Abstract
A considerable amount of evidence exists to suggest that a rise in cytoplasmic free calcium concentration, [Ca2+]i, evokes morphological, metabolic and secretory responses in endothelial cells in response to stimulation by many inflammatory mediators. One of the most potent agents for stimulating the release of the vasodilators endothelial-derived relaxing factor (EDRF) and prostacyclin (PGI2) is the calcium ionophore A23187. However, the hypothesis that elevated [Ca2+]i is the physiological messenger is held with some caution since A23187-evoked rises in [Ca2+]i sufficient to evoke these responses can conceivably be far larger than those physiologically attained on stimulation with inflammatory mediators. With the development of the fluorescent [Ca2+] indicator dyes quin2, fura-2 and indo-1, increases in [Ca2+]. have been observed in endothelial cells from bovine or porcine aortae or from human umbilical vein in response to histamine, bradykinin, thrombin, adenine nucleotides and PAF. Furthermore, recent work has shown that these mediator-evoked rises in [Ca2+]i are both sufficient and necessary for the production and release of PGI2 (Hallam et al., 1988c). Our attention has now focussed on the mechanisms of Ca2+ movement and the sources of trigger Ca2+.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Adams, D.J., Lategan, T.W., Lodge, N.J. and van Breemen, C. Inward rectifying K+ channels and thrombin-activated cation channels in cultured endothelial cells from bovine pulmonary artery. J. Physiol. 394:45, 1987.
Benham, C.D. and Tsien, R.W. A novel receptor-operated Ca2+-permeable channel activated by ATP in smooth muscle. Nature. 328:275–278, 1987.
Bussolino, F., Aglieita, M., Sanavaio, F., Stacchini, A., Lauri, D. and Camussi, G. Alkyl-ether phosphoglycerides influence calcium fluxes into human endothelial cells. J. Immunol. 135:2748–2753, 1985.
Crutchley, D.J., Ryan, J.W., Ryan, U.S. and Fisher, G.H. Bradykinin-induced release of prostacyclin and thromboxanes from bovine pulmonary artery endothelial cells. Studies with lower homologs and calcium antagonists. Biochim. Biophys. Acta. 751:99–107, 1983.
D’Amore, P. and Shepro, D. Stimulation of growth and calcium influx in cultured bovine, aortic endothelial cells by platelets and vasoactive substances. J. Cell Physiol. 92: 177–184, 1977.
Guth, P.H. and Hirabayashi, K. The effect of histamine on microvascular permeability in the muscularis externa of rat small intestine. Microvasc. Res. 25:322–332, 1983.
Hallam, T.J. and Pearson, J.D. Exogenous ATP raises cytoplasmic free calcium in fura-2 loaded piglet aortic endothelial cells. FEBS Letts. 207:95–99, 1986.
Hallam, T.J. and Rink, T.J. Agonists stimulate divalent cation channels in the plasma membrane of human platelets. FEBS Letts. 186:175–179, 1985.
Hallam, T.J., Jacob, R. and Merritt, J.E. Evidence that agonists stimulate divalent cation influx into human endothelial cells. Biochem. J. 255:179–184, 1988a.
Hallam, T.J., Jacob, R. and Merritt, J.E. Influx of divalent cations into the cytoplasm of human endothelial cells can be independent of receptor stimulation. Biochem. J. 259:125–129, 1989.
Hamilton, K.K. and Sims, P.J. Changes in cytosolic Ca2+ associated with von Willebrand factor release in human endothelial cells exposed to histamine. Study of microcarrier cell monolayers using the fluorescent probe indo-1. J. Clin. Invest. 79:600–608, 1987.
Irvine R.F. and Moore, R.M. Micro-injection of inositol 1,3,4,5-tetrakisphosphate activates sea urchin eggs by a mechanism dependent on external Ca2+. Biochem. J. 240:917–920, 1986.
Jacob, R., Merritt, J.E., Hallam, T.J. and Rink, T.J. Repetitive spikes in cytoplasmic calcium evoked by histamine in human endothelial cells. Nature. 335:40–45, 1988.
Johns, A., Lategan, T.W., Lodge, N.J.., Ryan, U.S., van Breemen, C. and Adams, D.J. Calcium entry through receptor-operated channels in bovine pulmonary artery endothelial cells. Tissue and Cell. 733–745, 1987.
Killackey, J.J.F., Johnston, M.G. and Movat, H.Z. Increased permeability of microcarrier-cultured endothelial monolayers in response to histamine and thrombin. A model for the in vitro study of increased vasopermeability. Am. J. Pathol. 122:50–61, 1986.
Lambert, T.L., Kent, R.S. and Whorton, A.R. Bradykinin stimulation of inositol polyphosphate production in porcine aortic endothelial cells. J. Biol. Chem. 261:15288–15293, 1986.
Lansman, J.B., Hallam, T.J. and Rink, T.J. Single stretch-activated ion channels in vascular endothelial cells as mechanotransducers? Nature. 325:811–813, 1987.
McIntyre, T.M„ Zimmerman, G.A., Satoh, K. and Prescott, S.M. Cultured endothelial cells synthesise both PAF and prostacyclin in response to histamine, bradykinin and ATP. J. Clin. Invest. 76:271–280, 1985.
Resink, T.J., Gregorian, G.Y., Moldabaeva, A.K., Danilov, S.M. and Buhler, F. Histamine-induced phosphoinositide metabolism in cultured human umbilical vein endothelial cells. Association with thromboxane and prostacyclin release. Biochem. Biophys. Res. Comm. 144:438–446, 1987.
Rotrosen, D. and Gallin, J.I. Histamine Type I receptor occupancy increases endothelial cell cytosolic calcium, reduces F-actin, and promotes albumin diffusion across cultured endothelial monolayers. J. Cell Biol. 103:2379–2387, 1986.
Rubanyi, G.M., Schwartz, A.M. and Vanhoutte, P.M. The calcium channel agonists Bay K8644 and (+)202,791 stimulate the release of EDRF from canine femoral arteries. Eur. J. Pharmacol. 117:143–144, 1985.
Spedding, M., Schini, V., Schoeffter, P. and Miller, R.C. Calcium channel activation does not increase release of EDRF in rat aorta although tonic release of EDRF may modulate calcium channel activity in smooth muscle. J. Cardiovasc. Pharmol. 8:1130–1137, 1986.
von Tscharner, V., Prod’hom, B., Baggiolini, M. and Reuter, H. Ion channels in human neutrophils activated by a rise in free cytosolic calcium concentration. Nature 324:369–372, 1986.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Plenum Press, New York
About this chapter
Cite this chapter
Hallam, T.J., Merritt, J.E., Rink, T.J., Jacob, R. (1989). Agonist Stimulated Changes in Human Endothelial Cell Cytosolic Calcium. In: Catravas, J.D., Gillis, C.N., Ryan, U.S. (eds) Vascular Endothelium. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8532-5_16
Download citation
DOI: https://doi.org/10.1007/978-1-4684-8532-5_16
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8534-9
Online ISBN: 978-1-4684-8532-5
eBook Packages: Springer Book Archive