Computer Tracking of Endothelial Activation Responses

  • Una S. Ryan
  • Linda J. Mayfield
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 242)


Endothelial cell responses to stimuli may involve structural, functional or behavioral alterations or a combination of all these.1 Understanding of the role of endothelial cells depends on the ability of investigators to measure these responses. Considerable progress has been made in measuring some, but not all, endothelial responses. Functional responses can usually be quantified by bioassay or by biochemical analysis of endothelial products, enzymes or receptors. Structural responses can be recorded by light and electron microscopy and, to a certain extent, quantification of structural alterations can be achieved by morphometric means. However, analysis of endothelial behavior in terms of migration and division activity has been hampered by existing methods for quantitative assay. Thus, in this paper we have focused on these less well-documented areas. We describe studies that consider methods for measuring endothehal cell behavior.


Endothelial Cell Wound Edge Untreated Culture Denude Area Pulmonary Endothelium 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Una S. Ryan
    • 1
  • Linda J. Mayfield
    • 1
  1. 1.Department of Medicine (R58)University of Miami School of MedicineMiamiUSA

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