The Differential Effects of Distinct Cytolysis-Inhibiting Monoclonal Antibodies on Growth and on Cytolytic Activity of T Cell Clones

  • Anne-Marie Schmitt-Verhulst
  • Pierre Golstein
  • Michel Buferne
  • Michel Pierres
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 146)


The analysis of the involvement of cell-surface structures of cytotoxic T lympocytes (CTL) in lytic interactions with target cells has been approached in recent years by the use of antibodies capable of inhibiting cytolysis. Among antibodies found to inhibit cytolysis via their binding to the effector cells, were allogeneic anti- Lyt2 antibodies (2–6) and a xenogeneic rat anti-mouse antiserum (7). Unlike these antibodies which blocked cytolysis in the absence of added complement, antibodies raised in syngeneic mice, directed at T cell blast-specific structures possibly involved in antigen recognition, required additional complement treatment to eliminate the CTL (8,9). More recently, a systematic search has been made for cytolysis-inhibiting monoclonal antibodies (mAb) secreted by hybridoma cells obtained after fusion of rat cells immunized with mouse T cell populations (1,10–14), This has resulted in the production of mAb directed at two types of cell surface structures: (a) the Lyt2 molecule (1,10,11) and its associated Lyt3 molecule (10,11), and (b) a glycoprotein composed of 180 and 95K polypeptides defined independently by mAb LFA-1 (12,13), mAb described by Fitch and colleagues (14), and by mAb H35-89.9 described by Pierres and colleagues (1).


Cytolytic Activity 51Cr Release Allelic Form Mixed Lymphocyte Culture Reciprocal Dilution 
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  1. 1.
    Plerres, M., Goridis, C., and P. Golstein. Inhibition of murine T cell ediated cytolysis and T cell proliferation by a rat monoclonal antibody immunoprecipitating two lymphoid cell surface polypeptides of 94,000 and 180,000 molecular weight. Eur. J. Immunol, (in presss)(1981).Google Scholar
  2. 2.
    Shinohara, N., and D.H. Sachs, mouse alloantibodies capable of blocking cytotoxic T cell function. I. Relationship between the antigen reactive with blocking antibodies and the Lyt-2 locus. J. Exp. Med. 150:432 (1979).PubMedCrossRefGoogle Scholar
  3. 3.
    Shinohara, N., Hammerling, U., and D.H. Sachs. Mouse alloantibodies capable of blocking cytotoxic T cell function. II. Further study on the relationship between the blocking antibodies and the products of the Lyt-2 locus. Eur. J. Immunol. 10:589 (1980).PubMedCrossRefGoogle Scholar
  4. 4.
    Nakayama, E., Dippold, W., Shiku, H., Oettgen, H.F., and L.J. Old. Alloantigen-induced T-cell proliferation: Lyt phenotype of responding cells and blocking of proliferation by Lyt anti- sera. Proc. Natl. Acad. Sci. USA 77:2890 (1980).PubMedCrossRefGoogle Scholar
  5. 5.
    Fan, J., Ahmed, A., and B. Bonavida. Studies on the induction and expession of T cell-mediated immunity. X. Inhibition by Lyt-2,3 antisera of cytotoxic T lymphocyte-mediated antigen- specific and non-specific cytotoxicity: evidence for the blocking of the binding between T Ijmiphocytes and target cells and not the post-binding cytolytic steps. J. Immunol. 125: 2444 (1980).PubMedGoogle Scholar
  6. 6.
    Hollander, N., Pillemer, E., and I.L. Weissman. Blocking effect of Lyt-2 antibodies on T cell function. J. Exp. Med. 152:674 (1980).PubMedCrossRefGoogle Scholar
  7. 7.
    Hiserodt, J.C., and B. Bonavida. Studies on the maturation and expression of T cell-mediated immunity. XI. Inhibition of the “lethal hit” in T cell-mediated cytotoxicity by heterologous rat antiserum made against alloimmune T lymphocytes. J. Immunol. 126:256 (1981).PubMedGoogle Scholar
  8. 8.
    Binz, H., Frischknecht, Shen, F.W., and H. Wigzell. Idiotypic determinants on T-cell subpopulations. J. Exp. Med. 149:910 (1979).PubMedCrossRefGoogle Scholar
  9. 9.
    Krammer, P. The T cell receptor problem. Curr. Top. Microbiol. and Immunol. (1980).Google Scholar
  10. 10.
    Sarmiento, M., Glasebrook, A.L., and F.W. Fitch. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt-2 antigen block T cell- mediated cytolysis in the absence of complement. J. Immunol. 125:2665 (1980).PubMedGoogle Scholar
  11. 11.
    Ledbetter, J.A., Seaman, W.E., Tse, T.T., and L.A. Herzenberg. Lyt-2 and Lyt-3 antigens are on two different polypeptide sub- units linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity. J. Exp. med. 153:1503 (1981).PubMedCrossRefGoogle Scholar
  12. 12.
    Kurzinger, K., Reynolds, T., Germain, R.N., Davignon, D., Martz, E., and T.A. Springer. A novel lymphocyte function-associated antigen (LFA-1): cellular distribution, quantitative expression and structure. J. Immunol. 127:596 (1981).PubMedGoogle Scholar
  13. 13.
    Davignon, D., Martz, E., Reynolds, T., Kurzinger, K., and T.A. Springer. Monoclonal antibody to a novel lymphocyte function- associated antigen (LFA) (l). Mechanism of blockade of T lymphocyte mediated killing and effects on other T and B Ijnnphocyte functions. J. Immunol. 127:590 (1981).PubMedGoogle Scholar
  14. 14.
    Fitch, F.W. et al, this volume.Google Scholar
  15. 15.
    Gillis, S., Ferm, M.M., Oe, W., and K.A. Smith. T cell growth factor: parameters of production and a quantitative microassay for activity. J, Immunol. 120:2027 (1978).Google Scholar
  16. 16.
    Nabholz, M., Engers, H.D., Collavo, D., and M. North. Cloned T cell lines with specific cytolytic activity. Curr. Top. Microbiol, and Immunol. 81:176 (1978).Google Scholar
  17. 17.
    von Boehmer, H., and W. Hass. H-2 restricted cytolytic and non-cytolytic T cell clones: isolation specificity and functional analysis. Immunol. Rev. 54:27 (1981).CrossRefGoogle Scholar
  18. 18.
    Glasebrook, A.L., Sarmiento, M., Loken, M.R., Dialynas, D.P., Quintans, J., Eisenberg, L., Lutz, C.T., Wilde, D., and F.W. Fitch. Murine T lymphocyte clones with distinct immunological functions. Immunol. Rev. 54:225 (1981).PubMedCrossRefGoogle Scholar
  19. 19.
    Schmitt-Verhulst, A.M., Albert, F., Guimezanes, A., and M. Buferne. Antigenic and genetic parameters in the stimulation and in the lytic phases of anti-hapten + self cytotoxic T cells and their derived clones: role of the T helper cell. J. Sup- ramol. Structure and Cell. Biochem. (in press)(1981).Google Scholar
  20. 20.
    Lemonnier, F., Neuport-Sautes, C., Korilsky, F.M., and P. Demant. Relationships between private and public H-2 specificities on the cell surface. Immunogenetics 2:517 (1975).CrossRefGoogle Scholar
  21. 21.
    Hansen T.H., Ozato, K., Melino, MR., Coligan, J.E., Kindt, T.J., Jandinsky, J.J., and D.H. Sachs. Immunochemical evidence in two haplotypes for at least three D region-encoded molecules D, L and R. J. Immunol. 126:1713 (1981).PubMedGoogle Scholar
  22. 22.
    Lutz, C.T., Glasebrook, A.L., and F.W. Fitch. Alloreactive cloned T cell lines. IV. Interaction of alloantigen and T cell growth factors (TCGF) to simulate cloned cytolytic T lymphocytes. J. Immunol. 127:391 (1981).PubMedGoogle Scholar
  23. 23.
    Dialynas, D.P., Loden, M.R., Glasebrook, A.L., and F.W. Fitch. Lyt-2-/Lyt-3+ variants of a cloned cytolytic T cell ine lack an antigen receptor functional in cytolysis. J. Exp. Med. 153:595 (1981).PubMedCrossRefGoogle Scholar
  24. 24.
    Swain, S.L., Dennert, G., Wormsley, S., and R.W. Dutton. The Lyt phenotype of a long-term allospecific T cell line. Both helper and killer activities to I-A are mediated by Lyt-1 cells. Eur. J. Immunol. 11:175 (1981).PubMedCrossRefGoogle Scholar
  25. 25.
    MacDonald, H.R., Thiernesse, N., and J.C. Cerottini. Inhibition of T cell-mediated cytolysis by monoclonal antibodies directed against Lyt-2: heterogeneity of inhibition at the clonal level. J. Immunol. 126:1671 (1981).PubMedGoogle Scholar
  26. 26.
    Nabholz, M., Cianfriglia, M., Acuto, O., Conzelmann, A., Haas, W., Boehmer, H.V., MacDonald, H.R., Pohllt, H, and J.P. Johnson. Cytolytically active murine T-cell hybrids. Nature 287:437 (1980).PubMedCrossRefGoogle Scholar
  27. 27.
    Kaufmann, Y., Berke, G., and Z. Eshhar. Cytotoxic T lymphocyte hybridomas which mediate specific tumor cell lysis in vitro. Proc. Natl. Acad. Sci. USA 78:2502 (1981).PubMedCrossRefGoogle Scholar
  28. 28.
    Klein, J., Flaherty, L., Vandeberg, J.L., and D.C. Schreffler. H-2 haplotypes, genes, regions, and antigens: first lising. Immunogenetics 6:489 0978).Google Scholar
  29. 29.
    Fisher-Lindhal, K., and B. Hausmann. Qed-1: a target for unrestricted killing by T cells. Eur. J. Immunol. 10:281 (1980).CrossRefGoogle Scholar
  30. 30.
    Festenstein, H. Immunogenetic and biological aspects of in vitro lymphocyte allotransformatin (MLR) in the mouse. Transplant. Rev. 15:62 (1973).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Anne-Marie Schmitt-Verhulst
    • 1
  • Pierre Golstein
    • 1
  • Michel Buferne
    • 1
  • Michel Pierres
    • 1
  1. 1.Centre d’ImmunologieINSERM-CNRS de Marseille-LuminyMarseille Cedex 9France

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