Some Techniques of Radioactive Gas Chromatography for Lipid Research
By definition, lipids are “high boilers,”—and thereby hangs a tale. Historically, the story begins with the fact that when gas chromatographic (GLC) techniques were developed for petroleum products they could not be transferred directly to lipid problems. For example, materials used for the construction of insulators which were suitable for hydrocarbons at 100°C, melted at 250°C; problems of condensation in gas-stream conduction tubes, which are inconsequential in the low-temperature ranges, become critical for high-boiling compounds; and adsorption effects unnoticed at low temperatures are of limited applicability for low-vapor-pressure materials. Necessary modifications in GLC techniques have now been developed to meet the problem encountered with high-boiling, radioactive lipid compounds. Particular emphasis will be given to tandem monitoring of GLC for radioactivity.
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