Abstract
Cells obtained by enzymatic dissociation of stage 28–30 (1) chick embryo tibiae cultured adherent to plastic culture dishes, dedifferentiate and assume a fibroblast-like morphology. Dedifferentiated cells synthesize extracellular matrix proteins characteristic of committed prechondrogenic mesenchymal cells (i.e. type I collagen and fibronectin). Transfer of dedifferentiated cells to suspension culture induce their differentiation to single hypertrophic chondrocytes after a transient stage in which cells form aggregates.
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References
V. Hamburger and H. L. Hamilton, A series of normal stages in the development of the chick embryo, J. Morphol. 88:49 (1951).
P. Castagnola, G. Moro, F. Descalzi-Cancedda and R. Cancedda, Type X collagen synthesis during in vitro development of chick embryo tibial chondrocytes, J. Cell. Biol. 102:2310 (1986).
C. Tacchetti, R. Quarto, L. Nitsch, D. J. Hartmann and R. Cancedda, In vitro morphogenesis of chick embryo endochondral cartilage, submitted for publication (1987).
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© 1988 Plenum Press, New York
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Tacchetti, C., Quarto, R., Cancedda, R. (1988). Collagen Distribution in Endochondral Cartilage Obtained in Vitro from Dedifferentiated Cells. In: Nicoletti, B., Kopits, S.E., Ascani, E., McKusick, V.A., Dryburgh, S.C. (eds) Human Achondroplasia. Basic Life Sciences, vol 48. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8712-1_13
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DOI: https://doi.org/10.1007/978-1-4684-8712-1_13
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