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Microbiological Assay of Vitamin B-6 in Blood and Urine

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Methods in Vitamin B-6 Nutrition

Abstract

Although new, more precise techniques have been developed for the determination of vitamin B-6, the microbiological assay of this vitamin in blood and urine is still an important and valuable method. The assay organism used in our laboratory to measure vitamin B-6 is the yeast, Saccharomyces uvarum (formerly, S. carlsbergensis). Vitamin B-6 determination with this organism is simple, convenient, reproducible, and sensitive. In this paper, we will present the background for our choice of this organism and our procedure for determining plasma and urinary vitamin B-6.

Oregon Agricultural Experiment Station Technical Report No. 5574.

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© 1981 Springer Science+Business Media New York

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Miller, L.T., Edwards, M. (1981). Microbiological Assay of Vitamin B-6 in Blood and Urine. In: Leklem, J.E., Reynolds, R.D. (eds) Methods in Vitamin B-6 Nutrition. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9901-8_3

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  • DOI: https://doi.org/10.1007/978-1-4684-9901-8_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-9903-2

  • Online ISBN: 978-1-4684-9901-8

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