In Electrophoresis and Isoelectric Focusing on Polyacrylamide Gel (R. C. Allen and H. R. Maurer, eds.), Walter de Gruyter, Berlin, p. 235.
Google Scholar
Vaitukaitis, J., Robbins, J. B., Nieschlag, E., and Ross, G. T. (1971). J. Clin. Endocrinol. Metab. 33:988.
Article PubMed CAS Google Scholar
Vesterberg, O. (1973). ScL Tools 20:22.
CAS Google Scholar
Vogel, O., and Reinmuth, H. (1975). Anal. Biochem. 62:461.
Article Google Scholar
Wadstrom, T., and Smyth, T. (1974). In Isoelectric Focusing (U. P. Arbuthnott, ed.), Butterworths, London, p. 152.
Google Scholar
Weber, K., and Osborn, M. (1969). J. Biol. Chem. 244:4406.
PubMed CAS Google Scholar
Wyckoff, M., Rodbard, D., and Chrambach, A. (1976). Fed. Proc. 35:1383.
Google Scholar
Yadley, R. A., Rodbard, D., and Chrambach, A. (1973). Endocrinology 93:866.
Article PubMed CAS Google Scholar

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Authors and Affiliations

Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
A. Chrambach & D. Rodbard
Abteilung Molekulare Biologie, Max-Planck-Institut für Biophysikalische Chemie, Göttingen, German Federal Republic
T. M. Jovin
The Protein Laboratory, University of Copenhagen, Copenhagen, Denmark
P. J. Svendsen

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A. Chrambach
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D. Rodbard
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T. M. Jovin
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P. J. Svendsen
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Massachusetts Institute of Techonology, Cambridge, Massachusetts, USA
Nicholas Catsimpoolas

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Chrambach, A., Rodbard, D., Jovin, T.M., Svendsen, P.J. (1976). Analytical and Preparative Polyacrylamide Gel Electrophoresis. In: Catsimpoolas, N. (eds) Methods of Protein Separation. Biological Separations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9984-1_2

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DOI: https://doi.org/10.1007/978-1-4684-9984-1_2
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