Cytolytic T Lymphocyte Recognition of Subcellular Antigen

  • Matthew F. Mescher
  • Steven P. Balk
  • Steven J. Burakoff
  • Steven H. Herrmann
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 146)


Specific antigen recognition is required to stimulate generation of a cytolytic T lymphocyte (CTL1) response and to allow the resulting effector CTLs to bind and lyse the target cells. Reliable assays allow in vitro assessment of stimulation of a primary and secondary response and of the effector-target interaction. The antigens required to trigger generation of a response are the same as those required to allow binding and lysis by the effector CTL.


Major Histocompatibility Complex Spleen Cell Conjugate Formation Antigen Density Purify Plasma Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. (1).
    Klein, J. 1979. The major histocompatibility complex of the mouse. Science 203:516.PubMedCrossRefGoogle Scholar
  2. (2).
    Nathenson, S.G., Uehara, H., and Ewenstein, B.M. 1981. Primary structural analysis of the transplantation antigens of the murine H-2 major histocompatibility complex. Ann. Rev. Biochem. 50:1025.PubMedCrossRefGoogle Scholar
  3. (3).
    Matzinger, P., and Bevan, M.J. 1977. Hypothesis. Why do so many lymphocytes respond to major histocompatibility antigens. Cell. Immunol. 29:1.PubMedCrossRefGoogle Scholar
  4. (4).
    Kinsky, S.C., and Nicolotti, R.A. 1977. Immunological properties of model membranes. Ann. Rev. Biochem. 46:49.PubMedCrossRefGoogle Scholar
  5. (5).
    Engers, H.D., Thomas, K., Cerottini, J.C., and Brunner, K.T. 1975. Generation of cytotoxic T cells in vitro. V. Response of normal and immune spleen cells to subcellular alloantigen. J. Immunol. 115;356.PubMedGoogle Scholar
  6. (6).
    Wagner, H., Hess, M., Feldmann, M., and Rollinghoff, M. 1976. Secondary cytotoxic allograft responses in vitro. III. The immunogenicity of allogeneic membrane fragments. Transplantation 21:282.PubMedCrossRefGoogle Scholar
  7. (7).
    Häyry, P., and Anderson, L.C. 1976. Generation of T memory cells in one-way mixed lymphocyte culture. IV. Primary and secondary responses to soluble and insoluble membrane preparations and to ultraviolet light inactivated stimulator cells. Scand. J. Immunol. 5:391.PubMedCrossRefGoogle Scholar
  8. (8).
    Lemonnier, F., Mescher, M.F., Sherman, L., and Burakoff, S. 1978. The induction of cytolytic T lymphocytes with purified plasma membranes. J. Immunol. 120:1114.PubMedGoogle Scholar
  9. (9).
    Todd, R.F., Stuiting, R.D., and Amos, D.B. 1975. Lymphocyte- mediated cytolysis of allogeneic tumor cells in vitro. I. Search for target antigens in subcellular fractions. Cell. Immunol. 18:304.PubMedCrossRefGoogle Scholar
  10. (10).
    Lemmonier, F., Burakoff, S., Mescher, M., Dorf, M., and Benacerraf, B. 1978. Inhibition of the induction of cytolytic T lymphocytes with alloantisera directed aginst H-2K and H-2D gene products. J. Immunol. 120:1717.Google Scholar
  11. (11).
    Mescher, M., Sherman, L., and Burakoff, S. 1978. The induction of secondary cytolytic T lymphocytes by solubilized membrane proteins. J. Exp. Med. 147:946.PubMedCrossRefGoogle Scholar
  12. (12).
    Fast, L.D, and Fan, D.P. 1978. Dissociated and reconstituted subcellular alloantigen capable of stimulating mouse cytotoxic T lymphocytes in vitro. J. Immunol. 120:1092.PubMedGoogle Scholar
  13. (13).
    Finberg, R., Mescher, M., and Bufakoff, S.J. 1978. The induction of virus-specific cytotoxic T Ijnmphocytes with solubilized viral and membrane proteins. J. Exp. Med. 148:1620.PubMedCrossRefGoogle Scholar
  14. (14).
    Loh, D., Ross, A.H., Hale, A.H., Baltimore, D., and Eisen, H.N. 1979. Synthetic phospholipid vesicles containing a purified viral antigen and cell membrane proteins stimulate the development of cytotoxic T Ijmiphocytes. J. Exp. Med. 150:1067.PubMedCrossRefGoogle Scholar
  15. (15).
    Ciavarra, R.P., Kang, C.Y., and Forman, J. 1980. Vesicular stomatitis antigens recognized by cytotoxic cells: analysis with defective intefering particles and reconstituted membrane vesicles. J. Immimol. 125:336.Google Scholar
  16. (16).
    Alaba, O., and Law, L.W. 1980. Specific induction of syngeneic cytotoxic T lymphocytes by solubilized tumor antigen: fractionation of the specific R-MuLV-induced leukemia antigen. J. Immunol. 125:414.PubMedGoogle Scholar
  17. (17).
    Engelhard, V.H., Strominger, J.L., Mescher, M., and Burakoff, S. 1978. Induction of secondary cytotoxic T lymphocytes by purified HLA-A and HLA-B antigens reconstituted into phospholipid vesicles. Proc. Natl. Acad. Sci. USA 75:5688.PubMedCrossRefGoogle Scholar
  18. (18).
    Herrmann, S.H., and Mescher, M.F. 1979. Purification of the H-2K molecule of the murine major histocompatibility complex. J. Biol. Chem. 254:8713.PubMedGoogle Scholar
  19. (19).
    Stallcup, K.C., Springer, T.A., and Mescher, M.F. 1981. Characterization of an anti-H-2 monoclonal antibody and its use in large scale antigen purification. J. Immunol. 127:923.PubMedGoogle Scholar
  20. (20).
    Herrmann, S.H., and Mescher, M.F. 1981. Secondary cytolytic T lymphocyte stimulation by purified H-2K in liposomes. Proc. Natl. Acad. Sci. USA 78:2488.PubMedCrossRefGoogle Scholar
  21. (21).
    Hale, A.H., Ruebush, M.J., and Harris, D.T. 1980. Elicitation of anti-viral cytotoxic T lymphocytes with purified viral and H-2 antigens. J. Immunol. 125:428.PubMedGoogle Scholar
  22. (22).
    Weinberger, O., Herrmann, S.H., Mescher, M.F., Benacerraf, B., and Burakoff, S.J. 1981. Cellular interactions in the generation of cytotoxic T Ijmiphocyte responses. Analysis of the helper T cell pathway. Eur. J. Immunol. 11:1105.CrossRefGoogle Scholar
  23. (23).
    Herrmann, S.H., Weinberger, O., Burakoff, S.J., and Mescher, M.F. 1981. Analysis of the two-signal requirement for precursor CTL activation using H-2K in liposomes. Manuscript submitted.Google Scholar
  24. (24).
    Lalande, M.E., McCutcheon, M.J., and Miller, R.G. 1980. Quantitative studies on the precursors of cytotoxic lymphocytes. VI. Second signal requirements of specifically activated precursors isolated 12 hours after stimulation. J. Exp. Med. 151:12.PubMedCrossRefGoogle Scholar
  25. (25).
    Teh, H.-S., and Teh, S.-J. 1980. Direct evidence for a two-signal mechanism of cytotoxic T-lymphocyte activation. Nature 285:163.PubMedCrossRefGoogle Scholar
  26. (26).
    Symington, F.W., and Teh, H.-S. 1980. A two-signal mechanism for the induction of cytotoxic T lymphocytes. Scand. J. Immunol. 12:1.PubMedCrossRefGoogle Scholar
  27. (27).
    Bach, F.H., Bach, M.L., and Sondel, P.M. 1976. Differential function of major histocompatibility complex antigens in T lymphocyte activation. Nature 259:273.PubMedCrossRefGoogle Scholar
  28. (28).
    Lafferty, K.L., and Woolnough, J. 1977. The origin and mechanism of the allograft reaction. Immunol. Rev. 35:231.PubMedCrossRefGoogle Scholar
  29. (29).
    Weinberger, O., Herrmann, S.H., Mescher, M.F., Benacerraf, B., and Burakoff, S.J. 1980. Cellular interactions in the generation of cytolytic T lymphocyte responses. Role of la positive splenic adherent cells in the presentation of H-2 antigen. Proc. Natl. Acad. Sci. USA 77. 6091.PubMedCrossRefGoogle Scholar
  30. (30).
    Weinberger, O., Hermann, S.H., Mescher, M.F., Benacerraf, B., and Burakoff, S.J, 1981. Antigen presenting cell function in the induction of helper T cells for cytolytic T Ijrmphocyte responses. Proc. Natl. Acad. Sci. USA 78:1796.PubMedCrossRefGoogle Scholar
  31. (31).
    Herrmann, S.H., and Mescher, M.F. 1981. Lymphocyte recognition of H-2 antigen in liposomes. J. Supramol. Struct, and Cellular Biochem., in press.Google Scholar
  32. (32).
    Mescher, M.F., Jose, M.J.L., and Balk, S.P. 1981. Actin-containing matrix associated with the plasma membrane of murine tumor and Ijnnphoid cells. Nature 289;139.PubMedCrossRefGoogle Scholar
  33. (33).
    Balk, S.P., Walker, J., and Mescher, M.F. 1981. Kinetics of cytolytic T lymphocyte binding to target cells in suspension. J. Immunol. 126:2177.PubMedGoogle Scholar
  34. (34).
    Linna, T.J., Engers, H.D., Cerottini, J.-C., and Brunner, K.T. 1978. Inhibition of cytolytic T Ijrmphocyte activity with subcellular alloantigen preparations and with unlabeled allogeneic target cells. J. Immunol. 120:1544.PubMedGoogle Scholar
  35. (35).
    Martz, E. 1977. Mechanism of specific tumor cell lysis by alioimmune T lymphocytes: resolution and characterization of discrete steps in the cellular interaction. Contemp. Top. Immunobiol. 7:301.PubMedCrossRefGoogle Scholar
  36. (36).
    Balk, S.P., and Mescher, M.F. 1981. Specific reversal of cytolytic T cell-target cell functional binding is induced by free target cells. J. Immunol. 127:51.PubMedGoogle Scholar
  37. (37).
    Balk, S.P., and Mescher, M.F. 1981. Specific reversal of cytolytic T lymphocyte-target cell interaction. J. Supramol. Struct, and Cellular Biochem., in press.Google Scholar
  38. (38).
    Hollander, N., Mehdi, S.Q., Weissman, I.L., McConnnell, H.M., and Kriss, J.P. 1979. Allogeneic cytolysis of reconstituted membrane vesicles. Proc. Natl. Acad. Sci. USA 76:4042.PubMedCrossRefGoogle Scholar
  39. (39).
    Mehdi, S.Q., Lewis, J.T., Copeland, B.R., and McConnell, H.M. 1980. Freeze-fracture of reconstituted model membranes used as targets for cell-mediated cytotoxicity. Biochim. Biophys. Acta. 600:590.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Matthew F. Mescher
    • 1
  • Steven P. Balk
    • 1
  • Steven J. Burakoff
    • 1
  • Steven H. Herrmann
    • 1
  1. 1.Department of Pathology and the Sidney Farber Cancer InstituteHarvard Medical SchoolBostonUSA

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