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The Yeast KEX1 Gene Product Acts as a Carboxypeptidase B-Like Protease in Processing Secreted Protein Precursors

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Book cover Molecular Biology of Brain and Endocrine Peptidergic Systems

Part of the book series: Biochemical Endocrinology ((BIOEND))

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Abstract

A discussion of yeast proteases may seem, at first sight, to be out of place in this book. However, the ease of manipulation of single celled eukaryotes has led to a realization that all eukaryotes have essential similarities, and recent insights from yeast seem very relevant to the problem of protein processing. These developments offer a new direction in solving the longstanding question of the nature of the proteases involved in the maturation of neuropeptides and peptide hormones.

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References

  • Achstetter, T. and Wolf, D.H., 1985, Hormone processing and membrane bound proteases in yeast. EMBQ J. 4:173–177.

    CAS  Google Scholar 

  • Beggs, J.D., 1981, Multiple-copy yeast plasmid vectors. In Molecular Genetics in Yeast, Alfred Benzon Symposium, 16:383–390. Edited by D. von Wettstein, A. Stenderup, M. Kielland-Brandt and J. Friis, Copenhagen: Munksgaard.

    Google Scholar 

  • Bostian, K.A., Elliott, Q., Bussey, H., Burn, V., Smith A. and Tipper, D.J., 1984, Sequence of the preprotoxin dsRNA gene of type I killer yeast: multiple processing events produce a two-component toxin. Cell 36:741–751.

    Article  PubMed  CAS  Google Scholar 

  • Bussey, E., Saville, D., Greene, D., Tipper, D.J. and Bostian, K.A., 1983, Secretion of Saccharomyces cerevisiae killer toxin: processing of the glycosylated precursor. Mol. Cell. Biol. 3:1362–1370.

    PubMed  CAS  Google Scholar 

  • Dmochowska, A., Dignard, D., Henning, D., Thomas, D.Y. and Bussey, H., 1987, Yeast KEX1 gene encodes a putative protease with a carboxypeptidase β-like function involved in killer toxin and α-factor precursor processing. Cell 50:573–584.

    Article  PubMed  CAS  Google Scholar 

  • Fricker, L.D., Evans, C.J., Esch, F.S. and Herbert, E., 1986, Cloning and sequence analysis of cDNA for bovine carboxypeptidase E. Nature 323:461–464.

    Article  PubMed  CAS  Google Scholar 

  • Fuller, R.S., Brake, A. and Thorner, J., 1986, The yeast KEX2, required for processing prepro-α-factor, encodes a calcium-dependent endopeptidase that cleaves after Lys-Arg and Arg-Arg sequences. In Leive, L. (Ed), Microbiology-1986, American Society for Microbiology, pp. 273–278.

    Google Scholar 

  • Fuller, R.S., Sterne, R.E. and Thorner, J., 1988, Enzymes required for yeast prohormone processing. Ann. Rev. Physiol. 50, (in press).

    Google Scholar 

  • Julius, D., Blair, L., Brake, A., Sprague, G. and Thorner, J., 1983, Yeast α-factor is processed from a larger precursor polypeptide: the essential role of a membrane-bound dipeptidyl aminopeptidase. Cell 32:839–852.

    Article  PubMed  CAS  Google Scholar 

  • Julius, D., Brake, A., Blair, L., Kunisawa, R. and Thorner, J., 1984a, Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-α-factor. Cell 37:1075–1089.

    Article  PubMed  CAS  Google Scholar 

  • Julius, D., Schekman, R. and Thorner, J., 1984b, Glycosylation and processing of prepro-α-factor through the yeast secretory pathway. Cell 36:309–318.

    Article  PubMed  CAS  Google Scholar 

  • Kurjan, J. and Herskowitz, I., 1982, Structure of a yeast pheromone gene (MF a): a putative α-factor precursor contains four tandem copies of mature α-factor. Cell 30:933–943.

    Article  PubMed  CAS  Google Scholar 

  • Leibowitz, M. and Wickner, R., 1976, A chromosomal gene required for killer plasmid expression, mating, and spore maturation in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 73:2061–2065.

    Article  PubMed  CAS  Google Scholar 

  • Wagner, J.C. and Wolf, D.H., 1987, Hormone (pheromone) processing enzymes in yeast: the carboxyterminal processing enzyme of the mating pheromone α-factor, carboxypeptidase ysca is absent in α-factor maturation defective kex1 mutant cells. EEBS Letters 221:423–426.

    CAS  Google Scholar 

  • Zhu, H., Bussey, H., Thomas, D.Y., Gagnon, J. and Bell, A., 1987, Determination of the carboxyl termini of the a and β subunits of yeast K1 killer toxin: requirement of a earboxypeptidase β-like activity for maturation. J. Biol. Chem. 262:10728–10732.

    PubMed  CAS  Google Scholar 

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© 1988 Plenum Press, New York

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Cooper, A., Bussey, H. (1988). The Yeast KEX1 Gene Product Acts as a Carboxypeptidase B-Like Protease in Processing Secreted Protein Precursors. In: Chrétien, M., McKerns, K.W. (eds) Molecular Biology of Brain and Endocrine Peptidergic Systems. Biochemical Endocrinology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8801-2_14

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  • DOI: https://doi.org/10.1007/978-1-4684-8801-2_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-8803-6

  • Online ISBN: 978-1-4684-8801-2

  • eBook Packages: Springer Book Archive

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