Abstract
Although it is now established that LHRH and its agonistic analogues can exert direct effects on the gonads of several mammalian and non-mammalian species (Hsueh & Jones, 1981; Sharpe & Harmer, 1983), the physiological significance of these actions has not been resolved. In the rat testis, LHRH has direct effects only on the Leydig cells and these effects are believed to reflect the actions of an LHRH-like peptide which is secreted within the testis by the Sertoli cells (Sharpe et al. 1982a; Bhasin et al. 1983; Nagendranath et al. 1983; Sharpe & Harmar, 1983). In view of the critical dependence of normal Sertoli cell function on testosterone produced by the Leydig cells, such a line of communication has obvious physiological significance (see also Sharpe, 1983, 1984). Unfortunately, purification of ‘testicular LHRH’ has made poor progress (Bhasin et al. 1983; Sharpe & Harmar, 1983), so that the only line of investigation that has been possible is to explore the effects of synthetic LHRH and its analogues on the testis, and from the results obtained to infer the likely actions of the endogenous peptide. This paper summarizes our recent findings in this area which have been obtained using two different approaches: 1) by examining the effects of an LHRH agonist on Leydig cell function in vitro and in vivo, and 2) by examining the effects of an LHRH antagonist on Leydig cell function in vivo, as this compound should block any actions of endogenous ‘testicular LHRH’.
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References
Bhasin, S., Heber, D., Peterson, M., and Swerdloff, R., 1983, Partial isolation and characterization of testicular GnRHlike factors, Endocrinology, 112: 1144.
Bourne, G.A., Regiani, S., Payne, A.H., and Marshall, J.C., 1980, Testicular GnRH receptors-characterization and localization on interstitial tissue. J. Clin. Endocr. Metab., 51: 407.
Browning, J.Y., D’Agata, R., Steinberger, A., Grotjan, H.E. Jr., and Steinberger, E., 1983, Biphasic effect of GnRH and its agonist analog (HOE 766) on in vitro testosterone
production by purified rat Leydig cells, Endocrinology, 113: 985.
Hsueh, A.J.W., and Jones, P.B.C., 1981, Extrapituitary actions of gonadotropin-releasing hormone, Endocr. Rev., 2: 437.
Hunter, M.G., Sullivan, M.H.F., Dix, C.J., Aldred, L.F., and Cooke, B.A., 1982, Stimulation and inhibition by LHRH analogues of cultured rat Leydig cell function and lack of effect on mouse Leydig cells. Molec. Cell. Endocr., 27: 31.
Nagendranath, N., Jose, T.M., and Juneja, H.S., 1983, Bioassayable gonadotropin releasing hormone-like activity in the spent nutrient medium of rat Sertoli cells in primary cultures, Horm. Metab. Res., 15: 99.
Sharpe, R.M., 1983, Local control of testicular function, Qu. J. Exp. Physiol., 68: 265.
Sharpe, R.M., 1984, Intratesticular factors controlling testicular function. Biol. Reprod., (In press).
Sharpe, R.M., and Cooper, I., 1982, Stimulatory effect of LHRH and its agonists on Leydig cell steroidogenesis in vitro, Molec. Cell. Endocr., 26: 141.
Sharpe, R.M., Doogan, D.G., and Cooper, I., 1982b, Stimulation of Leyd,ig cell testosterone secretion in vitro and in vivo in hypophysectomized rats by an agonist of luteinizing hormone releasing hormone, Biochem. biophys. Res. Commun., 106: 1210.
Sharpe, R.M., Doogan, D.G., and Cooper, I., 1983a, Factors determining whether the direct effect of an LHRH agonist on Leydig cell function in vivo are stimulatory or inhibitory, Molec. Cell. Endocr., 32: 57.
Sharpe, R.M., Doogan, D.G., and Cooper, I., 1983b, Direct effects of an LHRH agonist on intratesticular levels of testosterone and interstitial fluid formation in intact male rats, Endocrinology, 113: 1306.
Sharpe, R.M., and Fraser, H.M., 1983, The role of LH in regulation of Leydig cell responsiveness to an LHRH agonist, Molec. Cell. Endocr., 33: 131.
Sharpe, R.M., Fraser, H.M., Cooper, I., and Rommerts, F.F.G., 1982a, The secretion, measurement and function of a testicular LHRH-like factor, Ann. N.Y. Acad. Sci., 383: 272.
Sharpe, R.M., and Harmar, A.J., 1983, The nature and biological actions of ‘testicular LHRH’, In: Hormones and Cell Regulation, vol. 7, (Eds. J.E. Dumont, J. Nunez and R.M. Denton ), pp. 217–230, Elsevier Biomedical Press, Amsterdam.
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Sharpe, R.M., Cooper, I. (1984). The Biological Actions of ‘Testicular LHRH’. In: McKerns, K.W., Naor, Z. (eds) Hormonal Control of the Hypothalamo-Pituitary-Gonadal Axis. Biochemical Endocrinology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9960-5_31
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DOI: https://doi.org/10.1007/978-1-4684-9960-5_31
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