Abstract
A discussion of yeast proteases may seem, at first sight, to be out of place in this book. However, the ease of manipulation of single celled eukaryotes has led to a realization that all eukaryotes have essential similarities, and recent insights from yeast seem very relevant to the problem of protein processing. These developments offer a new direction in solving the longstanding question of the nature of the proteases involved in the maturation of neuropeptides and peptide hormones.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Achstetter, T. and Wolf, D.H., 1985, Hormone processing and membrane bound proteases in yeast. EMBQ J. 4:173–177.
Beggs, J.D., 1981, Multiple-copy yeast plasmid vectors. In Molecular Genetics in Yeast, Alfred Benzon Symposium, 16:383–390. Edited by D. von Wettstein, A. Stenderup, M. Kielland-Brandt and J. Friis, Copenhagen: Munksgaard.
Bostian, K.A., Elliott, Q., Bussey, H., Burn, V., Smith A. and Tipper, D.J., 1984, Sequence of the preprotoxin dsRNA gene of type I killer yeast: multiple processing events produce a two-component toxin. Cell 36:741–751.
Bussey, E., Saville, D., Greene, D., Tipper, D.J. and Bostian, K.A., 1983, Secretion of Saccharomyces cerevisiae killer toxin: processing of the glycosylated precursor. Mol. Cell. Biol. 3:1362–1370.
Dmochowska, A., Dignard, D., Henning, D., Thomas, D.Y. and Bussey, H., 1987, Yeast KEX1 gene encodes a putative protease with a carboxypeptidase β-like function involved in killer toxin and α-factor precursor processing. Cell 50:573–584.
Fricker, L.D., Evans, C.J., Esch, F.S. and Herbert, E., 1986, Cloning and sequence analysis of cDNA for bovine carboxypeptidase E. Nature 323:461–464.
Fuller, R.S., Brake, A. and Thorner, J., 1986, The yeast KEX2, required for processing prepro-α-factor, encodes a calcium-dependent endopeptidase that cleaves after Lys-Arg and Arg-Arg sequences. In Leive, L. (Ed), Microbiology-1986, American Society for Microbiology, pp. 273–278.
Fuller, R.S., Sterne, R.E. and Thorner, J., 1988, Enzymes required for yeast prohormone processing. Ann. Rev. Physiol. 50, (in press).
Julius, D., Blair, L., Brake, A., Sprague, G. and Thorner, J., 1983, Yeast α-factor is processed from a larger precursor polypeptide: the essential role of a membrane-bound dipeptidyl aminopeptidase. Cell 32:839–852.
Julius, D., Brake, A., Blair, L., Kunisawa, R. and Thorner, J., 1984a, Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-α-factor. Cell 37:1075–1089.
Julius, D., Schekman, R. and Thorner, J., 1984b, Glycosylation and processing of prepro-α-factor through the yeast secretory pathway. Cell 36:309–318.
Kurjan, J. and Herskowitz, I., 1982, Structure of a yeast pheromone gene (MF a): a putative α-factor precursor contains four tandem copies of mature α-factor. Cell 30:933–943.
Leibowitz, M. and Wickner, R., 1976, A chromosomal gene required for killer plasmid expression, mating, and spore maturation in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 73:2061–2065.
Wagner, J.C. and Wolf, D.H., 1987, Hormone (pheromone) processing enzymes in yeast: the carboxyterminal processing enzyme of the mating pheromone α-factor, carboxypeptidase ysca is absent in α-factor maturation defective kex1 mutant cells. EEBS Letters 221:423–426.
Zhu, H., Bussey, H., Thomas, D.Y., Gagnon, J. and Bell, A., 1987, Determination of the carboxyl termini of the a and β subunits of yeast K1 killer toxin: requirement of a earboxypeptidase β-like activity for maturation. J. Biol. Chem. 262:10728–10732.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Plenum Press, New York
About this chapter
Cite this chapter
Cooper, A., Bussey, H. (1988). The Yeast KEX1 Gene Product Acts as a Carboxypeptidase B-Like Protease in Processing Secreted Protein Precursors. In: Chrétien, M., McKerns, K.W. (eds) Molecular Biology of Brain and Endocrine Peptidergic Systems. Biochemical Endocrinology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8801-2_14
Download citation
DOI: https://doi.org/10.1007/978-1-4684-8801-2_14
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8803-6
Online ISBN: 978-1-4684-8801-2
eBook Packages: Springer Book Archive