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Are the Multiple Signal Transduction Pathways of the Pho Regulon Due to Cross Talk or Cross Regulation?

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Regulation of Gene Expression in Escherichia coli

Abstract

The phosphate (Pho) regulon includes a large number of coregulated genes whose expression is controlled by the environmental (extracellular) inorganic phosphate (Pi) level. Altogether 11 Pho regulon promoters for a total of 38 different genes have now been characterized in E. coli or closely related bacteria (Fig. 15.1). The expression of most of them has been shown to require the transcription factor PhoB that upon phosphorylation activates transcription by binding to Pho Box sequences within the respective promoter region of each of these genes or operons. The E. coli Pho regulon currently comprises 31 genes that are arranged in eight unlinked transcriptional units. At least 13 of these genes are also believed to exist in Salmonella typhimurium, although 16 others are known to be absent. In addition, S. typhimurium has seven Pho regulon genes that are absent in E. coli. Most of the corresponding gene products have roles in the use of various phosphorus (P) compounds as sole P sources for growth, while the roles of others are unknown.1

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References

  1. Wanner BL. Phosphorus assimilation and control of the phosphate regulon. In: Neidhardt FC, Curtiss RI, Gross CA et al, eds. Escherichia coli and Salmonella typhimurium cellular and molecular biology. 2nd ed, chapter 84. Washington, DC: Am Soc Microbiol, 1996; (in press).

    Google Scholar 

  2. Jiang W, Metcalf WW, Leeks KS et al. Molecular cloning, mapping and regulation of Pho regulon genes for phosphonate breakdown by the phosphonatase pathway of Salmonella typhimurium LT2. J Bacteriol 1995; 177:6411–26.

    Google Scholar 

  3. Horiuchi T, Horiuchi S, Mizuno D. A possible negative feedback phenomenon controlling formation of alkaline Phosphomonoesterase in Escherichia coli. Nature 1959; 183:1529–30.

    Article  Google Scholar 

  4. Torriani A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta 1960; 38:460–9.

    Article  Google Scholar 

  5. Wanner BL. Signal transduction and cross regulation in the Escherichia coli phosphate regulon involving the phosphate sensor PhoR, the catabolite regulatory sensor CreC, and acetyl phosphate. In: Hoch JA, Silhavy TJ, eds. Two-component signal tranduction. Washington, DC: Am Soc Microbiol, 1995:203–21.

    Google Scholar 

  6. Ronson CW, Nixon BT, Ausubel FM. Conserved domains in bacterial regulatory proteins that respond to environmental stimuli. Cell 1987; 49:579–81.

    Article  Google Scholar 

  7. Parkinson JS, Kofoid EC. Communication modules in bacterial signaling proteins. Annu Rev Genet 1992; 26:71–112.

    Article  Google Scholar 

  8. Swanson RV, Alex LA, Simon MI. Histidine and aspartate phosphorylation: Two-component systems and the limits of homology. Trends Biochem Sci 1994; 19:485–90.

    Article  Google Scholar 

  9. Herskowitz I. MAP kinase pathways in yeast: for mating and more. Cell 1995; 80:187–97.

    Article  Google Scholar 

  10. Wanner BL, Wilmes-Riesenberg MR. Involvement of phosphotrans-acetylase, acetate kinase, and acetyl phosphate synthesis in the control of the phosphate regulon in Escherichia coli. J Bacteriol 1992; 174:2124–30.

    Google Scholar 

  11. Wanner BL. Minireview. Is cross regulation by phosphorylation of two-component response regulator proteins important in bacteria? J Bacteriol 1992; 174:2053–8.

    Google Scholar 

  12. Wanner BL. Gene regulation by phosphate in enteric bacteria. J Cell Biochem 1993; 51:47–54.

    Article  Google Scholar 

  13. Wanner BL. Multiple controls of the Escherichia coli Pho regulon by the Pi sensor PhoR, the catabolite regulatory sensor CreC, and acetyl phosphate. In: Torriani-Gorini A, Yagil E, Silver S, eds. Phosphate in Microorganisms, Cellular and Molecular Biology. Washington, DC: Am Soc Microbiol, 1994:13–21.

    Google Scholar 

  14. Garen A, Echols H. Genetic control of induction of alkaline phosphatase synthesis in E. coli. Proc Natl Acad Sci USA 1962; 48:1398–402.

    Article  Google Scholar 

  15. Garen A, Echols H. Properties of two regulating genes for alkaline phosphatase. J Bacteriol 1962; 83:297–300.

    Google Scholar 

  16. Wanner BL. Phosphate regulation of gene expression in Escherichia coli. In: Neidhardt FC, Ingraham J, Low KB et al. eds. Escherichia coli and Salmonella typhimurium cellular and molecular biology, Volume 2. Washington, DC: Am Soc Microbiol, 1987:1326–33.

    Google Scholar 

  17. Steed PM, Wanner BL. Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU Operon: evidence of a new role for the PhoU protein in the phosphate regulon. J Bacteriol 1993; 175:6797–809.

    Google Scholar 

  18. Willsky GR, Bennett RL, Malamy MH. Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation. J Bacteriol 1973; 113:529–39.

    Google Scholar 

  19. Shulman RG, Brown TR, Ugurbil K et al. Cellular applications of 31P and 13C nuclear magnetic resonance. Science 1979; 205:160–6.

    Article  Google Scholar 

  20. Cox GB, Webb D, Godovac-Zimmermann J et al. Arg-220 of the PstA protein is required for phosphate transport through the phosphate-specific transport system in Escherichia coli but not for alkaline phosphatase repression. J Bacteriol 1988; 170:2283–6.

    Google Scholar 

  21. Willsky GR, Malamy MH. Characterization of two genetically separable inorganic phosphate transport system in Escherichia coli. J Bacteriol 1980; 144:356–65.

    Google Scholar 

  22. Wanner BL, Latterell P. Mutants affected in alkaline phosphatase expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli. Genetics 1980; 96:242–66.

    Google Scholar 

  23. Amemura M, Makino K, Shinagawa H et al. Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2. J Bacteriol 1990; 172:6300–7.

    Google Scholar 

  24. Wanner BL. Control of phoR-dependent bacterial alkaline phosphatase clonal variation by the phoM region. J Bacteriol 1987; 169:900–3.

    Google Scholar 

  25. White SH, Wimley WC. Peptides in bilayers: structural and thermodynamic basis for partitioning and folding. Curr Opin Struct Biol 1994; 4:79–86.

    Article  Google Scholar 

  26. Makino K, Shinagawa H, Amemura M et al. Signal transduction in the phosphate regulon of Escherichia coli involves phosphotransfer between PhoR and PhoB proteins. J Mol Biol 1989; 210:551–9.

    Article  Google Scholar 

  27. Shinagawa H, Makino K, Yamada M et al. Signal transduction in the phosphate regulon of Escherichia coli: dual functions of PhoR as a protein kinase and a protein phosphatase. In: Torriani-Gorini A, Yagil E, Silver S, eds. Phosphate in Microorganisms. Washington, DC: Am. Soc Microbiol, 1994:285–9.

    Google Scholar 

  28. Makino K, Amemura M, Kim S-K et al. Mechanism of transcriptional activation of the phosphate regulon in Escherichia coli. In: Torriani-Gorini A, Yagil E, Silver S, eds. Phosphate in Microorganisms, Cellular and Molecular Biology. Washington, DC: Am Soc Microbiol, 1994:5–12.

    Google Scholar 

  29. Makino K, Shinagawa H, Amemura M et al. Regulation of the phosphate regulon of Escherichia coli: activation of pstS transcription by PhoB protein in vitro. J Mol Biol 1988; 203:85–95.

    Article  Google Scholar 

  30. Inouye H, Pratt C, Beckwith J et al. Alkaline phosphatase synthesis in a cell-free system using DNA and RNA templates. J Mol Biol 1977; 110:75–87.

    Article  Google Scholar 

  31. Pratt C. Kinetics and regulation of cell-free alkaline phosphatase synthesis. J Bacteriol 1980; 143:1265–74.

    Google Scholar 

  32. Wanner BL, Sarthy A, Beckwith JR. Escherichia coli pleiotropic mutant that reduces amounts of several periplasmic and outer membrane proteins. J Bacteriol 1979; 140:229–39.

    Google Scholar 

  33. Adhya S. Obituary. Harrison Echols (1933–1993). Cell 1993; 73:833–4.

    Article  Google Scholar 

  34. Englesberg E, Irr J, Power J et al. Positive control of enzyme synthesis by gene C in the L-arabinose system. J Bacteriol 1965; 90:946–57.

    Google Scholar 

  35. Englesberg E, Sheppard D, Squires C et al. An analysis of “revertants” of a deletion mutant in the C gene of the L-arabinose gene complex in Escherichia coli B/r: isolation of initiator constitutive mutants (Ic). J Mol Biol 1969; 43:281–98.

    Article  Google Scholar 

  36. Schleif R. 91. The L-Arabinose Operon. In: Neidhardt FC, Ingraham JL, Low KB et al. eds. Escherichia coli and Salmonella typhimurium. Cellular and Molecular Biology, Volume 2. Washington, D.C.: Am Soc Microbiol, 1987:1473–81.

    Google Scholar 

  37. Bracha M, Yagil E. A new type of alkaline phosphatase-negative mutants in Escherichia coli K12. Mol Gen Genet 1973; 122:53–60.

    Article  Google Scholar 

  38. Morris H, Schlesinger MJ, Bracha M et al. Pleiotropic effects of mutations involved in the regulation of Escherichia coli K-12 alkaline phosphatase. J Bacteriol 1974; 119:583–92.

    Google Scholar 

  39. Brickman E, Beckwith J. Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and Φ80 transducing phages. J Mol Biol 1975; 96:307–16.

    Article  Google Scholar 

  40. Willsky GR, Malamy MH. Control of the synthesis of alkaline phosphatase and the phosphate-binding protein in Escherichia coli. J Bacteriol 1976; 127:595–609.

    Google Scholar 

  41. Wanner BL, Bernstein J. Determining the phoM map location in Escherichia coli K-12 by using a nearby transposon Tn10 Insertion. J Bacteriol 1982; 150:429–32.

    Google Scholar 

  42. Hunt AG, Hong J-S. A micromethod for the measurement of acetyl phosphate and acetyl coenzyme A. Anal Biochem 1980; 108:290–4.

    Article  Google Scholar 

  43. Nixon BT, Ronson CW, Ausubel FM. Two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and ntrC. Proc Natl Acad Sci USA 1986; 83:7850–4.

    Article  Google Scholar 

  44. Skarstad K, Thny B, Hwang DS et al. A novel binding protein of the origin of the Escherichia coli chromosome. J Biol Chem 1993; 268:5365–70.

    Google Scholar 

  45. Wanner BL, Wilmes MR, Young DC. Control of bacterial alkaline phosphatase synthesis and variation in an Escherichia coli K-12 phoR mutant by adenyl cyclase, the cyclic AMP receptor protein, and the phoM Operon. J Bacteriol 1988; 170:1092–102.

    Google Scholar 

  46. Wanner BL, Wilmes MR, Hunter E. Molecular cloning of the wild-type phoM operon in Escherichia coli K-12. J Bacteriol 1988; 170:279–88.

    Google Scholar 

  47. Wanner BL. Phosphorus assimilation and its control of gene expression in Escherichia coli. In: Hauska G, Thauer R, eds. The molecular basis of bacterial metabolism. Heidelberg: Springer-Verlag, 1990:152–63.

    Google Scholar 

  48. Iuchi S, Lin ECC. arcA (dye), a global regulatory gene in Escherichia coli mediating repression of enzymes in aerobic pathways. Proc Natl Acad Sci USA 1988; 85:1888–92.

    Article  Google Scholar 

  49. Kakuda H, Hosono K, Shiroishi K et al. Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli. J Biochem (Tokyo) 1994; 116:916–22.

    Google Scholar 

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Authors
  1. Barry L. Wanner
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  2. Weihong Jiang
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  3. Soo-Ki Kim
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  4. Sayaka Yamagata
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  5. Andreas Haldimann
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  6. Larry L. Daniels
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© 1996 R.G. Landes Company

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Wanner, B.L., Jiang, W., Kim, SK., Yamagata, S., Haldimann, A., Daniels, L.L. (1996). Are the Multiple Signal Transduction Pathways of the Pho Regulon Due to Cross Talk or Cross Regulation?. In: Regulation of Gene Expression in Escherichia coli . Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8601-8_15

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  • DOI: https://doi.org/10.1007/978-1-4684-8601-8_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-8603-2

  • Online ISBN: 978-1-4684-8601-8

  • eBook Packages: Springer Book Archive

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