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Immobilized Artificial Membrane Chromatography

Initial Studies Using Monomyristoylphosphatidylcholine as a Detergent for Solubilizing and Purifying Membrane Proteins

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Book cover Modern Phytochemical Methods

Part of the book series: Recent Advances in Phytochemistry ((RAPT,volume 25))

Abstract

Immobilized artificial membranes (IAM) are solid-phase-membrane-mimetics.1 Synthesis of IAM particles entails bonding cell membrane lipid molecules to solid surfaces at high molecular surface densities.1–4 Thus IAM surfaces are intended to mimic the lipid environment of cell membranes and consequently the initial applications of IAM particles relate to endogenous solute—membrane interactions. Non-chromatographic applications of IAM include reconstitution of phospholipase D (unpublished observation), and the correlation of drug-binding to IAM with drug-transport through human skin.5 Chromatographic applications include the purification of cytochrome P-450 (submitted for publication and also refs.6,7), and the purification of other membrane proteins.8 Although both non-chromatographic and chromatographic applications are evolving, the most useful application (s) of IAM will be for the purification of membrane proteins.

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Authors and Affiliations

  1. Department of Medicinal Chemistry and Pharmacognosy, School of Pharmacy, Purdue University, West Lafayette, IN, 47907, USA

    Whi-Gun Chae, Congwen Luo, Dong Mi Rhee & Charles Pidgeon

  2. Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA

    Christian R. Lombardo & Phil Low

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  1. Whi-Gun Chae
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  2. Congwen Luo
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  3. Dong Mi Rhee
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  4. Christian R. Lombardo
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  5. Phil Low
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  6. Charles Pidgeon
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Editor information

Editors and Affiliations

  1. Louisiana State University, Baton Rouge, Louisiana, USA

    Nikolaus H. Fischer

  2. University of British Columbia, Vancouver, British Columbia, Canada

    Murray B. Isman

  3. Reed College, Portland, Oregon, USA

    Helen A. Stafford

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© 1991 Plenum Press, New York

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Chae, WG., Luo, C., Rhee, D.M., Lombardo, C.R., Low, P., Pidgeon, C. (1991). Immobilized Artificial Membrane Chromatography. In: Fischer, N.H., Isman, M.B., Stafford, H.A. (eds) Modern Phytochemical Methods. Recent Advances in Phytochemistry, vol 25. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9060-2_5

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  • DOI: https://doi.org/10.1007/978-1-4684-9060-2_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-9062-6

  • Online ISBN: 978-1-4684-9060-2

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