Abstract
Adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4.) catalyzes the irreversible hydrolytic deamination of adenosine to produce inosine and ammonia. Markedly reduced or absent adenosine deaminase activity in man is associated with an autosomal recessive form of severe combined immunodeficiency disease and the relationship appears to be causal.1,2,3,4 Affected patients present a defect of both cellular and humoral immunity characterized clinically by severe recurrent infections with a uniformly fatal outcome if untreated.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
E. R. Giblett, J. E. Anderson, F. Cohen, B. Pollara and H. J. Meuwissen, Adenosine deaminase deficiency in two patients with severely impaired cellular immunity Lancet 2:1067–1069 (1972).
J. Dissing and B. Knudsen, Adenosine deaminase deficiency and combined immunodeficiency syndrome Lancet 2: 1316 (1972).
R. Parkman, E. W. Gelfand, F. S. Rosen, A. Sanderson and R. Hirschhorn, Severe combined immunodeficiency and adenosine deaminase deficiency New Eng. J. Med. 292:714–719 (1975).
H. J. Meuwissen, B. Pollara and R. J. Pickering, Combined immunodeficiency disease associated with adenosine deaminase deficiency J. Pediatrics 86:169–181 (1975).
S. H. Chen, C. R. Scott and K. R. Swedberg, Adenosine deaminase deficiency: expression of the enzyme in cultured skin fibroblasts and amniotic fluid cells, Am. J. Hum. Genet. 27:46–53 (1975).
R. Hirschhorn, N. Beratis and F. S. Rosen, Characterization of residual enzyme activity in fibroblasts from patients with adenosine deaminase deficiency and combined immunodeficiency: evidence for a mutant enzyme Proc. Natl. Acad. Sci. 73:213–217 (1976).
R. Hirschhorn, F. Martiniuk and F. S. Rosen, Adenosine deaminase activity in normal tissues and tissues from a child with severe combined immunodeficiency and adenosine deaminase deficiency Clin. Immunol. and Immunopath. 9:287–292 (1978).
D. A. Carson, R. Goldblum and J. E. Seegmiller, Quantitative immunoassay of adenosine deaminase in combined immunodeficiency disease J. Immunol. 118:270–273 (1977).
P. E. Daddona and W. N. Kelley, Human adenosine deaminase: purification and subunit structure J. Biol. Chem. 252:110–115 (1977).
W. P. Schrader, A. R. Stacy and B. Pollara, Purification of human erythrocyte adenosine deaminase by affinity column chromatography J. Biol. Chem. 251:4026–4032 (1976).
D. Rodbard, W. Bridson and P. L. Payford, Rapid calculation of radioimmunoassay results J. Lab. and Clin. Med. 74:770–781 (1969).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1980 Plenum Press, New York
About this chapter
Cite this chapter
Daddona, P.E., Frohman, M.A., Kelley, W.N. (1980). Radioimmunochemical Analysis of Human Erythrocyte Adenosine Deaminase. In: Rapado, A., Watts, R.W.E., De Bruyn, C.H.M.M. (eds) Purine Metabolism in Man—III. Advances in Experimental Medicine and Biology, vol 122B. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8559-2_27
Download citation
DOI: https://doi.org/10.1007/978-1-4684-8559-2_27
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8561-5
Online ISBN: 978-1-4684-8559-2
eBook Packages: Springer Book Archive