Identification of Lysis-Relevant Molecules on the Surface of CTL: Primary Screening of Monoclonal Antibodies for the Capacity to Block Cytolysis by Cloned CTL Lines
Cytolytic T lymphocytes (CTL) couple antigen recognition with cytolysis of the target cell. The recognition (conjugate formation) and lethal hit components can be distinguished operationally. Both the antigen recognition structure and the lethal hit activity are unidentified. It is possible that the antigen recognition structure on CTL is modified so as to possess this lethal hit activity (1). Implicit in the work described here is the not unreasonable assumption that the molecule(s) responsible for recognition and for the lethal hit exist on the surface of the cytolytic T lymphocyte.
KeywordsBlocking Activity Supernatant Fluid Target Cell Ratio Blocking Assay Phenol Blue
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- 1.Geiger, B., K.L. Rosenthal, J. Klein, R.M. Zinkernagel, and S.J. Singer. Selective and unidirectional membrane redistribution of an H-2 antigen with an antibody-clustered viral antigen: Relationship to mechanisms of cytotoxic T-cell interactions. Proc. Natl. Acad. Sci. 76:4603. (1979)PubMedCrossRefGoogle Scholar
- 8.Wall, K.A., and F.W. Fitch. Effects of concanavalin A pre- treatment on cloned cytolytic cells. (This volume). (1982)Google Scholar
- 10.Pierres, M., C. Goridis, and P. Golstein. Inhibition of murine T cell-mediated cytolysis and T cell prliferation by a rat monclonal antibody immunoprecipitating two lymphoid cell surface polypeptides of 94,000 and 180,000 molecular weight. Eur. J. Immunol. In press. (1981)Google Scholar
- 11.Trowbridge, I.S., and M.B. Omary. J. Exp. Med. In press. (1981)Google Scholar