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Biosynthesis of Factor XIII A and B Subunits

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Advances in Post-Translational Modifications of Proteins and Aging

Part of the book series: Advances in Experimental Medicine and Biology ((NATO ASI F,volume 231))

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Abstract

Factor XIIIa (FXIIIa), the blood coagulation enzyme, functions as a transglutaminase to covalently stabilize fibrin clots by introducing γ-glutamyl-ε-lysyl peptide bonds between fibrin molecules (for detailed reviews, see references 1 and 2). The FXIII proenzyme exists in two zymogenic forms.3 Extracellular or plasma FXIII is composed of two subunits, A(Mr =80,500)4 and B(M =76,500),5 associated non-covalently as a tetrameric complex, A2B2.6,7 Intracellular FXIII is a dimer of only A subunits, A2.6 The A chains in both zymogens are identical and contain the active center sulfhydryl group.7,8 Upon activation by thrombin in the presence of calciurn ions, both zymogens give rise to the same enzymatic activity, FXIIIa.8,9

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References

  1. Lorand L, Losowsky MS, Miloszewski KJM: Human factor XIII: Fibrin-stabilizing factor. Prog Hemost Thromb 5:245, 1980

    PubMed  CAS  Google Scholar 

  2. McDonagh J: Structure and function of factor XIII, in Coleman RW, Hirsh J, Marder VJ, Salzman EW (eds): Hemostasis and Thrombosis, Basic Principles and Clinical Practice. Philadelphia, Lippincott, 1982, p 164

    Google Scholar 

  3. Dvilansky A, Britten AFH, Loewy AG: Factor XIII assay by an isotope method. I. Factor XIII (transamidase) in plasma, serum, leukocytes, erythrocytes and platelets and evaluation of screening tests of clot solubility. Br J Haematol 18:399, 1970

    Article  PubMed  CAS  Google Scholar 

  4. Ichinose A, Hendrickson LE, Fujikawa K, Davie EW: Amino acid sequence of the a subunit of human factor XIII. Biochemistry 25:6900, 1986

    Article  PubMed  CAS  Google Scholar 

  5. Ichinose A, McMullen BA, Fujikawa K, Davie EW: Amino acid sequence of the b subunit of human factor XIII, a protein composed of ten repetitive segments. Biochemistry 25:4633, 1986

    Article  PubMed  CAS  Google Scholar 

  6. Schwartz ML, Pizzo SV, Hill RL, McKee PA: Human factor XIII from plasma and platelets: Molecular weights, subunit structure, proteolytic activation and crosslinking of fibrinogen and fibrin. J Biol Chem 248:1395, 1973

    PubMed  CAS  Google Scholar 

  7. Chung SI, Lewis MS, Folk JE: Relationships of the catalytic properties of human plasma and platelet transglutaminases (activated blood coagulation factor XIII) to their subunit structure. J Biol Chem 249:940, 1974

    PubMed  CAS  Google Scholar 

  8. Curtis CG, Brown KL, Credo RB, Domanik RA, Gray A, Stenberg P, Lorand L: Calcium dependent unmasking of active center cysteine during activation of fibrin stabilizing factor. Biochemistry 13:3774, 1974

    Article  PubMed  CAS  Google Scholar 

  9. Freyssinet JM, Lewis BA, Holbrook JJ, Shore JD: Protein-protein interactions in blood clotting. The use of polarization of fluorescence to measure the dissociation of plasma factor XIIIa. Biochem J 169:403, 1978

    PubMed  CAS  Google Scholar 

  10. Nagy JA, Henriksson P, McDonagh J: Biosynthesis of factor XIII B subunit by human hepatoma cell lines. Blood 68:1272, 1986

    PubMed  CAS  Google Scholar 

  11. Kradin RL, Lynch GW, Kurnick JT, Erikson M, Colvin RB, McDonagh J: Factor XIII A is synthesized and expressed on the surface of U937 cells and alveolar macrophages. Blood 69:778, 1987

    PubMed  CAS  Google Scholar 

  12. Kiesselback TH, Wagner RH: Demonstration of factor XIII in human megakaryocytes by a fluorescent antibody technique. Ann NY Acad Sci 202:318, 1972

    Article  Google Scholar 

  13. Chung SI: Multiple molecular forms of transglutaminases in human and guinea pig, in Markert CL (ed): Isozymes, vol 1. Orlando, FL, Academic, 1975, p 259

    Chapter  Google Scholar 

  14. Bohn H, Haupt H, Kranz T: Die molekulare Struktur der fibrinstabilisierenden Faktoren des Menschen. Blut 25:235, 1972

    Article  PubMed  CAS  Google Scholar 

  15. Colvin R, Lynch G, McDonagh J, Erikson M, Karnick J, Power K, Kradin R: Factor XIII A subunit is synthesized and expressed by macrophages. Fed Proc 44:906, 1985

    Google Scholar 

  16. Muszbek L, Adany R, Szeyedi G, Polgar J, Kavai M: Factor XIII of blood coagulation in human monocytes. Thromb Res 37:401, 1985

    Article  PubMed  CAS  Google Scholar 

  17. Henriksson P, Becker S, Lynch G, McDonagh J: Identification of intracellular factor XIII in human monocytes and macrophages. J Clin Invest 76:528, 1985

    Article  PubMed  CAS  Google Scholar 

  18. Weisberg LJ, Shiu DT, Greenberg CS, Kan YW, Shuman MA: Localization of the gene for coagulation factor XIII a-chain to chromosome 6 and identification of sites of synthesis. J Clin Invest 79:649, 1987

    Article  PubMed  CAS  Google Scholar 

  19. Leu RW, Herriot MJ, Moore PE, Orr GR, Birkbichler P: Enhanced transglutaminase activity associated with macrophage activation. Exp Cell Res 141:191, 1982

    Article  PubMed  CAS  Google Scholar 

  20. Murtaugh MP, Mehta K, Johnson J, Myers M, Juliano RL, Davies PJA: Induction of tissue transglutaminase in mouse peritoneal macrophages. J Biol Chem 258:11074, 1983

    PubMed  CAS  Google Scholar 

  21. Murtaugh M, Arend WP, Davies PJ: Induction of tissue transglutaminase in human peripheral blood monocytes. J Exp Med 159:114, 1984

    Article  PubMed  CAS  Google Scholar 

  22. Mehta K, Lopez-Berestein G, Moore WT, Davies PJ: Interferon-γ requires serum retinoids to promote the expression of tissue transglutaminase in cultured human blood monocytes. J Immunol 134:2053, 1985

    PubMed  CAS  Google Scholar 

  23. Workman EF Jr, Lundblad RL: The role of the liver in the biosynthesis of the non-vitamin K-dependent clotting factors. Semin Thromb Hemost 4:15, 1977

    PubMed  CAS  Google Scholar 

  24. Lee SY, Chung SI: Biosynthesis and degradation of plasma protransglutaminase (factor XIII). Fed Proc 35:1486, 1976

    Google Scholar 

  25. Ikematsu S: An approach to the metabolism of factor XIII. Acta Haematol Jpn 44:1499, 1981

    CAS  Google Scholar 

  26. Aden DP, Fogel A, Plotkin S, Damjanov I, Knowles BB: Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line. Nature 282:615, 1979

    Article  PubMed  CAS  Google Scholar 

  27. Knowles BB, Howe CC, Aden DP: Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209:497, 1980

    Article  PubMed  CAS  Google Scholar 

  28. Fair DS, Plow EF: Synthesis and secretion of the fibrinolytic components, including α2-antiplasmin, by a human hepatoma cell line. J Lab Clin Med 101:372, 1983

    PubMed  CAS  Google Scholar 

  29. Levin EG, Fair DS, Loskutoff DJ: Human hepatoma cell line plasminogen activator. J Lab Clin Med 102:500, 1983

    PubMed  CAS  Google Scholar 

  30. Yu S, Sher B, Kudryk B, Redman CM: Intracellular assembly of human fibrinogen. J Biol Chem 258:13407, 1983

    PubMed  CAS  Google Scholar 

  31. Yu S, Sher B, Kudryk B, Redman CM: Fibrinogen precursors. Order of assembly of fibrinogen chains. J Biol Chem 259:10574, 1984

    PubMed  CAS  Google Scholar 

  32. Wilson DB, Salem HH, Mruk JS, Maruyama I, Majerus PW: Biosynthesis of coagulation factor V by a human hepatocellular carcinoma cell line. J Clin Invest 73:654, 1984

    Article  PubMed  CAS  Google Scholar 

  33. Fair DS, Bahnak BR: Human hepatoma cells secrete single chain factor X, prothrombin and antithrombin III. Blood 64:194, 1984

    PubMed  CAS  Google Scholar 

  34. Morris KM, Aden DP, Knowles BB, Colten HR: Complement biosynthesis by the human hepatoma-derived cell line Hep G2. J Clin Invest 70:906, 1982

    Article  PubMed  CAS  Google Scholar 

  35. Goldberger G, Arnaout MA, Aden D, Kay R, Rits M, Colten HR: Biosynthesis and postsynthetic processing of human C3b/C4b inactivator (factor I) in three hepatoma cell lines. J Biol Chem 259:6492, 1984

    PubMed  CAS  Google Scholar 

  36. Alexander JJ, Bey EM, Geddes EW, Lecatsas G: Establishment of a continuously growing cell line from primary carcinoma of the liver. S Afr Med J 50:2124, 1974

    Google Scholar 

  37. Lorand L, Campbell-Wilkes LK, Cooperstein L: A filter paper assay for transamidating enzymes using radioactive amine substrates. Anal Biochem 50:623, 1972

    Article  PubMed  CAS  Google Scholar 

  38. Godal HC, Gravem K, Brossted F, Nyvold N: Quantitation of factor XIII by SDS Polyacrylamide gel electrophoresis. Thromb Res 35:577, 1984

    Article  PubMed  CAS  Google Scholar 

  39. Henriksson P, McDonagh J: Factor XIII activation and interactions, in Egbring R, Klingemann HG (eds): Factor XIII and Fibronectin. Marburg, Germany, Med Verlagsgesellschaft, 1983, p 1

    Google Scholar 

  40. Simon D, Knowles BB: Hepatocellular carcinoma cell line and peripheral blood lymphocytes from the same patient contain common chromosomal alterations. Lab Invest 55:657, 1986

    PubMed  CAS  Google Scholar 

  41. Grundmann U, Amann E, Zettlmeissl G, Kupper HA: Characterization of cDNA coding for human factor XIIIa. Proc Natl Acad Sci 83:8024, 1986

    Article  PubMed  CAS  Google Scholar 

  42. Skrzynia C., Reisner HM, McDonagh J: Characterization of the catalytic subunit of factor XIII by radioimmunoassay. Blood 60:1089, 1982

    PubMed  CAS  Google Scholar 

  43. Gates GA, Henley KS, Pollard HM, Schmidt E, Schmidt FW: The cell populations of human liver. J Lab Clin Med 57:182, 1961

    PubMed  CAS  Google Scholar 

  44. Grinnell F: Fibronectin and wound healing. J Cell Biochem 26:107, 1984

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Pathology, Beth Israel Hospital, Boston, MA, USA

    Janice A. Nagy, Richard L. Kradin & Jan McDonagh

  2. Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA

    Janice A. Nagy, Richard L. Kradin & Jan McDonagh

Authors
  1. Janice A. Nagy
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  2. Richard L. Kradin
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  3. Jan McDonagh
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Editor information

Editors and Affiliations

  1. University of Naples, Naples, Italy

    Vincenzo Zappia , Patrizia Galletti  & Raffaele Porta ,  & 

  2. University of Texas Health Science Center, Houston, Texas, USA

    Finn Wold

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© 1988 Springer Science+Business Media New York

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Nagy, J.A., Kradin, R.L., McDonagh, J. (1988). Biosynthesis of Factor XIII A and B Subunits. In: Zappia, V., Galletti, P., Porta, R., Wold, F. (eds) Advances in Post-Translational Modifications of Proteins and Aging. Advances in Experimental Medicine and Biology, vol 231. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9042-8_3

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  • DOI: https://doi.org/10.1007/978-1-4684-9042-8_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-9044-2

  • Online ISBN: 978-1-4684-9042-8

  • eBook Packages: Springer Book Archive

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