Skip to main content
SpringerLink
Log in

Some Effects That Mycoplasmas Have Upon Their Infected Host

  • Chapter
Mycoplasma Infection of Cell Cultures

Part of the book series: Cellular Senescence and Somatic Cell Genetics ((CSSCG,volume 3))

Abstract

Mycoplasma contamination of cultured cells was first reported in 1956 (1). A multitude of reports have followed this original observation, illustrating the frequent occurrence of these contaminants (reviewed in 2). Although mycoplasmas are common contaminants of tissue cultures many investigators regard them merely as a nuisance and have ignored their presence in experimental procedures since these organisms often exert no obvious effect upon the well-being of the infected cell population.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Robinson, L.B., R.H. Wichelbrausen, and B. Razinan. 1956. Contamination of human cell cultures by pleuropneumonia organisms. Science 124: 1147.

    Article  PubMed  CAS  Google Scholar 

  2. Stanbridge, E. 1971. Mycoplasmas and Cell Cultures. Bacteriol. Rev. 35: 206–227.

    PubMed  CAS  Google Scholar 

  3. Anderson, D.R., H.E. Hopps, M.F. Barile and B.C. Bernheim. 1965. Comparison of the ultrastructure of several rickettsiae, ornithosis virus, and mycoplasma in tissue culture. J. Bacteriol. 90: 1387–1404.

    PubMed  CAS  Google Scholar 

  4. Brown, S., M. Teplitz, and J.-P Revel. 1974. Interaction of mycoplasmas with cell cultures as visualized by electron microscopy. Proc. Nat. Acad. Sci. USA 71: 464–468.

    Article  PubMed  CAS  Google Scholar 

  5. Manchee, R., and D. Taylor-Robinson. 1969. Studies on the nature of receptors involved in attachment of tissue culture cells to mycoplasmas. Brit. J. Exp. Pathol. 50: 66–75

    CAS  Google Scholar 

  6. Sobeslaysky, O., B. Prescott, and R.M. Chanock. 1968. Adsorption of Mycoplasma pneumoniae to neuraminic acid receptors of various cells and possible role in virulence. J. Bacteriol. 96: 695–705.

    Google Scholar 

  7. Larin, N.M., N.V. Saxby, and D. Buggey. 1969. Quantitative aspects of Mycoplasma pneumoniae–cell relationships in cultures of lung diploid fibroblasts. J. Hyg. 67: 375–385.

    Article  CAS  Google Scholar 

  8. Fogh, J., and H. Fogh. 1967. Morphological and quantitative aspects of mycoplasma-human cell relationships. Proc. Soc. Exp. Biol. Med. 125: 423–430.

    PubMed  CAS  Google Scholar 

  9. Biberfeld, G. and E. Gronowicz. 1967. Mycoplasma pneumoniae is a polyclonal B-cell activator. Nature 261: 238–239.

    Article  Google Scholar 

  10. Callewaert, D.M., J. Kaplan, W.D. Peterson and J.W. Lightbody. 1975. Suppression of lymphocyte activation by a factor produced by Mycoplasma arginine. J. Immunol. 115: 1662–1664.

    PubMed  CAS  Google Scholar 

  11. Simberkoff, M.S., G.J. Thorbecke, and L. Thomas. 1969. Studies of PPLO infection. V. Inhibitors of lymphocyte mitosis and antibody formation by mycoplasmal extracts. J. Exp. Med. 129: 1163–1181.

    CAS  Google Scholar 

  12. Barile, M.F., and B.G. Levinthal. 1968. Possible mechanism for mycoplasma inhibition of lymphocyte transformation induced by phytohaemagglutinin. Nature 219: 751–752.

    Article  CAS  Google Scholar 

  13. Kraemer, P.M. 1964. Interaction of mycoplasma (PPLO) and murine lymphoma cell cultures. Prevention of cell lysis by arginine. Proc. Soc. Exp. Biol. Med. 115: 206–212.

    PubMed  CAS  Google Scholar 

  14. Butler, M. and R.H. Leach. 1964. A mycoplasma which induces acidity and cytopathic effect in tissue culture. J. Gen. Microbiol. 34: 285–294.

    Article  PubMed  CAS  Google Scholar 

  15. Kraemer, P.M., V. Defendi, L. Hayflick, and L. Manson. 1963. Mycoplasma (PPLO) strains with lytic activity for murine lymphoma cells in vitro. Proc. Soc. Exp. Biol. Med. 112: 381–387.

    PubMed  CAS  Google Scholar 

  16. Stanbridge, E., M. Önen, F.T. Perkins, and L. Hayflick. 1969. Karyological and morphological characteristics of human diploid cell strain WI-38 infected with mycoplasmas. Exp. Cell Res. 57: 397–410.

    Article  PubMed  CAS  Google Scholar 

  17. Jezequel, A.-M, M.M. Shreeve, and J.W. Steiner. 1967. Segregation of nucleolar components in mycoplasma-infected cells. Lab Invest. 16: 287–304.

    PubMed  CAS  Google Scholar 

  18. Kenny, G. 1973. Contamination of mammalian cells in culture by mycoplasmata. In: Contamination in Tissue Culture ( J. Fogh, Ed.) Academic Press, New York, pp. 108–131.

    Google Scholar 

  19. Schimke, R.T., C.M. Berlin, E.W. Sweeney and W.R. Carroll. 1966. The generation of energy by the arginine dihydrolase pathway in M. hominis. J. Biol. Chem. 241: 2228–2236.

    PubMed  CAS  Google Scholar 

  20. Smith, P.F. 1960. Amino acid metabolism of PPLO. Ann. N.Y. Acad. Sci. 79: 543–550.

    Article  PubMed  CAS  Google Scholar 

  21. Stanbridge, E.J., L. Hayflick, and F.T. Perkins. 1971. Modification of amino acid concentrations induced by mycoplasmas in cell culture medium. Nature New Biol. 232: 242–244.

    PubMed  CAS  Google Scholar 

  22. Plagemann, P.G., and D.P. Richeyn. 1974. Transport of nucleosides, nucleic acid bases, choline and glucose by animal cells in culture. Biochem. Biophys. Acta. 344: 263–305.

    Article  PubMed  CAS  Google Scholar 

  23. Perez, A.G., J.H. Kin, A.S. Gelbard, and B. Djordjevic. 1972. Altered incorporation of nucleic acid precursors by mycoplasmainfected mammalian cells in culture. Exp. Cell Res. 70: 301–310.

    Article  PubMed  CAS  Google Scholar 

  24. Schneider, E.L., and E.J. Stanbridge. 1975. Comparison of methods for the detection of mycoplasmal contamination of cell cultures: A review. In Vitro 11: 20–34.

    Article  PubMed  CAS  Google Scholar 

  25. Hellung-Larsen, P., and S. Frederiksen. 1976. Influence of mycoplasma infection on the incorporation of different precursors into RNA components of tissue culture cells. Exp. Cell Res. 99: 295–300.

    Article  PubMed  CAS  Google Scholar 

  26. Levine, E.M. 1972. Mycoplasma contamination of animal cell cultures: a simple rapid detection method. Exp. Cell Res. 74: 99–109.

    Article  PubMed  CAS  Google Scholar 

  27. Randall, C.C., L.G. Gafford, G.A. Gentry, and L.A. Lawson. 1965. Lability of host-cell DNA in growing cell cultures due to mycoplasma. Science 149: 1098–1099.

    Article  PubMed  CAS  Google Scholar 

  28. Russell, W.C. 1966. Alterations in the nucleic acid metabolism of tissue culture cells infected by mycoplasmas. Nature 212: 1537–1540.

    Article  PubMed  CAS  Google Scholar 

  29. Levine, E.M., I.G. Burleigh, C.W. Boone, and H. Eagle. 1967. An altered pattern of RNA synthesis in serially propagated human diploid cells. Proc. Nat. Acad. Sci. USA 57: 431–438.

    Article  PubMed  CAS  Google Scholar 

  30. Markov, G.G.., I. Bradvarova, A. Mintcheva, P. Petrov, N. Shiskov and R.G. Tsanev. 1969. Mycoplasma contamination of cell cultures: interference with 32P-labeling pattern of RNA. Exp. Cell REs. 57: 374–384.

    Article  PubMed  CAS  Google Scholar 

  31. Schneider, E.L., C.J. Epstein, W.L. Epstein, M. Betlach, and G. Abbo Halbasch. 1973. Detection of mycoplasma contamination in cultured human fibroblasts–comparison of biochemical and microbiological techniques. Exp. Cell Res. 79: 343–349.

    CAS  Google Scholar 

  32. Schneider, E.L., E.J. Stanbridge, and C.J. Epstein. 1974. Incorporation of 3H-uridine and 3H-uracil into RNA: a simple technique for the detection of mycoplasma contamination of cultured cells. Exp. Cell Res. 84: 311–318.

    Article  PubMed  CAS  Google Scholar 

  33. Stanbridge, E.J., J.A. Tischfield, and E.L. Schneider. 1975. Appearance of hypoxanthine guanine phosphoribosyl transferase activity as a consequence of mycoplasma contamination. Nature: 256: 329–331.

    Article  PubMed  CAS  Google Scholar 

  34. Miller, R.L. and F. Rapp. 1976. Distinguishing cytomegalovirus, mycoplasma, and cellular DNA polymerases. J. Virol. 20: 564–569.

    PubMed  CAS  Google Scholar 

  35. Mills, L.B., E.J. Stanbridge, D. Korn, and D. Sedwick. 1976 DNA polymerase from mycoplasmatales. Fed. Proc. 35: 1590.

    Google Scholar 

  36. Fry, M. and A. Weissbach. 1973. A new DNA dependent DNA polymerase from HeLa cell mitochondria. Biochem. 12: 3602–3608.

    Article  CAS  Google Scholar 

  37. Milunsky, A. and J.W. Littlefield. 1972. The prenatal diagnosis of inborn errors of metabolism. Ann. Rev. Med. 23: 57–76.

    Article  PubMed  CAS  Google Scholar 

  38. Fogh, J. and H. Fogh. 1965. Chromosome changes in PPLO-infected FL human amnion cells. Proc. Soc. Exp. Biol. Med. 119: 233–238.

    PubMed  CAS  Google Scholar 

  39. Paton, G.R., J.P. Jacobs and F.T. Perkins. 1965. Chromosome changes in human diploid cell cultures infected with mycoplasma. Nature 207: 43–45.

    Article  PubMed  CAS  Google Scholar 

  40. Aula, P. and W.W. Nichols. 1967. The cytogenetic effects of mycoplasma in human leucocyte cultures. J. Cell Physiol. 70: 281–290.

    Article  PubMed  CAS  Google Scholar 

  41. Spolsky, C.M., and J.M. Eisenstadt. 1972. Chloramphenicol resistant mutants of human HeLa cells. Febs Letters 25: 319–324.

    Article  PubMed  CAS  Google Scholar 

  42. McGarrity, G.J. and L.L. Coriell. 1971. Procedures to reduce contamination of cell cultures. In Vitro 6: 257–265.

    Article  PubMed  CAS  Google Scholar 

  43. McGarrity, G.J. 1976. Spread and control of mycoplasmal infection of cell cultures. In Vitro 12: 643–647.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. California College of Medicine, University of California, Irvine, Irvine, California, USA

    Eric J. Stanbridge

  2. Stanford University School of Medicine, Stanford, California, USA

    Claus-Jens Doersen

Authors
  1. Eric J. Stanbridge
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Claus-Jens Doersen
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institute for Medical Research, Camden, New Jersey, USA

    Gerard J. McGarrity  & Warren W. Nichols  & 

  2. National Institute on Aging, Bethesda, Maryland, USA

    Donald G. Murphy

Rights and permissions

Reprints and permissions

Copyright information

© 1978 Springer Science+Business Media New York

About this chapter

Cite this chapter

Stanbridge, E.J., Doersen, CJ. (1978). Some Effects That Mycoplasmas Have Upon Their Infected Host. In: McGarrity, G.J., Murphy, D.G., Nichols, W.W. (eds) Mycoplasma Infection of Cell Cultures. Cellular Senescence and Somatic Cell Genetics, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9874-5_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-9874-5_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-9876-9

  • Online ISBN: 978-1-4684-9874-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation