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Acetylcholine Metabolism in PC12, A Clonal Cell Line on Secretory Cells

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Cholinergic Mechanisms

Part of the book series: Advances in Behavioral Biology ((ABBI,volume 25))

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Abstract

PC12 is a clonal line of rat pheochromocytoma (7). PC12 cells synthesize catecholamines (primarily dopamine) and acetylcholine (ACh), store each in different granules and exhibit a Ca++-sensitive, depolarization-induced secretion of each (8,9,15,19). These properties make it possible to compare the metabolism and granular storage of two different transmitters produced by the same secretory cell. In this paper we summarize our use of PC12 cells for such studies, with the emphasis being on ACh.

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Author information

Authors and Affiliations

  1. Departments of Biological Chemistry, School of Medicine, University of California, Los Angeles, California, 90024, USA

    B. D. Howard

  2. Departments of Pharmacology, School of Medicine, University of California, Los Angeles, California, 90024, USA

    W. P. Melega

Authors
  1. B. D. Howard
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  2. W. P. Melega
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Editor information

Editors and Affiliations

  1. Institute of Pharmacology, University of Florence, Florence, Italy

    Giancarlo Pepeu

  2. Mario Negri Institute for Pharmacological Research, Milan, Italy

    Herbert Ladinsky

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© 1981 Plenum Press, New York

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Howard, B.D., Melega, W.P. (1981). Acetylcholine Metabolism in PC12, A Clonal Cell Line on Secretory Cells. In: Pepeu, G., Ladinsky, H. (eds) Cholinergic Mechanisms. Advances in Behavioral Biology, vol 25. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8643-8_12

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  • DOI: https://doi.org/10.1007/978-1-4684-8643-8_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-8645-2

  • Online ISBN: 978-1-4684-8643-8

  • eBook Packages: Springer Book Archive

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