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Effects of Mycoplasmas on Lymphocyte Cell Cultures

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Mycoplasma Infection of Cell Cultures

Part of the book series: Cellular Senescence and Somatic Cell Genetics ((CSSCG,volume 3))

Abstract

The use of human lymphocyte cultures for cytogenetic studies was reported by Chrustschoff and Berlin in 1935 (1). Because of difficulties in propagation and maintenance of lymphocyte cell cultures, work was limited. Normal leukocytes do not ordinarily divide in peripheral blood although some do have mitotic potential. Early studies were designed to determine what factors could activate this latent potential. Plasma concentration, oxygen tension and carbon dioxide had only minor effects. In 1955, Rigas and Osgood reported the characteristics of phytohemagglutinin (PHA), a mitogen present in the red kidney bean, Phaseolus vulgaris (2). This plant extract was originally used to agglutinate erythrocytes in obtaining leukocytes from whole blood, and was shown to induce mitosis in leukocytes.

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Author information

Authors and Affiliations

  1. Department of Microbiology, Institute for Medical Research, Camden, New Jersey, USA

    Gerard J. McGarrity

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  1. Gerard J. McGarrity
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Editors and Affiliations

  1. Institute for Medical Research, Camden, New Jersey, USA

    Gerard J. McGarrity  & Warren W. Nichols  & 

  2. National Institute on Aging, Bethesda, Maryland, USA

    Donald G. Murphy

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McGarrity, G.J. (1978). Effects of Mycoplasmas on Lymphocyte Cell Cultures. In: McGarrity, G.J., Murphy, D.G., Nichols, W.W. (eds) Mycoplasma Infection of Cell Cultures. Cellular Senescence and Somatic Cell Genetics, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9874-5_12

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  • DOI: https://doi.org/10.1007/978-1-4684-9874-5_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-9876-9

  • Online ISBN: 978-1-4684-9874-5

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