Abstract
Gas-liquid chromatography (GLC), developed by James and Martin [1], is now recognized as the most powerful technique available for the separation of volatile substances of a homologous series. It is used not only for the analysis of the composition of natural products, but is also employed with increasing frequency for the identification of substances. It is a particularly valuable tool for biochemists studying biosynthetic processes, as its speed and sensitivity enable one to identify with considerable confidence new products on a minute scale. As biochemists often use isotopically labeled compounds in their investigations, the problem of simultaneous gas-liquid chromatographic analysis and monitoring of the chromatographic vapors for a radioactive isotope arose quite naturally. It is a common practice in many laboratories to use some kind of automatic device for the scanning of paper chromatograms for radioactive spots, but the monitoring of the effluent from gas-liquid chromatographic columns for a radioactive isotope has been achieved so far only in a few laboratories.
Presented at the Fifth Symposium on Advances in Tracer Methodology, 1961.
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References
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© 1963 New England Nuclear Corporation
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Popjak, G., Lowe, A.E., Moore, D. (1963). Simultaneous Measurement of C14 and H3 during Gas-Liquid Chromatography. In: Rothchild, S. (eds) Advances in Tracer Methodology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8619-3_24
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DOI: https://doi.org/10.1007/978-1-4684-8619-3_24
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