Development of Antibodies and Immunological Methods for Terminal Transferase Assay

Bollum, F. J.

doi:10.1007/978-1-4684-8929-3_10

F. J. Bollum³

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 145))

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Abstract

Antibody against homogeneous terminal transferase (1) has turned out to be an essential reagent for biological and biochemical investigations on the occurrence and structure of the enzyme. Carefully prepared antibody preparations have allowed characterization of the normal cells that contain TdT (2–4), analysis of leukemic cell populations (5), and, most recently, new insight into the conservation and structure of terminal transferase peptides (6). Although preparation, characterization and use of monospecific immune reagents is a fairly routine procedure, it is of value to record some of the experience we found to be worthwhile.

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Authors and Affiliations

Biochemistry, USUHS, 4301 Jones Bridge Road, 20814, Bethesda, MD, USA
F. J. Bollum

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F. J. Bollum
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Editors and Affiliations

CNR Institute of Biochemical and Evolutionary Genetics, Pavia, Italy
Umberto Bertazzoni (CEC Official) (CEC Official)
Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
F. J. Bollum

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Bollum, F.J. (1982). Development of Antibodies and Immunological Methods for Terminal Transferase Assay. In: Bertazzoni, U., Bollum, F.J. (eds) Terminal Transferase in Immunobiology and Leukemia. Advances in Experimental Medicine and Biology, vol 145. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8929-3_10

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DOI: https://doi.org/10.1007/978-1-4684-8929-3_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8931-6
Online ISBN: 978-1-4684-8929-3
eBook Packages: Springer Book Archive

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