Abstract
The mechanisms by which gene expression is controlled in time, in a cell type specific manner and in response to physiologically active agents is currently under intense study using a variety of strategies. We have utilized an approach to this problem which offers several attractive features. This involves the identification of specific variation in a pattern of gene expression within a group of related species followed by identification of the DNA sequence elements which operate to control expression of the gene using functional tests. Subsequently, a comparison of the nucleotide sequence of these regions from each member of the species set should lead to hypotheses about specific nucleotides having a role in the control of at least one aspect of the gene’s pattern of expression. This hypothesis depends on the assumptions that sequence elements which are demonstrably conserved in different species are similar due to selection for a function, and that sequence differences may be regarded as candidates for the basis of the expression differences. Each of these can be tested by appropriate in vitro mutagenesis studies.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Atkinson, P. W., Mills, L. E., Starmer W. T., and Sullivan, D. T., 1988, Structure and evolution of the Adh genes of Drosophila mojavensis, Genetics 120:713–723.
Batterham, P., Starmer, W. T., and Sullivan, D. T., 1982, Biochemical genetics of the alcohol longevity response of Drosophila mojavensis, in: Ecological Genetics and Evolution: The Cactus-yeast-Drosophila Model System (J. S. F. Barker, and W. T. Starmer, eds), Academic Press Australia, Sydney, pp. 307–321.
Batterham, P., Chambers, G. K., Starmer, W. T., and Sullivan, D. T., 1984, Origin and expression of an alcohol dehydrogenase gene duplication in the genus Drosophila, Evolution 38:644–657.
Batterham, P., Gritz, E., Starmer, W. T., and Sullivan, D. T., 1983a, Biochemical characterization of the products of the Adh loci of Drosophila mojavensis, Biochem. Genet. 21:871–883.
Batterham, P., Lovett, J., Starmer, W. T., and Sullivan D., 1983b, Differential regulation of duplicate alcohol dehydrogenase genes in D. mojavensis, Devl Biol. 96:346–354.
Benyajati, C., Spoerel, H., Haymerle, H., and Ashburner, M., 1983, The messenger RNA for Adh in D. melanogaster differs in its 5’ end in different developmental stages, Cell 33:125–133.
Fischer, J., and Maniatis, T., 1985, Structure and transcription of the Drosophila mulleri alcohol dehydrogenase genes, Nucl. Acids Res. 13:6899–6917.
Mills, L. E., Batterham, P., Alegre, J., Starmer, W. T., and Sullivan, D. T., 1986, Molecular genetic characterization of a locus that contains duplicate Adh genes in Drosophila mojavensis and related species, Genetics 112:295–310.
Mismer, D., and Rubin, G. M., 1987, Analysis of the promoter of the ninaE opsin gene in Drosophila melanogaster, Genetics 116:565–578.
Oakeshott, J. G., Chambers, G. K., East, P. D., Gibson, J. B., and Barker, J.S.F., 1982, Evidence for a genetic duplication involving alcohol dehydrogenase genes in Drosophila huzzatii and related species, Aust. J. biol. Sci. 35:73–84.
Savakis, C., Ashburner, M., and Willis, J. H., 1986, The expression of the gene coding for alcohol dehydrogenase during the development of Drosophila melanogaster, Devl Biol. 114:194–207.
Steller, M., and Pirrotta, V., 1985, A transposable P vector that confers selectable G418 resistance to Drosophila larva, EMBO J. 4:107–171.
Wasserman, M.,1982, Evolution of the repleta group, in: Genetics and Biology of Drosophila, Vol. 3B (M. Ashburner, H. L. Carson, and J. N. Thompson, eds), Academic Press, New York, pp. 61–139.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer Science+Business Media New York
About this chapter
Cite this chapter
Sullivan, D.T., Atkinson, P.W., Bayer, C.A., Menotti-Raymond, M.A. (1990). The Evolution of Adh Expression in the Repleta Group of Drosophila. In: Barker, J.S.F., Starmer, W.T., MacIntyre, R.J. (eds) Ecological and Evolutionary Genetics of Drosophila . Monographs in Evolutionary Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8768-8_26
Download citation
DOI: https://doi.org/10.1007/978-1-4684-8768-8_26
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8770-1
Online ISBN: 978-1-4684-8768-8
eBook Packages: Springer Book Archive