Abstract
Electrophoretic analyses of the proteins synthesized by cultured human skin fibroblasts demonstrated that the major component released into the medium is a high molecular weight fucosylated glycoprotein (MFGP). Gel filtration chromatography under denaturing and reducing conditions indicated that MFGP has a molecular weight of approx. 250,000; but appears to behave as a disulphide-linked dimer when unreduced. MFGP is further distinguished as the major labelled macromolecule in the medium after incubation of fibroblasts with [35s]cysteine. The role of this glycoprotein is unknown but it bears a striking resemblance to a presumptive structural glycoprotein recently shown to be secreted by arterial smooth muscle cells in culture, and may also be related to a group of much studied cell surface glycoproteins.
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References
Anderson, J.C. (1976) Int. Rev. Connect. Tissue Res. 7, 251–322.
Lichtenstein, J.R., Byers, P.H., Smith, B.D. and Martin, G.R. (1975) Biochemistry, 14, 1589–1594.
Matalon, R. and Dorfman, A. (1969) Lancet, 2, 838–841
Kleinman, H.K., Silbert, J.E. and Silbert, C.K. (1975) Connect. Tissue Res. 4, 17–23.
Sear, C.H.J., Grant, Μ.E., Anderson, J.C. and Jackson, D.S. (1975) Biochem. Soc. Trans. 3, 138–140.
Gorham, L.W. and Waymouth, C. (1965) Proc. Soc. Exp. Biol. Med. 119, 287–290.
Laemmli, U.K. (1970) Nature, 227, 680–685.
Fairbanks, G., Steck, T.L. and Wallach, D.F.H. (1971) Biochemistry, 10, 2606–2617.
Harwood, R., Bhalla, A.K., Grant, M.E. and Jackson, D.S. (1974) Biochem. J. 148, 129–138.
Muir, L.W., Bornstein, P. and Ross, R. (1976) Europ. J. Biochem. 64, 105–114.
Sear, C.H.J., Grant, M.E. and Jackson, D.S. (1976) Biochem. Biophys. Res. Commun. 71, 379–384.
Weber, K. and Osborn, M. (1975) in The Proteins (H. Neurath and R.L. Hill, eds), vol. I, pp. 179–223, Academic Press, New York.
Harwood, R., Merry, A.H., Woolley, D.E., Grant, M.E. and Jackson, D.S. (1976) Biochem. J. submitted for publication.
Vaheri, Α., Ruoslahti, E., Linder, Ε., Wartiovaara, J., Keski-oja, J., Kuusela, P. and Saksela, O. (1976) J. Supramol. Struct. 4, 63–70.
Hynes, R.O. (1976) Biochim. Biophys. Acta, 458, 73–107.
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© 1977 Plenum Press, New York
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Sear, C.H.J., Grant, M.E., Jackson, D.S. (1977). Studies on the Major Fucosylated Glycoprotein Released into the Medium by Cultured Human Skin Fibroblasts. In: Sandberg, L.B., Gray, W.R., Franzblau, C. (eds) Elastin and Elastic Tissue. Advances in Experimental Medicine and Biology, vol 79. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9093-0_33
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DOI: https://doi.org/10.1007/978-1-4684-9093-0_33
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