Abstract
Studies of effector cell biochemical processes associated with cell-mediated cytolysis have been undertaken by numerous laboratories to define the conditions required for the expression of cytolytic activity, and hopefully to ultimately eludicate mechanisms of cytolysis. By such studies, the requirements for the divalent cations, Ca++ and/or Mg++, in T-cell and non-T cell-mediated cytolysis have been demonstrated using chelating agents (1,2). Similar studies of the possible importance of the monovalent cations, Na+ and K+, had been impeded by lack of equally selective means of altering their intracellular concentrations. However, inhibition of cytolytic activity by ouabain (3,4). which specifically blocks membrane active transport of Na+ and K+ (5), indicates that these cations as well may influence cytolytic activity.
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References
Golstein, P., and E.T. Smith. The lethal hit stage of mouse T and non-T cell mediated cytolysis: differences in cation requirements and characterization on an analytical “cation pulse” method. Eur. J. Immunol. 6:31 (1976).
Plaut, M., Bubbers, J.E., and C.S. Henney. Studies on the mechanism of lymphocyte-mediated cytolysis. VII. Two stages in the T cell-mediated lytic cycle with distinct cation requirements. J. Immunol. 116:150 (1976).
Lawrence, E.C., Muchmore, A.V., Dooley, N.J. and R.M. Blaese. Differential effects of ouabain on human cell-mediated cytotoxicity. Cell. Immunol. 46:100 (1979).
Wright, P., and M. De Marchi. Ouabain inhibition of human lymphocyte cytotoxicity. Cell. Immunol. 45:318 (1979).
Skou, J.C. Enzymatic basis for active transport of Na++ and K+ across cell membrane. Physiol. Rev. 45:596 (1965).
Ladisch, S., and S.A. Feig. Submitted, J. Immunol.
Ladisch, S., Feig, S.A., Henderso, T., and B. Berman. Submitted, J. Immunol. Meth.
Kragballe, K., Beck-Nielsen, W., Pedrsen, O., Ellegaard, J., and N.S. Sorensen. Monocyte-mediated antibody-dependent cytotoxicity. Modulation by glycolysis and insulin. Scand. J. Haematol. 26:137 (1981).
Poplack, D.G., Bonnard, G.D., Holiman, B.J., and R.M. Blaese. Monocyte-mediated antibody-dependent cellular cytotoxicity. A clinical test of monocyte function. Blood 48:809 (1976).
Segel, G.B. and M.A. Lichtman. Potassium transport in human blood lymphocytes treated with phytohemagglutinin. J. Clin. Invest. 58:1358 (1976).
Kernan, R.P. Cell K. Butterworth, Washington, pp. 121–141 (1965).
Koller, C.A., Laufman, H.B., and A.F. Lo Buglio. Characterization of monocyte antibody dependent cellular cytotoxicity (ADCC) against RBC targets. Blod 51:152a (1978).
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© 1982 Plenum Press, New York
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Ladisch, S., Ulsh, L., Feig, S.A. (1982). Influence of Monovalent Cation Concentrations on Monocyte-Mediated ADCC. In: Clark, W.R., Golstein, P. (eds) Mechanisms of Cell-Mediated Cytotoxicity. Advances in Experimental Medicine and Biology, vol 146. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8959-0_15
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DOI: https://doi.org/10.1007/978-1-4684-8959-0_15
Publisher Name: Springer, Boston, MA
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