The Differential Effects of Distinct Cytolysis-Inhibiting Monoclonal Antibodies on Growth and on Cytolytic Activity of T Cell Clones
The analysis of the involvement of cell-surface structures of cytotoxic T lympocytes (CTL) in lytic interactions with target cells has been approached in recent years by the use of antibodies capable of inhibiting cytolysis. Among antibodies found to inhibit cytolysis via their binding to the effector cells, were allogeneic anti- Lyt2 antibodies (2–6) and a xenogeneic rat anti-mouse antiserum (7). Unlike these antibodies which blocked cytolysis in the absence of added complement, antibodies raised in syngeneic mice, directed at T cell blast-specific structures possibly involved in antigen recognition, required additional complement treatment to eliminate the CTL (8,9). More recently, a systematic search has been made for cytolysis-inhibiting monoclonal antibodies (mAb) secreted by hybridoma cells obtained after fusion of rat cells immunized with mouse T cell populations (1,10–14), This has resulted in the production of mAb directed at two types of cell surface structures: (a) the Lyt2 molecule (1,10,11) and its associated Lyt3 molecule (10,11), and (b) a glycoprotein composed of 180 and 95K polypeptides defined independently by mAb LFA-1 (12,13), mAb described by Fitch and colleagues (14), and by mAb H35-89.9 described by Pierres and colleagues (1).
KeywordsCytolytic Activity 51Cr Release Allelic Form Mixed Lymphocyte Culture Reciprocal Dilution
Unable to display preview. Download preview PDF.
- 1.Plerres, M., Goridis, C., and P. Golstein. Inhibition of murine T cell ediated cytolysis and T cell proliferation by a rat monoclonal antibody immunoprecipitating two lymphoid cell surface polypeptides of 94,000 and 180,000 molecular weight. Eur. J. Immunol, (in presss)(1981).Google Scholar
- 5.Fan, J., Ahmed, A., and B. Bonavida. Studies on the induction and expession of T cell-mediated immunity. X. Inhibition by Lyt-2,3 antisera of cytotoxic T lymphocyte-mediated antigen- specific and non-specific cytotoxicity: evidence for the blocking of the binding between T Ijmiphocytes and target cells and not the post-binding cytolytic steps. J. Immunol. 125: 2444 (1980).PubMedGoogle Scholar
- 9.Krammer, P. The T cell receptor problem. Curr. Top. Microbiol. and Immunol. (1980).Google Scholar
- 13.Davignon, D., Martz, E., Reynolds, T., Kurzinger, K., and T.A. Springer. Monoclonal antibody to a novel lymphocyte function- associated antigen (LFA) (l). Mechanism of blockade of T lymphocyte mediated killing and effects on other T and B Ijnnphocyte functions. J. Immunol. 127:590 (1981).PubMedGoogle Scholar
- 14.Fitch, F.W. et al, this volume.Google Scholar
- 15.Gillis, S., Ferm, M.M., Oe, W., and K.A. Smith. T cell growth factor: parameters of production and a quantitative microassay for activity. J, Immunol. 120:2027 (1978).Google Scholar
- 16.Nabholz, M., Engers, H.D., Collavo, D., and M. North. Cloned T cell lines with specific cytolytic activity. Curr. Top. Microbiol, and Immunol. 81:176 (1978).Google Scholar
- 19.Schmitt-Verhulst, A.M., Albert, F., Guimezanes, A., and M. Buferne. Antigenic and genetic parameters in the stimulation and in the lytic phases of anti-hapten + self cytotoxic T cells and their derived clones: role of the T helper cell. J. Sup- ramol. Structure and Cell. Biochem. (in press)(1981).Google Scholar
- 28.Klein, J., Flaherty, L., Vandeberg, J.L., and D.C. Schreffler. H-2 haplotypes, genes, regions, and antigens: first lising. Immunogenetics 6:489 0978).Google Scholar